Surface Fluorescence Studies of Tissue Mitochondrial Redox State in Isolated Perfused Rat Lungs

We designed a fiber-optic-based optoelectronic fluorometer to measure emitted fluorescence from the auto-fluorescent electron carriers NADH and FAD of the mitochondrial electron transport chain (ETC). The ratio of NADH to FAD is called the redox ratio (RR = NADH/FAD) and is an indicator of the oxidoreductive state of tissue. We evaluated the fluorometer by measuring the fluorescence intensities of NADH and FAD at the surface of isolated, perfused rat lungs. Alterations of lung mitochondrial metabolic state were achieved by the addition of rotenone (complex I inhibitor), potassium cyanide (KCN, complex IV inhibitor) and/or pentachlorophenol (PCP, uncoupler) into the perfusate recirculating through the lung. Rotenone- or KCN-containing perfusate increased RR by 21 and 30%, respectively. In contrast, PCP-containing perfusate decreased RR by 27%. These changes are consistent with the established effects of rotenone, KCN, and PCP on the redox status of the ETC. Addition of blood to perfusate quenched NADH and FAD signal, but had no effect on RR. This study demonstrates the capacity of fluorometry to detect a change in mitochondrial redox state in isolated perfused lungs, and suggests the potential of fluorometry for use in in vivo experiments to extract a sensitive measure of lung tissue health in real-time

The First Public Release of South Pole Telescope Data: Maps of a 95 deg^2 Field from 2008 Observations

The South Pole Telescope (SPT) has nearly completed a 2500 deg^2 survey of the southern sky in three frequency bands. Here, we present the first public release of SPT maps and associated data products. We present arcminute-resolution maps at 150 GHz and 220 GHz of an approximately 95 deg^2 field centered at R.A. 82°.7, decl. –55°. The field was observed to a depth of approximately 17 μK arcmin at 150 GHz and 41 μK arcmin at 220 GHz during the 2008 austral winter season. Two variations on map filtering and map projection are presented, one tailored for producing catalogs of galaxy clusters detected through their Sunyaev-Zel'dovich effect signature and one tailored for producing catalogs of emissive sources. We describe the data processing pipeline, and we present instrument response functions, filter transfer functions, and map noise properties. All data products described in this paper are available for download at http://pole.uchicago.edu/public/data/maps/ra5h30dec-55 and from the NASA Legacy Archive for Microwave Background Data Analysis server. This is the first step in the eventual release of data from the full 2500 deg^2 SPT survey

Optical Imaging of Lipopolysaccharide-induced Oxidative Stress in Acute Lung Injury from Hyperoxia and Sepsis

Reactive oxygen species (ROS) have been implicated in the pathogenesis of many acute and chronic pulmonary disorders such as acute lung injury (ALI) in adults and bronchopulmonary dysplasia (BPD) in premature infants. Bacterial infection and oxygen toxicity, which result in pulmonary vascular endothelial injury, contribute to impaired vascular growth and alveolar simplification seen in the lungs of premature infants with BPD. Hyperoxia induces ALI, reduces cell proliferation, causes DNA damage and promotes cell death by causing mitochondrial dysfunction. The objective of this study was to use an optical imaging technique to evaluate the variations in fluorescence intensities of the auto-fluorescent mitochondrial metabolic coenzymes, NADH and FAD in four different groups of rats. The ratio of these fluorescence signals (NADH/FAD), referred to as NADH redox ratio (NADH RR) has been used as an indicator of tissue metabolism in injuries. Here, we investigated whether the changes in metabolic state can be used as a marker of oxidative stress caused by hyperoxia and bacterial lipopolysaccharide (LPS) exposure in neonatal rat lungs. We examined the tissue redox states of lungs from four groups of rat pups: normoxic (21% O2) pups, hyperoxic (90% O2) pups, pups treated with LPS (normoxic + LPS), and pups treated with LPS and hyperoxia (hyperoxic + LPS). Our results show that hyperoxia oxidized the respiratory chain as reflected by a ~ 31% decrease in lung tissue NADH RR as compared to that for normoxic lungs. LPS treatment alone or with hyperoxia had no significant effect on lung tissue NADH RR as compared to that for normoxic or hyperoxic lungs, respectively. Thus, NADH RR serves as a quantitative marker of oxidative stress level in lung injury caused by two clinically important conditions: hyperoxia and LPS exposure

