Identification and molecular characterization of Chryseobacterium vrystaatense ST1 isolated from oligomineral water of southeast Serbia

The isolation and molecular characterization of bacterial strains isolated from water sources in the Vlasina Mountain in southeast Serbia, confirmed the presence of a new species Chryseobacterium vrystaatense ST1. This Gram- negative species showed an extremely low level of biochemical reactivity in biochemical tests. The gene for 16S rRNA was amplified by PCR using universal primers and sequenced. Comparison of 16S rRNA gene sequence and phenotypic features indicated that the isolate ST belonged to Chryseobacterium vrystaatense. A BLAST search of sequenced 1088 nucleotides of the 16S rRNA gene with all sequences deposited in the NCBI collection showed the highest similarity (98%) with the strain Chryseobacterium vrystaatense sp. nov., designated as strain R-23533. The very high homology of these two strains allowed classification of our strain at the species level, but some differences indicate, and indirectly confirm, that the isolate ST is an authentic representative. On the basis of these results, we could conclude that Chryseobacterium vrystaatense ST was for first time isolated in Serbia, which is particularly important when one bears in mind that there are only three sequences of this species deposited in the NCBI collection

The biological activities of roots and aerial parts of Alchemilla vulgaris L

The phytochemical composition, in vitro antioxidant and antimicrobial activities, cytotoxicity and antigenotoxicity of fruit extracts of Opuntia dillenii were studied. The phytochemical composition was evaluated using HPLC, GC-MS and UV–Vis spectrophotometry. Spectrophotometrical methods were used to estimate the antioxidant potential. Antimicrobial activity was determined using a microdilution method. The cytotoxic effects of the extracts were evaluated using the MTT assay. In vitro DNA-protective activity against hydroxyl radicalinduced DNA damage was also determined. The results showed that polar extracts of O. dillenii had a significant amount of phenolic compounds, including flavonoids, whereas non-polar extracts had mostly terpenoids and fatty acid derivatives. Moreover, several extracts showed good antioxidant and antimicrobial activities, with low cytotoxicity and significant DNA-protective effects. These results showed that the extracts of O. dillenii have promising bioactivity and further studies on the potential application in different areas of food and health might be beneficial

HOW CAN SMALL-SCALE EVENTS CONTRIBUTE to the TOURISM PROGRESS of AN UNDEVELOPED BORDER AREA? LESSON from EASTERN SERBIA

The study analyses the role and impact of the event "Danube Fair" (Eastern Serbia) in the tourism progress of the less-advantaged border area. The aim of this paper is to emphasize the significance of the role of events in the tourism development of rural settings and the attraction of domestic and foreign visitors with the specific offer of this part of Serbia. The tourism valorization of the event “Danube Fair” was done according to the geographical and economic criteria, and it was determined that it belongs to the category of regional events. In the paper, special attention was paid to the analysis of tourists’ satisfaction with this event as a geographical criterion, especially in dependence with the visitors’ origin, since it is what the positioning of an event on the tourism market depends on, both on the local and on the international level. “Danube Fair” presents the example of a relatively small-scale event, which can become a basis of tourism growth, with proper valorization and affirmation of local products and services, and their introduction to the international tourism market. © 2020 Editura Universitatii din Oradea. All rights reserved.17-22-07001-ОГНAuthors would like to thank Russian Foundation for basic research for the financial support provided within the project No. 17-22-07001-ОГН (Complex algorithm for cultural regeneration of minor industrial cities within the context of agglomeration processes in Russia and Europe)

The effects of colloidal SiO2 and inhibitor on the solid deposit formation in geothermal water of low hardness

Low solubility of SiO2 and its occurrence in geothermal waters in the form of ionic, colloidal and suspended state are the main cause of the solid deposit occurrence. Certain chemical types of silica, under the influence of Fe2+, Al3+, F−, OH- ions and other micro-constituents, and due to significantly decreased solubility of SiO2, stimulate nucleation, particle growth and solid deposit formation. The aim of this paper is to inhibit the process of nucleation and solid deposit formation by adding originally designed inhibitor in the form of an emulsion, when the total concentration of the present and added colloidal SiO2 is beyond the solubility limit (120 mg/dm3). By turbidimetric, SEM, EDS and XRD analysis, the processes of solid deposit formation were investigated in Vranjska Banja (Serbia) spa geothermal water source (water hardness of 4ºdH), and theoretical and practical conclusions were made

Geographical and biological analysis of the water quality of Moravica spring in the Sokobanjska Moravica drainage basin, Serbia

In this work we performed a geographical analysis of the Moravica spring locality in the Sokobanjska Moravica drainage basin in Serbia, as well as an analysis of the physical, chemical, and biological parameters of the water during a one-year period. The basic sanitary characteristics and physical, chemical, and biological parameters, necessary for understanding locality conditions, were studied, and the saprobity index, class of quality, O/H index, degree of saprobity, degree of trophicity, and category based on the phosphatase activity index (PAI) were determined. Our results point to the need for continual monitoring of the water quality in the spring locality

VSCAN: An Enhanced Video Summarization using Density-based Spatial Clustering

In this paper, we present VSCAN, a novel approach for generating static video summaries. This approach is based on a modified DBSCAN clustering algorithm to summarize the video content utilizing both color and texture features of the video frames. The paper also introduces an enhanced evaluation method that depends on color and texture features. Video Summaries generated by VSCAN are compared with summaries generated by other approaches found in the literature and those created by users. Experimental results indicate that the video summaries generated by VSCAN have a higher quality than those generated by other approaches.Comment: arXiv admin note: substantial text overlap with arXiv:1401.3590 by other authors without attributio

Dropwort (Filipendula hexapetala Gilib.): potential role as antioxidant and antimicrobial agent

The aim of this study was to investigate the antioxidant activity of the methanolic extracts of Filipendula hexapetala Gilib. aerial parts (FHA) and roots (FHR) and their potential in different model systems, as well as antimicrobial activity. According to this, a number of assays were employed to evaluate the antioxidant and antimicrobial potential of F. hexapetala extracts. In addition, the antioxidant activity assays in different model systems were carried out, as well as pH, thermal and gastrointestinal stability studies. The phenolic compounds contents in FHA and FHR were also determined. The results showed that F. hexapetala extracts had considerable antioxidant activity in vitro and a great stability in different conditions. The extracts exhibited antimicrobial activity against most of the tested bacterial and fungal species. Also, the extracts contain high level of phenolic compounds, especially aerial parts extract

Pathogenicity and diversity of vegetative compatibility of Fusarium verticillioides

Pathogenicity of 10 Fusarium verticillioides isolates, originated from grain of wheat (five isolates) and maize (five isolates), were studied under greenhouse conditions. Based on different parameters of the pathogenicity estimate (a scale for % of nonemerged plants, % of survived plants, plant vigour - the growth and dry weight of roots and epicotyls and disease severity) it was determined that all F. verticillioides isolates expressed a different degree of pathogenicity. According to % of nonemerged plants six three and one F. verticillioides isolates expressed low, moderate and high degree of pathogenicity, respectively. All F. verticillioides isolates reduced the plant survival rate and vigour, while the disease severity ranged from 2.0 to 3.54. Two types of nit mutants, nit1 and NitM, were obtained by the use of the method of vegetative compatibility. The frequency of nit1 mutants was greater (58.79%) than the frequency of NitM mutants (5.77%). A total of 10 vegetative compatibility groups (VCGs) of F. verticillioides were established in the complementation tests. These results point out to a high genetic diversity of F. verticillioides population