Family House

Subject of the bachelor´s thesis is single-family, serial familial house in Brno-Chrlice. The building is designed in flat terrain. The main entrance is oriented to the southeast. Consists of one basement and two storey (floor). The house is bulit from building systém VELOX. Roof is flat with green, roof of outbuilding is saddle

Family House

Předmětem bakalářské práce je jednogenerační řadový rodinný dům v Brně-Chrlicích. Objekt je navržen v rovinatém terénu. Hlavní vstup je orientován na jihovýchod. Skládá se z jednoho podzemního a dvou nadzemních podlaží. Budova je postavena ze stavebního systému VELOX. Střecha je plochá se zelení, střecha přístavku je sedlová.Subject of the bachelor´s thesis is single-family, serial familial house in Brno-Chrlice. The building is designed in flat terrain. The main entrance is oriented to the southeast. Consists of one basement and two storey (floor). The house is bulit from building systém VELOX. Roof is flat with green, roof of outbuilding is saddle.

Physico-chemical characterization of bilirubin-10-sulfonate and comparison of its acid–base behavior with unconjugated bilirubin

Abstract Unconjugated bilirubin (UCB) is the end-product of heme catabolism in the intravascular compartment. Although beneficial for human health when mildly elevated in the body, when present at greater than a critical threshold concentration, UCB exerts toxic effects that are related to its physico-chemical properties, particularly affecting the central nervous system. The aim of the present study was to characterize bilirubin-10-sulfonate (ranarubin), a naturally occurring bile pigment, including determination of its mixed acidity constants (pK a *). Thanks to the presence of the sulfonic acid moiety, this compound is more polar compared to UCB, which might theoretically solve the problem with an accurate determination of the UCB pK a * values of its propionic acid carboxylic groups. Bilirubin-10-sulfonate was synthesized by modification of a previously described procedure; and its properties were studied by mass spectrometry (MS), nuclear magnetic resonance (NMR), infrared (IR), and circular dichroism (CD) spectroscopy. Determination of pK a * values of bilirubin-10-sulfonate and UCB was performed by capillary electrophoresis with low pigment concentrations in polar buffers. The identity of the synthesized bilirubin-10-sulfonate was confirmed by MS, and the pigment was further characterized by NMR, IR, and CD spectroscopy. The pK a values of carboxylic acid moieties of bilirubin-10-sulfonate were determined to be 5.02, whereas those of UCB were determined to be 9.01. The physico-chemical properties of bilirubin-10-sulfonate were partially characterized with low pK a * values compared to those of UCB, indicating that bilirubin-10-sulfonate cannot be used as a surrogate pigment for UCB chemical studies. In addition, using a different methodological approach, the pK a * values of UCB were found to be in a mildly alkaline region, confirming the conclusions of a recent critical re-evaluation of this specific issue