Physical Properties of Four SZE-Selected Galaxy Clusters in the Southern Cosmology Survey

We present the optical and X-ray properties of four clusters recently discovered by the South Pole Telescope (SPT) using the Sunyaev-Zel'dovich effect (SZE). The four clusters are located in one of the common survey areas of the southern sky that is also being targeted by the Atacama Cosmology Telescope (ACT) and imaged by the CTIO Blanco 4-m telescope. Based on publicly available griz optical images and XMM-Newton and ROSAT X-ray observations we analyse the physical properties of these clusters and obtain photometric redshifts, luminosities, richness and mass estimates. Each cluster contains a central elliptical whose luminosity is consistent with SDSS cluster studies. Our mass estimates are well above the nominal detection limit of SPT and ACT; the new SZE clusters are very likely massive systems with M>~5x10^14 M_sun.Comment: 5 pages, 2 figures. ApJL accepte

Southern Cosmology Survey III: QSO's from Combined GALEX and Optical Photometry

We present catalogs of QSO candidates selected using photometry from GALEX combined with SDSS in the Stripe 82 region and Blanco Cosmology Survey (BCS) near declination -55 degrees. The SDSS region contains ~700 objects with magnitude i < 20 and ~3600 objects with i < 21.5 in a ~60 square degree sky region, while the BCS region contains ~280 objects with magnitude i < 20 and ~2000 objects with i < 21.5 for a 11 square degree sky region that is being observed by three current microwave Sunyaev-Zeldovich surveys. Our QSO catalog is the first one in the BCS region. Deep GALEX exposures (~2000 seconds in FUV and NUV, except in three fields) provide high signal-to-noise photometry in the GALEX bands (FUV, NUV < 24.5 mag). From this data, we select QSO candidates using only GALEX and optical r-band photometry, using the method given by Atlee and Gould (2008). In the Stripe 82 field, 60% (30%) of the GALEX selected QSO's with optical magnitude i<20 (i<21.5) also appear in the Richards et al. (2008) QSO catalog constructed using 5-band optical SDSS photometry. Comparison with the same catalog by Richards et al. shows that the completeness of the sample is approximately 40%(25%). However, for regions of the sky with very low dust extinction, like the BCS 23hr field and the Stripe 82 between 0 and 10 degrees in RA, our completeness is close to 95%, demonstrating that deep GALEX observations are almost as efficient as multi-wavelength observations at finding QSO's. GALEX observations thus provide a viable alternate route to QSO catalogs in sky regions where u-band optical photometry is not available. The full catalog is available at http://www.ice.csic.es/personal/jimenez/PHOTOZComment: Submitted to ApJ

Comparison of Hectospec Virial Masses with SZE Measurements

We present the first comparison of virial masses of galaxy clusters with their Sunyaev-Zel'dovich Effect (SZE) signals. We study 15 clusters from the Hectospec Cluster Survey (HeCS) with MMT/Hectospec spectroscopy and published SZE signals. We measure virial masses of these clusters from an average of 90 member redshifts inside the radius $r_{100}$. The virial masses of the clusters are strongly correlated with their SZE signals (at the 99% confidence level using a Spearman rank-sum test). This correlation suggests that $Y_{SZ}$ can be used as a measure of virial mass. Simulations predict a powerlaw scaling of $Y_{SZ}\propto M_{200}^\alpha$ with $\alpha\approx$1.6. Observationally, we find $\alpha$=1.11$\pm$0.16, significantly shallower (given the formal uncertainty) than the theoretical prediction. However, the selection function of our sample is unknown and a bias against less massive clusters cannot be excluded (such a selection bias could artificially flatten the slope). Moreover, our sample indicates that the relation between velocity dispersion (or virial mass estimate) and SZE signal has significant intrinsic scatter, comparable to the range of our current sample. More detailed studies of scaling relations are therefore needed to derive a robust determination of the relation between cluster mass and SZE.Comment: 6 pages, 2 figures, accepted to ApJ Letters, minor revisions, shortened titl

XMM-Newton detection of two clusters of galaxies with strong SPT Sunyaev-Zel'dovich effect signatures

