[Investigation of pseudoautosomal and bordering regions in avian Z and W chromosomes with the use of large insert genomic BAC clones] Изучение псевдоаутосомных и граничащих с ними районов Z- и W-хромосом птиц при помощи протяженных геномных BAC-клонов

Благовещенский И.Ю., Сазанова А.Л., Стекольникова В.А., Фомичев К.А., Баркова О.Ю., Романов М.Н., Сазанов А.А. Для изучения псевдоаутосомных и граничащих с ними районов Z- и W-хромосом птиц были использованы семь BAC-клонов, обнаруженных в геномных библиотеках с использованием в качестве ДНК-зондов фрагментов различных гаметологов гена ATP5А1, расположенного вблизи проксимальной границы псевдоаутосомного района половых хромосом домашней курицы и японского перепела. Методом флуоресцентной гибридизации in situ с использованием W-специфических зондов определены локализация BAC-клонов TAM31-b100C09, TAM31-b99N01, TAM31-b27P16 и TAM31-b95L18 в коротком плече Z-хромосом домашней курицы и японского перепела (район Zp23–p22) и локализация BAC-клонов CHORI–261-CH46G16, CHORI-261-CH33F10 и CHORI-261-CH64F22 на W-хромосомах этих видов птиц и в коротком плече Z-хромосом (район Zp23–p22). Различия в локализации BAC-клонов на Z- и W-хромосомах, возможно, связаны с наличием дивергенции в нуклеотидных последовательностях разных половых хромосом, находящихся за пределами псевдоаутосомного района. To study pseudoautosomal and bordering regions in the avian Z and W chromosomes, we used seven BAC clones from genomic libraries as DNA probes of fragments of different gametologs of the ATP5A1 gene located close to the proximal border of the pseudoautosomal region (PAR) of sex chromosomes of domestic chicken and Japanese quail. Localization of BAC clones TAM31-b100C09, TAM31-b99N01, TAM31-b27P16, and TAM31-b95L18 in the short arm of Z chromosomes of domestic chicken and Japanese quail (region Zp23-p22) and localization of the BAC clones CHORI-261-CH46G16, CHORI-261-CH33F10, and CHORI-261-CH64F22 on W chromosomes of these species and in the short arm of Z chromosomes (region Zp23-p22) were determined by fluorescence in situ hybridization with the use of W-specific probes. The difference in the localization of the BAC clones on the Z and W chromosomes is probably explained by divergence of the nucleotide sequences of different sex chromosomes located beyond the pseudoautosomal region

Investigation of pseudoautosomal and bordering regions in avian Z and W chromosomes with the use of large insert genomic BAC clones

To study pseudoautosomal and bordering regions in the avian Z and W chromosomes, we used seven BAC clones from genomic libraries as DNA probes of fragments of different gametologs of the ATP5A1 gene located close to the proximal border of the pseudoautosomal region (PAR) of sex chromosomes of domestic chicken and Japanese quail. Localization of BAC clones TAM31-b100C09, TAM31-b99N01, TAM31-b27P16, and TAM31-b95L18 in the short arm of Z chromosomes of domestic chicken and Japanese quail (region Zp23-p22) and localization of the BAC clones CHORI-261-CH46G16, CHORI-261-CH33F10, and CHORI-261-CH64F22 on W chromosomes of these species and in the short arm of Z chromosomes (region Zp23-p22) were determined by fluorescence in situ hybridization with the use of W-specific probes. The difference in the localization of the BAC clones on the Z and W chromosomes is probably explained by divergence of the nucleotide sequences of different sex chromosomes located beyond the pseudoautosomal region

Chromosomal localization of 15 large insert BAC clones containing three microsatellites on chicken chromosome 4 (GGA4) which refine its centromere position

Source/description: Components of shell thickness quantitative trait loci (QTL) at 53 weeks of age (ST53) and egg albumin weight QTL at 33 weeks of age (AW33) were previously shown to be linked to microsatellite markers, MCW0114 and MCW0170, respectively. Microsatellites, ADL0241 and LEI0081, were also estimated to lie close to the ST53 and AW33 QTLs, respectively, based on existing chicken genetic maps. In this paper, we report identification of 15 large insert BAC clones containing microsatellites, MCW0114, ADL0241 and MCW0170 and their localization on GGA4..