Morphological and functional diversification during kelp evolution

Kelps are highly successful ecosystem engineers that substantially increase the productivity of nearshore ecosystems, forming nursery habitat for other species. Despite the importance of kelps to the modern ecology of temperate ecosystems, we have a limited understanding of their evolutionary relationships, the diversification dynamics that led to their modern distributions, and the factors that determine where they are found in nature. In this dissertation, I quantitatively explore how distributions and modern ecological strategies have arisen through kelp evolution. I utilize phylogenomic approaches to determine the evolutionary relationships between kelp species and the timing of their diversification, elucidating many previously unknown phylogenetic relationships between kelps (Ch. 2). I then investigate the strategies that kelps use to survive on wave-swept shores and test for trade-offs that may have constrained their broad range of morphological variation. I use flow tank experiments and field mechanical testing to investigate how external forces from the environment interact with morphology. I show that kelps with high mechanical support also experience greater environmental forces than weaker, more streamlined species, consistent with well known trade-offs of stress resistance in other organisms (Ch. 3). I also investigate the interspecific scaling of biomass allocation in organs of kelp that are analogous to those of land plants and showed that there are shared features of how size influences morphology in both groups (Ch. 4). Lastly, I combine trait and phylogenetic information to explore patterns of trait evolution and determine how species that specialize in different environments are distributed across the phylogeny. I assess whether phylogenetic relatedness or trait differences explain the assembly of kelp communities and demonstrate that kelp communities are composed of distantly related species that have converged on similar traits (Ch. 5). Taken together, these studies offer a multifaceted perspective on the morphological and ecological diversification of kelps and demonstrate that ecological strategies are convergent among phylogenetically divergent lineages.Science, Faculty ofBotany, Department ofGraduat

Validating Landsat Analysis Ready Data for Nearshore Sea Surface Temperature Monitoring in the Northeast Pacific

In the face of global ocean warming, monitoring essential climate variables from space is necessary for understanding regional trends in ocean dynamics and their subsequent impacts on ecosystem health. Analysis Ready Data (ARD), being preprocessed satellite-derived products such as Sea Surface Temperature (SST), allow for easy synoptic analysis of temperature conditions given the consideration of regional biases within a dynamic range. This is especially true for SST retrieval in thermally complex coastal zones. In this study, we assessed the accuracy of 30 m resolution Landsat ARD Surface Temperature products to measure nearshore SST, derived from Landsat 8 TIRS, Landsat 7 ETM+, and Landsat 5 TM thermal bands over a 37-year period (1984–2021). We used in situ lighthouse and buoy matchup data provided by Fisheries and Oceans Canada (DFO). Excellent agreement (R2 of 0.94) was found between Landsat and spring/summer in situ SST at the farshore buoy site (>10 km from the coast), with a Landsat mean bias (root mean square error) of 0.12 °C (0.95 °C) and a general pattern of SST underestimation by Landsat 5 of −0.28 °C (0.96 °C) and overestimation by Landsat 8 of 0.65 °C (0.98 °C). Spring/summer nearshore matchups revealed the best Landsat mean bias (root mean square error) of −0.57 °C (1.75 °C) at 90–180 m from the coast for ocean temperatures between 5 °C and 25 °C. Overall, the nearshore image sampling distance recommended in this manuscript seeks to capture true SST as close as possible to the coastal margin—and the critical habitats of interest—while minimizing the impacts of pixel mixing and adjacent land emissivity on satellite-derived SST.Science, Irving K. Barber Faculty of (Okanagan)Non UBCBiology, Department of (Okanagan)ReviewedFacultyResearche

Validating Landsat Analysis Ready Data for Nearshore Sea Surface Temperature Monitoring in the Northeast Pacific

