Paired <i>mycobacteria</i>-specific PB and MBC responses in healthy community controls.

<p>Matched frequencies of antigen-specific PBs and MBCs were determined by 6-hour ex-vivo and 6-day in-vitro ELISPOTs respectively. Matched frequencies of PPD- (<b>A</b>), ESAT-6- (<b>B</b>), CFP-10- (<b>C</b>), Ag85A- (<b>D</b>) and Ag85B- (<b>E</b>) specific PBs and MBCs in healthy community controls are shown. Open square across all panels represents an interesting donor who presented with a higher PB:MBC ratio on various antigens tested. Frequencies of antigen-specific PBs and MBCs are presented as antibody secreting cells per million PBMCs.</p

Correlations among paired antigen-specific PB and MBC responses detected in the community controls and TB patients.

<p>Correlations among paired antigen-specific PB and MBC responses detected in the community controls and TB patients.</p

<i>Mycobacteria</i>-specific PB and MBC responses in the active TB patients and community controls.

<p>Frequencies of antigen-specific PBs and MBCs were determined by 6-hour ex-vivo and 6-day in-vitro ELISPOTs respectively. Frequencies of antigen-specific PBs and MBCs are presented as antibody secreting cells per million PBMCs. (<b>A</b>) Frequencies of mycobacteria-specific PBs in the active TB patients (filled circles), community controls responding to ESAT-6/CFP-10 (half filled triangles) and healthy community controls lacking ESAT-6 and CFP-10 responses (open circles) (<b>B</b>) Frequencies of mycobacteria-specific MBCs in the active TB patients (filled circles), community controls responding to ESAT-6/CFP-10 (half filled triangles) those without ESAT-6 and CFP-10 responses (open circles). **** P<0.0001, ***, P<0.001; **, P<0.01.</p

Baseline characteristics at enrolment (during pregnancy) for the mothers of the children examined at age 5 years.

<p>Baseline characteristics at enrolment (during pregnancy) for the mothers of the children examined at age 5 years.</p

Anti-CFP (culture filtrate protein)-10 & anti-ESAT (early secretory antigenic target)-6 antibody concentrations in QFT-GIT mitogen control supernatants strongly correlate with those in QFT-GIT nil control supernatants and there are no statistical differences between them.

<p>Panel A: scatter plots showing correlation. The coefficient (r) and the p values shown correspond to results from Spearman’s rank correlation. The solid lines are lines of identity. Panel B: Bland Altman plots showing agreement. The solid horizontal line represents the bias or average difference while the dotted horizontal lines are 95% confidence intervals.</p

Antibody levels to SWA or SEA among children at age five years.

<p>Shown are plasma levels of antibodies to SWA (A) or SEA (B) among uninfected children (n = 390) and infected children (n = 20) at age five years. ** Wilcoxon rank-sum test p<0.001.</p

Potential of anti-CFP-10 and anti-ESAT-6 antibody/IFN-γ ratios to differentiate active and latent TB infection.

<p>Potential of anti-CFP-10 and anti-ESAT-6 antibody/IFN-γ ratios to differentiate active and latent TB infection.</p

Study participant characteristics.

<p>Study participant characteristics.</p

Effect of praziquantel treatment during pregnancy on <i>S. mansoni</i> infection status in children at age five years.

<p>Effect of praziquantel treatment during pregnancy on <i>S. mansoni</i> infection status in children at age five years.</p

CFP-10 and ESAT-6 specific antibodies in QFT-GIT nil control supernatants from APTB cases & individuals with LTBI and their discriminatory potential.

<p>Panel A: box and whisker plots showing differences in antibodies between the two groups. Panel B: Receiver operator characteristic curves showing discriminatory potential at different antibody cut-offs.</p