We report on the discovery of two galaxy clusters, SPT-CL J2332-5358 and SPT-CL J2342-5411, in X-rays. These clusters were also independently detected through their Sunyaev-Zel'dovich effect by the South Pole Telescope, and confirmed in the optical band by the Blanco Cosmology Survey. They are thus the first clusters detected under survey conditions by all major cluster search approaches. The X-ray detection is made within the frame of the XMM-BCS cluster survey utilizing a novel XMM-Newton mosaic mode of observations. The present study makes the first scientific use of this operation mode. We estimate the X-ray spectroscopic temperature of SPT-CL J2332-5358 (at redshift z=0.32) to T = 9.3 (+3.3/-1.9) keV, implying a high mass, M_{500} = 8.8 +/- 3.8 \times 10^{14} M_{sun}. For SPT-CL J2342-5411, at z=1.08, the available X-ray data doesn't allow us to directly estimate the temperature with good confidence. However, using our measured luminosity and scaling relations we estimate that T = 4.5 +/- 1.3 keV and M_{500} = 1.9 +/- 0.8 \times 10^{14} M_{sun}. We find a good agreement between the X-ray masses and those estimated from the Sunyaev-Zel'dovich effect.Comment: Submitted to A&A, 8 pages, 5 figures, 1 tabl

Analysis of Sunyaev–Zel’dovich effect mass–observable relations using South Pole Telescope observations of an X-ray selected sample of low-mass galaxy clusters and groups

We use microwave observations from the South Pole Telescope (SPT) to examine the Sunyaev–Zel'dovich effect (SZE) signatures of a sample of 46 X-ray selected groups and clusters drawn from ∼6 deg^2 of the XMM–Newton Blanco Cosmology Survey. These systems extend to redshift z = 1.02 and probe the SZE signal to the lowest X-ray luminosities (≥10^(42) erg s^(−1)) yet; these sample characteristics make this analysis complementary to previous studies. We develop an analysis tool, using X-ray luminosity as a mass proxy, to extract selection-bias-corrected constraints on the SZE significance and Y500 mass relations. The former is in good agreement with an extrapolation of the relation obtained from high-mass clusters. However, the latter, at low masses, while in good agreement with the extrapolation from the high-mass SPT clusters, is in tension at 2.8σ with the Planck constraints, indicating the low-mass systems exhibit lower SZE signatures in the SPT data. We also present an analysis of potential sources of contamination. For the radio galaxy point source population, we find 18 of our systems have 843 MHz Sydney University Molonglo Sky Survey sources within 2 arcmin of the X-ray centre, and three of these are also detected at significance >4 by SPT. Of these three, two are associated with the group brightest cluster galaxies, and the third is likely an unassociated quasar candidate. We examine the impact of these point sources on our SZE scaling relation analyses and find no evidence of biases. We also examine the impact of dusty galaxies using constraints from the 220 GHz data. The stacked sample provides 2.8σ significant evidence of dusty galaxy flux, which would correspond to an average underestimate of the SPT Y_(500) signal that is (17 ± 9) per cent in this sample of low-mass systems. Finally, we explore the impact of future data from SPTpol and XMM-XXL, showing that it will lead to a factor of 4 to 5 tighter constraints on these SZE mass–observable relations

The Santa Fe Light Cone Simulation Project: II. The Prospects for Direct Detection of the WHIM with SZE Surveys

Detection of the Warm-Hot Intergalactic Medium (WHIM) using Sunyaev-Zeldovich effect (SZE) surveys is an intriguing possibility, and one that may allow observers to quantify the amount of "missing baryons" in the WHIM phase. We estimate the necessary sensitivity for detecting low density WHIM gas with the South Pole Telescope (SPT) and Planck Surveyor for a synthetic 100 square degree sky survey. This survey is generated from a very large, high dynamic range adaptive mesh refinement cosmological simulation performed with the Enzo code. We find that for a modest increase in the SPT survey sensitivity (a factor of 2-4), the WHIM gas makes a detectable contribution to the integrated sky signal. For a Planck-like satellite, similar detections are possible with a more significant increase in sensitivity (a factor of 8-10). We point out that for the WHIM gas, the kinematic SZE signal can sometimes dominate the thermal SZE where the thermal SZE decrement is maximal (150 GHz), and that using the combination of the two increases the chance of WHIM detection using SZE surveys. However, we find no evidence of unique features in the thermal SZE angular power spectrum that may aid in its detection. Interestingly, there are differences in the power spectrum of the kinematic SZE, which may not allow us to detect the WHIM directly, but could be an important contaminant in cosmological analyses of the kSZE-derived velocity field. Corrections derived from numerical simulations may be necessary to account for this contamination.Comment: 9 pages, submitted to Astrophysical Journa