In the face of global ocean warming, monitoring essential climate variables from space is necessary for understanding regional trends in ocean dynamics and their subsequent impacts on ecosystem health. Analysis Ready Data (ARD), being preprocessed satellite-derived products such as Sea Surface Temperature (SST), allow for easy synoptic analysis of temperature conditions given the consideration of regional biases within a dynamic range. This is especially true for SST retrieval in thermally complex coastal zones. In this study, we assessed the accuracy of 30 m resolution Landsat ARD Surface Temperature products to measure nearshore SST, derived from Landsat 8 TIRS, Landsat 7 ETM+, and Landsat 5 TM thermal bands over a 37-year period (1984–2021). We used in situ lighthouse and buoy matchup data provided by Fisheries and Oceans Canada (DFO). Excellent agreement (R2 of 0.94) was found between Landsat and spring/summer in situ SST at the farshore buoy site (>10 km from the coast), with a Landsat mean bias (root mean square error) of 0.12 °C (0.95 °C) and a general pattern of SST underestimation by Landsat 5 of −0.28 °C (0.96 °C) and overestimation by Landsat 8 of 0.65 °C (0.98 °C). Spring/summer nearshore matchups revealed the best Landsat mean bias (root mean square error) of −0.57 °C (1.75 °C) at 90–180 m from the coast for ocean temperatures between 5 °C and 25 °C. Overall, the nearshore image sampling distance recommended in this manuscript seeks to capture true SST as close as possible to the coastal margin—and the critical habitats of interest—while minimizing the impacts of pixel mixing and adjacent land emissivity on satellite-derived SST

Cell wall chemistry and tissue structure underlie shifts in material properties of a perennial kelp

<p><i>Laminaria</i> is an abundant kelp genus in temperate nearshore ecosystems that grows with a circannual ‘stop-start’ pattern. Species of <i>Laminaria</i> play important ecological roles in kelp forests worldwide and are harvested commercially as a source of food and valuable extracts. In order to evaluate seasonal differences in tissue properties and composition, we compared the material properties, histology and cell-wall composition of overwintering blades with newly synthesized, actively growing blades from <i>Laminaria setchellii</i>. We found that overwintering blades were fortified with a thicker cortex and increased cell wall investment, leading to increased material strength. Overwintering tissues were composed of higher proportions of cellulose and fucose-containing polysaccharides (i.e. FCSPs, fucoidans) than newly formed blades and were found to possess thicker cell walls, likely to withstand the waves of winter storms. Chemical cell wall profiling revealed that significant proportions of fucose were associated with cellulose, especially in overwintering tissues, confirming the association between cellulose and some fucose-containing polysaccharides. Changes in material properties during the resting phase may allow these kelps to retain their non-growing blades through several months of winter storms. The results of this study demonstrate how one species might regulate its material properties seasonally, and at the same time shed light on the mechanisms that might control the material properties of kelps in general.</p

Marine heatwaves threaten cryptic coral diversity and erode associations among coevolving partners

Climate change–amplified marine heatwaves can drive extensive mortality in foundation species. However, a paucity of longitudinal genomic datasets has impeded understanding of how these rapid selection events alter cryptic genetic structure. Heatwave impacts may be exacerbated in species that engage in obligate symbioses, where the genetics of multiple coevolving taxa may be affected. Here, we tracked the symbiotic associations of reef-building corals for 6 years through a prolonged heatwave, including known survivorship for 79 of 315 colonies. Coral genetics strongly predicted survival of the ubiquitous coral, Porites (massive growth form), with variable survival (15 to 61%) across three morphologically indistinguishable—but genetically distinct—lineages. The heatwave also disrupted strong associations between these coral lineages and their algal symbionts (family Symbiodiniaceae), with symbiotic turnover in some colonies, resulting in reduced specificity across lineages. These results highlight how heatwaves can threaten cryptic genotypes and decouple otherwise tightly coevolved relationships between hosts and symbionts. Marine heatwaves differentially impact cryptic coral lineages and decouple tight relationships between host and symbiont

