Cosmic stellar relics in the Galactic halo

We study the stellar population history and chemical evolution of the Milky Way (MW) in a hierarchical LCDM model for structure formation. Using a Monte Carlo method based on the semi-analytical EPS formalism, we reconstruct the merger tree of our Galaxy and follow the evolution of gas and stars along the hierarchy. Our approach allows us to compare the observational properties of the MW with model results, exploring different properties of primordial stars, such as their IMF and the critical metallicity for low-mass star formation, Zcr. By matching our predictions to the Metallicity Distribution Function (MDF) of metal-poor stars in the Galactic halo we find that: (i) supernova feedback is required to reproduce the observed properties of the MW; (ii) stars with [Fe/H]<-2.5 form in halos accreting Galactic Medium (GM) enriched by earlier supernova explosions; (iii) the fiducial model (Zcr=10^-4Zsun,m_PopIII=200Msun) provides an overall good fit to the MDF but cannot account for the two stars with [Fe/H]<-5; the latter can be accommodated if Zcr<10^-6Zsun but such model overpopulates the range -5.3<[Fe/H]<-4 in which no stars have been detected; (iv) the current non-detection of metal-free stars robustly constrains either Zcr>0 or the masses of the first stars m_PopIII>0.9Msun; (v) the statistical impact of second generation stars, i.e stars forming out of gas polluted only by metal-free stars, is negligible in current samples; (vi) independently of Zcr, 60% of metals in the GM are ejected through winds by halos with masses M<6x10^9 Msun, showing that low-mass halos are the dominant population contributing to cosmic metal enrichment.Comment: 18 pages, 12 figures, submitted to MNRA

From SN 2010da to NGC 300 ULX-1: Ten Years of Observations of an Unusual High Mass X-Ray Binary in NGC 300

In May 2010, an intermediate luminosity optical transient was discovered in the nearby galaxy NGC 300 by a South African amateur astronomer. In the decade since its discovery, multi-wavelength observations of the misnamed “SN 2010da” have continually reshaped our understanding of this high mass X-ray binary system. In this review, we present an overview of the multi-wavelength observations and attempt to understand the 2010 transient event, and later, the reclassification of this system as NGC 300 ULX-1: a red supergiant + neutron star ultraluminous X-ray source

Cryoprotectant-free vitrification of human spermatozoa in new artificial seminal fluid

Vitrification is a new method that has been recently introduced in Assisted Reproduction Technique programs. The aim of this study was to design a new medium similar to normal human seminal fluid (SF), formulation artificial seminal fluid (ASF), and to compare the cryoprotective potency of this medium with SF and human tubal fluid (HTF) medium. Thirty normal ejaculates were processed with the swim-up technique and sperm suspensions were divided into four aliquots: (i) fresh sample (control); (ii) vitrification in HTF medium supplemented with 5 mg/mL human serum albumin and 0.25 mol sucrose (Vit HTF); (iii) vitrification with patients' SF (Vit SF); and (iv) vitrification in ASF (Vit ASF). After warming, sperm parameters of motility, viability, and morphology were analyzed using WHO criteria. Also, sperm pellets were fixed in 2.5% glutaraldehyde and processed for scanning electron microscopy and transmission electron microscopy observations. The results showed that progressive motility (46.09 ± 10.33 vs. 36.80 ± 13.75), grade A motility (36.59 ± 11.40 vs. 16.41 ± 11.24), and normal morphology (18.74 ± 8.35 vs. 11.85 ± 5.84) and viability (68.22 ± 10.83 vs. 60.86 ± 11.72) of spermatozoa were significantly higher in Vit ASF than in Vit HTF. All parameters were better in Vit ASF than in Vit SF, but only viability was significantly different (p = 0.006). After cryopreservation, deep invagination in cytoplasm and mechanically weak point sites and folded tail were commonly observed. But, this phenomenon was more significant in Vit HTF and Vit SF than in ASF (p &lt; 0.05). In transmission electron microscopy evaluation, acrosome damage, plasma membrane loss, chromatin vacuolation, and disruption of mitochondria arrangement and structures were observed in all vitrified groups. Adherence of several tail sections together was also seen in all cryo groups. But this was seen more in Vit HTF and Vit SF than in ASF (p &lt; 0.05). In conclusion, vitrification of human spermatozoa with ASF can effectively preserve the quality of sperm motility in comparison with Vit HTF. © 2016 American Society of Andrology and European Academy of Andrology

Determinants of fibrinogen in an Italian population suffering from claudication. Lower fibrinogen in the south compared to middle and north of Italy. The ADEP Group.

Prospective studies have shown that high plasma levels of fibrinogen are independently associated with the risk of cardiovascular complications. In patients suffering from peripheral vascular disease (PVD) fibrinogen has been shown to be an independent predictor of cardiovascular disease but its determinants have never been examined in this clinical setting. DESIGN AND METHODS: Fibrinogen levels were related to clinical and laboratory variables in 2,111 patients suffering from PVD. We also analyzed whether there was a regional distribution of risk factors. RESULTS: The median values of fibrinogen was 312 mg/dL. The clinical variables examined did not differentiate patients with elevated or normal fibrinogen levels. In particular, patients with ankle/arm pressure ratio &lt; 0.8 did not show a higher prevalence of fibrinogen &gt; 312 mg/dL. Conversely, white blood cell (WBC) count and serum cholesterol levels were significantly associated with high fibrinogen levels (p &lt; 0.0001). Multiple logistic regression analysis demonstrated that areas of Italy were differently associated with high plasma fibrinogen levels (p &lt; 0.03): subjects in the north and middle of Italy having significantly higher values of fibrinogen than subjects in the south of Italy (p &lt; 0.01). A similar regional distribution was observed for WBC count and serum cholesterol levels. INTERPRETATION AND CONCLUSIONS: The regional distribution of risk factors raises the question as to whether the already reported large variability of cardiovascular events so in PVD may be attributed to a non homogeneous distribution of risk factors

Ultrastructure of cytoplasmic fragments in human cleavage stage embryos

Purpose: The goal of this study was to evaluate the ultrastructure of cytoplasmic fragments along with the effect of cytoplasmic fragment and perivitelline space coarse granulation removal (cosmetic microsurgery) from embryos before embryo transfer on ART outcomes. Methods: One hundred and fifty intracytoplasmic sperm injection cycles with male factor infertility were included in this prospective study. Patients were divided into three groups of case (n = 50), sham (n = 50), and control (n = 50). Embryos with 10–50 % fragmentation were included in this study. Cosmetic microsurgery and zona assisted hatching were only performed in case and sham groups respectively. Extracted fragments were evaluated ultrastructurally by transmission electron microscopy (TEM). Rates of clinical pregnancy, live birth, miscarriage, multiple pregnancies, and congenital anomaly in the three groups were also compared. Results: Micrographs from TEM showed that mitochondria were the most abundant structures found in the fragments along with mitochondria-vesicle complexes, Golgi apparatus, primary lysosomes, and vacuoles. There were no significant differences in demographic characteristics, laboratory and clinical data, or embryo morphological features between the groups. The rate of clinical pregnancy in control, sham, and case groups had no significant differences (24, 18, and 18 %, respectively). The rates of live birth, miscarriage, multiple pregnancy, and congenital anomaly were also similar between the different groups. Conclusions: Our data demonstrated that cosmetic microsurgery on preimplantation embryos had no beneficial effect on ART outcomes in unselected groups of patients. As mitochondria are the most abundant organelles found in cytoplasmic fragments, fragment removal should be performed with more caution in embryos with moderate fragmentation