Field efficacy of Bacillus thuringiensis galleriae strain SDS-502 for the management of alfalfa weevil and its impact on Bathyplectes spp. parasitization rate

Alfalfa weevil, Hypera postica Gyllenhal, is an important pest in forage alfalfa worldwide, and especially so on the Northern Plains of North America. Neither the weevil-specific fungus, Erynia phytonomi, nor the weevil\u27s parasitoids are able to routinely suppress outbreaks as they do in the eastern U.S. A new Bacillus thuringiensis var. galleriae, having a Cry8Da coleopteran-active toxin, has been recently commercialized. We examined the efficacy of this B. thuringiensis product against the H. postica in replicated field trials in north central Montana. Because it has been suggested that efficiency of the parasitoids, Bathyplectes curculionis and Oomyzus incertus, was inversely proportional to host numbers (i.e., parasitoid efficiency increased when host population is low), we also sought to determine if a partial reduction of larval H. postica populations with a B. thuringiensis would yield to greater parasitoid efficiency, manifested as higher percent parasitism among the surviving larvae. The B. thuringiensis gave 27–40% reduction in weevil numbers at the low label rate, 55–59% for the high label rate. Mean parasitism at the two research locations varied from 5–26% and 17–36% respectively, but application of the B. thuringiensis had no significant effect on parasitism levels, i.e. parasitism was not greater in treated than in carrier control plots

Cover crop and conidia delivery system impacts on soil persistence of \u3ci\u3eMetarhizium anisopliae\u3c/i\u3e (Hypocreales: Clavicipitaceae) in sugarbeet

The sugarbeet root maggot, Tetanops myopaeformis (Röder), is a major North American pest of sugarbeet, Beta vulgaris L. Previous research suggests that moderate T. myopaeformis control is possible with the entomopathogen Metarhizium anisopliae (Metch.) Sorok. We conducted a three-year (2002‒2004) experiment to assess impacts of oat, Avena sativa L. and rye, Secale cereale L., cover crops on persistence of corn grit-based granular or spray formulations of M. anisopliae isolate ATCC 62176 (i.e. MA 1200) applied at 8 x 1012 viable conidia/ha in sugarbeet. More colony forming units (CFUs) were detected immediately after application [0 days after treatment (DAT)] in spray plots than granule-treated plots. However, 76‒92% declines in CFUs per gram of soil occurred in spray plots within 30 DAT. Substantially (i.e. 83‒560%) more rainfall occurred in June 2002 than during June of any other year. Subsequently, 71‒670% increases in CFU concentrations occurred by 60 DAT in M. anisopliae granuletreated plots with oat or rye cover crops that year. CFU density increases were higher in cover crops in 2002, but no significant cover crop effects were detected. Conidia persisted for up to 30 DAT in M. anisopliae spray plots and 60 DAT in granule-treated plots in 2002; however, no increases occurred in the years with less June rainfall. Trends suggest that M. anisopliae aqueous sprays result in greater conidia concentrations than granules at sugarbeet plant bases in June during T. myopaeformis oviposition and larval establishment on host plants. Increases are possible when delivering conidia via granules, but high post-application rainfall could be necessary for conidia production

Immune Response of Mormon Crickets That Survived Infection by Beauveria bassiana

Beauveria bassiana (Fungi: Ascomycota) is an entomopathogenic fungus that serves as a biological control agent of Mormon crickets Anabrus simplex Haldeman (Orthoptera: Tettigoniidae) and other grasshopper pests. To measure the dose-dependent response of Mormon crickets to fungal attack, we applied B. bassiana strain GHA topically to adults using doses of 5.13 × 104 to 1.75 × 106 conidia in sunflower oil, with oil only as a control. After three weeks, we assessed the survivors' hemolymph for fungal cells, active phenoloxidase (PO), and lysozyme. Mortality increased and body mass of survivors decreased with conidial dose. survivors' PO activity was elevated to the same level independent of dose. Those with fungal cells visible in their hemolymph did not differ in PO activity from those with clear hemolymph. We conclude that circulating PO may be an important enzymatic defense against Beauveria infection and that it is associated with attempted clearing of Beauveria blastospores and hyphae from Mormon cricket hemolymph

Cover crop and conidia delivery system impacts on soil persistence of \u3ci\u3eMetarhizium anisopliae\u3c/i\u3e (Hypocreales: Clavicipitaceae) in sugarbeet