Dynamic symbioses reveal pathways to coral survival through prolonged heatwaves

Prospects for coral persistence through increasingly frequent and extended heatwaves seem bleak. Coral recovery from bleaching is only known to occur after temperatures return to normal, and mitigation of local stressors does not appear to augment coral survival. Capitalizing on a natural experiment in the equatorial Pacific, we track individual coral colonies at sites spanning a gradient of local anthropogenic disturbance through a tropical heatwave of unprecedented duration. Unexpectedly, some corals survived the event by recovering from bleaching while still at elevated temperatures. These corals initially had heat-sensitive algal symbiont communities, endured bleaching, and then recovered through proliferation of heat-tolerant symbionts. This pathway to survival only occurred in the absence of strong local stressors. In contrast, corals in highly disturbed areas were already dominated by heat-tolerant symbionts, and despite initially resisting bleaching, these corals had no survival advantage in one species and 3.3 times lower survival in the other. These unanticipated connections between disturbance, coral symbioses and heat stress resilience reveal multiple pathways to coral survival through future prolonged heatwaves

Environmental heterogeneity mediates scale-dependent declines in kelp diversity on intertidal rocky shores.

Biodiversity loss is driven by interacting factors operating at different spatial scales. Yet, there remains uncertainty as to how fine-scale environmental conditions mediate biological responses to broad-scale stressors. We surveyed intertidal rocky shore kelp beds situated across a local gradient of wave action and evaluated changes in kelp diversity and abundance after more than two decades of broad scale stressors, most notably the 2013-2016 heat wave. Across all sites, species were less abundant on average in 2017 and 2018 than during 1993-1995 but changes in kelp diversity were dependent on wave exposure, with wave exposed habitats remaining stable and wave sheltered habitats experiencing near complete losses of kelp diversity. In this way, wave exposed sites have acted as refugia, maintaining regional kelp diversity despite widespread local declines. Fucoids, seagrasses and two stress-tolerant kelp species (Saccharina sessilis, Egregia menziesii) did not decline as observed in other kelps, and the invasive species Sargassum muticum increased significantly at wave sheltered sites. Long-term monitoring data from a centrally-located moderate site suggest that kelp communities were negatively impacted by the recent heatwave which may have driven observed losses throughout the region. Wave-sheltered shores, which saw the largest declines, are a very common habitat type in the Northeast Pacific and may be especially sensitive to losses in kelp diversity and abundance, with potential consequences for coastal productivity. Our findings highlight the importance of fine-scale environmental heterogeneity in mediating biological responses and demonstrate how incorporating differences between habitat patches can be essential to capturing scale-dependent biodiversity loss across the landscape

Whole genome population structure of North Atlantic kelp confirms high-latitude glacial refugia

Coastal refugia during the Last Glacial Maximum (~21,000 years ago) have been hypothesized at high latitudes in the North Atlantic, suggesting marine populations persisted through cycles of glaciation and are potentially adapted to local environments. Here, whole-genome sequencing was used to test whether North Atlantic marine coastal populations of the kelp Alaria esculenta survived in the area of southwestern Greenland during the Last Glacial Maximum. We present the first annotated genome for A. esculenta and call variant positions in 54 individuals from populations in Atlantic Canada, Greenland, Faroe Islands, Norway and Ireland. Differentiation across populations was reflected in ~1.9 million single nucleotide polymorphisms, which further revealed mixed ancestry in the Faroe Islands individuals between putative Greenlandic and European lineages. Time-calibrated organellar phylogenies suggested Greenlandic populations were established during the last interglacial period more than 100,000 years ago, and that the Faroe Islands population was probably established following the Last Glacial Maximum. Patterns in population statistics, including nucleotide diversity, minor allele frequencies, heterozygosity and linkage disequilibrium decay, nonetheless suggested glaciation reduced Canadian Atlantic and Greenlandic populations to small effective sizes during the most recent glaciation. Functional differentiation was further reflected in exon read coverage, which revealed expansions unique to Greenland in 337 exons representing 162 genes, and a modest degree of exon loss (103 exons from 56 genes). Altogether, our genomic results provide strong evidence that A. esculenta populations were resilient to past climatic fluctuations related to glaciations and that high-latitude populations are potentially already adapted to local conditions as a result.publishedVersio