21 research outputs found

    Dynamic Visibility in Polygonal Scenes With the Visibility Complex

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    This paper proposes methods for e#cient maintaining of the view around a point (i.e., the visibility polygon) in static and dynamic polygonal scenes in the plane. The algorithms presented use the visibility complex, that we have adapted for polygonal scenes (composed of disjoint simple polygons) to take advantage of the spatial and temporal coherence. The first algorithm shows how to maintain the view around a moving point in O(lo

    Progress report towards meeting 2020 GSPC targets 8 & 9 in Europe - implementation and subsequent recommendations

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    As a follow-up to the European Native Seed Conservation Network (ENSCONET), funded by the European Commission between 2004 and 2009, the ENSCONET Consortium was established in 2010, and currently consists of 32 member organisations from 18 European countries that collaborate on European seed conservation and research. A comparison of the list of seed accession holdings in European seed banks as uploaded into the ENSCONET database (ENSCOBASE) with both, the most up-to-date version of the IUCN Red List and the 2011 European Red List of Vascular Plants showed a coverage of 54.8% towards GSPC target 8 1,2, far below the 75% threshold. Regarding GSPC target 9, the comparison with the Harlan and de Wet Crop Wild Relative Inventory checklist 3, the 2014 IUCN European Red List of Medicinal Plants 4 and the 1995 catalogue of the wild relatives of cultivated plants native to Europe 5 showed a coverage of 77.9%, which was above the 70% threshold. Based on this comparison, the authors of this paper, on behalf of the ENSCONET Consortium, developed recommendations and a collections strategy outlining which European threatened plant species needed to be collected as a priority if the GSPC target 8 was to be reached by 2020 2. Here we present in detail the progress made towards meeting 2020 GSPC targets 8 and 9, as well as our priority-setting method designed to guide collecting strategies by country. A key result is a country-based checklist of European threatened plant taxa to be collected and stored ex situ across the seed banks of the ENSCONET Consortium by 2020 2 . The collection plan has been shared with the Members of the ENSCONET Consortium and with the European Botanic Gardens Consortium who are encouraged to design annual seed collection programs according to this plan. The collection plan will keep being updated as new priority taxa get collected and new versions of the IUCN Red List are released. Updates of the collection plan with be shared with the Members of the Consortium and are also made available to other interested audiences so that collected taxa will - as much as possible - match the European Threatened taxa list by 2020

    High-resolution 3D imaging of whole organ after clearing: taking a new look at the zebrafish testis

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    Zebrafish testis has become a powerful model for reproductive biology of teleostean fishes and other vertebrates and encompasses multiple applications in applied and basic research. Many studies have focused on 2D images, which is time consuming and implies extrapolation of results. Three-dimensional imaging of whole organs recently became an important challenge to better understand their architecture and allow cell enumeration. Several protocols have thus been developed to enhance sample transparency, a limiting step for imaging large biological samples. However, none of these methods has been applied to the zebrafish testis. We tested five clearing protocols to determine if some of them could be applied with only small modifications to the testis. We compared clearing efficiency at both macroscopic and microscopic levels. CUBIC and PACT were suitable for an efficient transparency, an optimal optical penetration, the GFP fluorescence preservation and avoiding meaningful tissue deformation. Finally, we succeeded in whole testis 3D capture at a cellular resolution with both CUBIC and PACT, which will be valuable in a standard workflow to investigate the 3D architecture of the testis and its cellular content. This paves the way for further development of high content phenotyping studies in several fields including development, genetic or toxicology

    Détermination d'une exposition environnementale à l'amiante dans le cadre d'une démarche d'évaluation des risques sanitaires à Andouillé, Mayenne.

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    Le hameau de Rochefort, commune d'Andouillé (Mayenne), est construit autour d'une ancienne usine de transformation de l'amiante, en activité jusqu'en 1952. Sur le site industriel, réhabilité de façon artisanale, persistent des déchets amiantifères et des fibres d'amiante susceptibles d'être remises en suspension dans une zone à vocation résidentielle et touristique. La fréquentation par la population d'un tel site a amené la DDASS à s'interroger sur la nécessité de préconiser des mesures préventives, notamment afin de limiter le nombre de personnes exposées et prévenir ainsi l'apparition de pathologies liées à la présence d'amiante sur le site. De manière pragmatique se posaient donc les questions de savoir s'il était pertinent de suspendre l'urbanisation du village et de limiter l'usage de promenade autour de l'ancienne filature ? En l'état actuel du site industriel, il ne paraît pas pertinent de limiter ces usages. La présence de déchets amiantifères sur le site doit néanmoins inciter à la prudence quant à d'éventuels travaux. Si l'exposition à l'amiante à l'intérieur des bâtiments a fait l'objet de nombreux textes réglementaires et débats, on s'est jusqu'à présent peu intéressé aux expositions en atmosphère extérieure, ce qui donne un caractère original à cette approche
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