The sugarbeet root maggot, Tetanops myopaeformis (Röder), is a major North American pest of sugarbeet, Beta vulgaris L. Previous research suggests that moderate T. myopaeformis control is possible with the entomopathogen Metarhizium anisopliae (Metch.) Sorok. We conducted a three-year (2002‒2004) experiment to assess impacts of oat, Avena sativa L. and rye, Secale cereale L., cover crops on persistence of corn grit-based granular or spray formulations of M. anisopliae isolate ATCC 62176 (i.e. MA 1200) applied at 8 x 1012 viable conidia/ha in sugarbeet. More colony forming units (CFUs) were detected immediately after application [0 days after treatment (DAT)] in spray plots than granule-treated plots. However, 76‒92% declines in CFUs per gram of soil occurred in spray plots within 30 DAT. Substantially (i.e. 83‒560%) more rainfall occurred in June 2002 than during June of any other year. Subsequently, 71‒670% increases in CFU concentrations occurred by 60 DAT in M. anisopliae granuletreated plots with oat or rye cover crops that year. CFU density increases were higher in cover crops in 2002, but no significant cover crop effects were detected. Conidia persisted for up to 30 DAT in M. anisopliae spray plots and 60 DAT in granule-treated plots in 2002; however, no increases occurred in the years with less June rainfall. Trends suggest that M. anisopliae aqueous sprays result in greater conidia concentrations than granules at sugarbeet plant bases in June during T. myopaeformis oviposition and larval establishment on host plants. Increases are possible when delivering conidia via granules, but high post-application rainfall could be necessary for conidia production

Evaluation of the effectiveness of entomopathogens for the management of wireworms (Coleoptera: Elateridae) on spring wheat

Wireworms, the larval stage of click beetles (Coleoptera: Elateridae), are serious soil dwelling pests of small grains, corn, sugar beets, and potatoes. Limonius californicus and Hypnoidus bicolor are the predominant wireworm species infesting wheat in Montana, particularly in the \u27Golden Triangle\u27 area of north-central Montana. Wireworm populations in field crops are increasing, but currently available insecticides provide only partial control, and no alternative management tools exist. In our study, three entomopathogenic fungi were tested for their efficacy against wireworms in spring wheat at two field locations (Ledger and Conrad, Montana, USA) in 2013. The three fungi (Metarhizium brunneum F52, Beauveria bassiana GHA, and Metarhizium robertsii DWR 346) were evaluated as seed-coat, in-furrow granular, and soil band-over-row drench applications in addition to imidacloprid (Gaucho® 600) seed treatment (as a chemical check), the approach currently being used by growers. Wireworm damage in these treatments was evaluated as standing plant counts, wireworm population surveys, and yield. The three fungi, applied as formulated granules or soil drenches, and the imidacloprid seed treatment all resulted in significantly higher plant stand counts and yields at both locations than the fungus-coated seed treatments or the untreated control. Significant differences were detected among the application methods but not among the species of fungi within each application method. All three fungi, when applied as granules in furrow or as soil drenches, were more effective than when used as seed-coating treatments for wireworm control, and provided an efficacy comparable or superior to imidacloprid. The fungi used in this study provided significant plant and yield protection under moderate wireworm pressure, supporting their value in the management of this pest. © 2014 The Authors

Laboratory bioassays of entomopathogenic fungi for control of Delia radicum (L.) larvae

Laboratory soil bioassays were performed at economic Weld rates for in-furrow (3.85 x 106 spores/g dry soil) and broadcast (3.85 x 105 spores/g dry soil) applications with three isolates of Metarhizium anisopliae (F52, ATCC62176, and ARSEF5520) and one isolate of Beauveria bassiana (GHA). All isolates tested were infective to second instar Delia radicum (L.). The conditionally registered M. anisopliae isolate (F52) performed best killing an average of 85 and 72% of D. radicum larvae at the high and low concentration, respectively. The mean LC50 and LC95 of F52 against second instar D. radicum was 2.7 x 106 and 1.8 x 108 spores/g dry soil, respectively. The use of F52 in an integrated management program is discussed

Late Season Biological Control of Whiteflies in Fall Cantaloupe Using Formulations of Beauveria Bassiana

Beauveria bassiana is a naturally occurring fungal disease of insects that has been shown to be an effective biological control against whiteflies in cotton and vegetable crops. Six treatments were initiated in drip irrigated fall cantaloupe on October 2, and repeated on October 9 and 23. The six treatments consisted of 1) a check or unsprayed plot; 2) 0.5 lb. Mycotrol WP/acre; 3) 1 Ib. Mycotrol WP /acre; 4) 1 pt. Mycotrol ES/acre; 5) 0.5 lb. Mycotrol WP /acre + pyrethroid tank mix; and 6) 12 oz. Naturalis-L/acre. Under moderate to light sweetpotato whitefly pressure, the Mycotrol formulations provided significant control (68-79%) compared to unsprayed check plots, and were superior to Naturalis-L formulation whose effects were relatively short lived. Mycotrol WP applied in three applications at the labeled rate of 1 lb. product/acre had the cumulative effect of maintaining adult whitefly leaf counts below the currently recommended economic threshold of 3 per leaf at 28 days after treatment initiation, under the conditions of this study