Examining the Nuance in Public Opinion of Pro-LGB Policies in a Red State.

The red-blue state and urban–rural narratives— which depict that people living in red states and rural areas oppose pro-LGB policies—are popular frames for describing variation in public opinion of LGB policies by geographic re- gion. In a test case of a red state, we examine public opinion of pro-LGB policies to assess the accuracy of the red–blue and urban–rural narratives. Using data from a survey of Nebraskans (n = 1608), we found that public opinion was more nuanced than the red state narrative allows but that urban and rural respondents reported significantly different opinions of pro- LGB policies. Rural people, however, were not unsupportive of all pro-LGB policies. Among all Nebraskans, support was higher for policies to protect LGB people from housing and job discrimination while support was lower for marriage and adop- tion rights. We discuss what these findings mean for public policy, urban and rural LGB individuals, and future public opinion studies of LGB issues

Backlash or a Positive Response? Public Opinion of LGB Issues after Obergefell v. Hodges.

Following Obergefell v. Hodges, same-sex marriage remains controversial and anti-LGBT state legislation has been passed, which raises questions about whether the Supreme Court’s ruling may have created a backlash. We use data from two waves of a general population survey of Nebraskans conducted before and after the decision to answer three questions. First, we test three theories of how the Court decision influenced public opinion. We find that support for same-sex marriage was significantly higher following the ruling, suggesting that there was not a backlash to it. Second, we assess whether people perceive that the court accurately reflects the public’s opinion. We find that people who favor same-sex marriage are more likely to think that the ruling refects public opinion very well; those who oppose same-sex marriage are more likely to think that the ruling does not at all reflect public opinion. Third, we examine the association between discussing gay rights and support for same-sex marriage, finding that those who talk about LGB issues very often are more likely to favor same-sex marriage. We discuss the implications of these findings in relation to two of the themes of this special issue: the influence of marriage equality on Americans’ understandings of marriage and the impact of marriage equality on future LGBT activism

Logics of Freedom: Debating Religious Freedom Laws and Gay and Lesbian Rights

In the wake of marriage equality for same-sex couples, many states have introduced and passed laws that provide religious exemptions for certain services and benefits for LGBTQ (lesbian, gay, bisexual, transgender, and queer) persons. The authors use data from a general population survey of Nebraskans as a mixed-methods case study to examine public opinion of religious freedom laws. Drawing on data from both closed-ended (n = 1,117) and openended (n = 838) questions, the authors show that opposition to religious freedom laws is quite high, as 64 percent of respondents report that they oppose laws that would allow business owners to deny services to gay men and lesbians. The authors outline how both sides rely on frameworks that are foundational to the American experience: the protection of rights and the capitalist economy. The authors argue that these appeals to broad American values underscore why these bills will continue to be introduced and seen as controversial despite low levels of support

Drawing on LGB Identity to Encourage Participation and Disclosure of Sexual Orientation in Surveys

This paper reports an experiment that tested how three survey cover designs—images of traditional families and individuals displaying themselves in typical gender ways; images of lesbian, gay, and bisexual (LGB) and heterosexual individuals and families; and no cover images—affected LGB people’s participation and disclosure of LGB identity and non-LGB people’s participation. Analyses showed the LGB-inclusive cover led to significantly more LGB respondents than the other designs, without significantly affecting the demographic, political, and religious makeup of the completed sample. We discuss what these findings mean for addressing two challenges: getting LGB people to respond to surveys and to disclose their LGB identity

Effects of Smiley Face Scales on Visual Processing of Satisfaction Questions in Web Surveys

Web surveys permit researchers to use graphic or symbolic elements alongside the text of response options to help respondents process the categories. Smiley faces are one example used to communicate positive and negative domains. How respondents visually process these smiley faces, including whether they detract from the question’s text, is understudied. We report the results of two eye-tracking experiments in which satisfaction questions were asked with and without smiley faces. Respondents to the questions with smiley faces spent less time reading the question stem and response option text than respondents to the questions without smiley faces, but the response distributions did not differ by version. We also find support that lower literacy respondents rely more on the smiley faces than higher literacy respondents

Effects of Smiley Face Scales on Visual Processing of Satisfaction Questions in Web Surveys

Web surveys permit researchers to use graphic or symbolic elements alongside the text of response options to help respondents process the categories. Smiley faces are one example used to communicate positive and negative domains. How respondents visually process these smiley faces, including whether they detract from the question’s text, is understudied. We report the results of two eye-tracking experiments in which satisfaction questions were asked with and without smiley faces. Respondents to the questions with smiley faces spent less time reading the question stem and response option text than respondents to the questions without smiley faces, but the response distributions did not differ by version. We also find support that lower literacy respondents rely more on the smiley faces than higher literacy respondents

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

INTRODUCTION Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic. RATIONALE We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs). RESULTS Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants. CONCLUSION Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

Tailoring General Population Surveys to Address Participation and Measurement Challenges of Surveying Lesbian, Gay, and Bisexual People

Being rare and stigmatized, lesbian, gay, and bisexual (LGB) people are hard-to-survey. Gaining their participation, reducing concealment of LGB identity, and accurately measuring their marital status are challenging. In this dissertation, I examine the effects that LGB-inclusive tailoring—inclusive cover image design and “same-sex” and “opposite-sex” marital status categories—has on addressing these challenges; particularly, the effect on who responds to a survey and the answers that they provide, among LGB and non-LGB people. The experiments were embedded in the 2013 Nebraska Annual Social Indicators Survey (NASIS), a general population mail survey of Nebraskans (n=1,608). I test how the LGB-inclusive cover design and marital status categories influenced the percent of LGB respondents; the percent of respondents who are in same-sex relationships; unit and item nonresponse; the demographic, political, and religious composition of respondents; reports to attitudinal questions about LGB issues; and how non-LGB respondents report their marital status. In the final part of this dissertation, I examine whether the red-blue state and urban-rural narratives reflect Nebraskans’ opinions about LGB issues. Analyses showed that the inclusive cover design increased the percent of LGB respondents without a significant backlash from others in the population and little effect on answers to LGB issue questions. The LGB-inclusive marital status categories, however, did not address the challenges of measuring same-sex couple identity. Instead, the inclusive wording led to higher item nonresponse and to more heterosexual respondents misreporting their marital status. Additionally, I observed that Nebraska does not fit a red state narrative, with equal favorability and opposition to same-sex marriage and majority support for other LGB rights; although, I found that rural respondents reported significantly more conservative opinions than urban respondents, consistent with that frame. Overall, this dissertation suggests that inclusive cover designs might be useful for encouraging hard-to-survey populations’ participation, that more research is necessary to accurately measure marital status, and that Nebraskans’ opinions about LGB issues are more complex than people often assume. Adviser: Jolene D. Smyt