15 research outputs found
Future Imperfect: Googling for Principles in Online Behavioral Advertising
In a remarkably short time, Google, Inc. has grown from two people working in a rented garage to a pervasive Internet force. Much of Google\u27s unprecedented success stems from online advertising sales which employ behavioral advertising techniques-techniques that track consumer behavior--thereby increasing relevance and decreasing the cost of reaching a targeted audience. In the same span that saw Google\u27s inception and explosive online dominance, the Federal Trade Commission has struggled to define not only the privacy issues involved in online behavioral advertising, but also the practice of behavioral advertising itself. Freed from the restraints of comprehensive federal laws and restrictive federal regulations, Google and its ilk have taken innovative liberties with the collection and use of consumer information. While the Federal Trade Commission ponders the subtleties of online behavioral advertising, mountains of data about consumers are being gathered and manipulated like never before, scarcely subject to legislative or regulatory privacy protections. This Note details the meteoric rise of Google before a backdrop of permissive selfregulation and argues for the establishment of baseline consumer privacy protections for online behavioral advertising
Future Imperfect: Googling for Principles in Online Behavioral Advertising
In a remarkably short time, Google, Inc. has grown from two people working in a rented garage to a pervasive Internet force. Much of Google\u27s unprecedented success stems from online advertising sales which employ behavioral advertising techniques-techniques that track consumer behavior--thereby increasing relevance and decreasing the cost of reaching a targeted audience. In the same span that saw Google\u27s inception and explosive online dominance, the Federal Trade Commission has struggled to define not only the privacy issues involved in online behavioral advertising, but also the practice of behavioral advertising itself. Freed from the restraints of comprehensive federal laws and restrictive federal regulations, Google and its ilk have taken innovative liberties with the collection and use of consumer information. While the Federal Trade Commission ponders the subtleties of online behavioral advertising, mountains of data about consumers are being gathered and manipulated like never before, scarcely subject to legislative or regulatory privacy protections. This Note details the meteoric rise of Google before a backdrop of permissive selfregulation and argues for the establishment of baseline consumer privacy protections for online behavioral advertising
Assessing the Human Resources Factors as the Determinants of Retaining Talents Across Organizations Using Multi-Level Model
Identification and Characterization of Proteins Involved in Nuclear Organization Using <em>Drosophila</em> GFP Protein Trap Lines
<div><h3>Background</h3><p>Strains from a collection of <em>Drosophila</em> GFP protein trap lines express GFP in the normal tissues where the endogenous protein is present. This collection can be used to screen for proteins distributed in the nucleus in a non-uniform pattern.</p> <h3>Methodology/Principal Findings</h3><p>We analyzed four lines that show peripheral or punctate nuclear staining. One of these lines affects an uncharacterized gene named <em>CG11138</em>. The CG11138 protein shows a punctate distribution in the nuclear periphery similar to that of <em>Drosophila</em> insulator proteins but does not co-localize with known insulators. Interestingly, mutations in Lamin proteins result in alterations in CG11138 localization, suggesting that this protein may be a novel component of the nuclear lamina. A second line affects the <em>Decondensation factor 31</em> (<em>Df31</em>) gene, which encodes a protein with a unique nuclear distribution that appears to segment the nucleus into four different compartments. The X-chromosome of males is confined to one of these compartments. We also find that <em>Drosophila</em> Nucleoplasmin (dNlp) is present in regions of active transcription. Heat shock leads to loss of dNlp from previously transcribed regions of polytene chromosome without redistribution to the heat shock genes. Analysis of Stonewall (Stwl), a protein previously found to be necessary for the maintenance of germline stem cells, shows that Stwl is present in a punctate pattern in the nucleus that partially overlaps with that of known insulator proteins. Finally we show that Stwl, dNlp, and Df31 form part of a highly interactive network. The properties of other components of this network may help understand the role of these proteins in nuclear biology.</p> <h3>Conclusions/Significance</h3><p>These results establish screening of GFP protein trap alleles as a strategy to identify factors with novel cellular functions. Information gained from the analysis of CG11138 Stwl, dNlp, and Df31 sets the stage for future studies of these proteins.</p> </div
Distribution of dNLP in various cell types.
<p>(A–C) dNLP-GFP flourescence in diploid cells from third instar larvae imaginal tissue from a dNLP protein-trap allele; DAPI (A), dNLP-GFP (B) and merged (C). Panels (D–G) depict dNLP localization in both somatic and germline cell nuclei including the oocyte nucleus (arrowhead in F and G); (D) DAPI, (E) α- Lamin Dm0, (F) α-dNLP and (G) merged. Panels H and I show dNLP-GFP fluorescence in an egg from a dNLP protein-trap allele (H) as compared to a wild type egg (I) indicating that dNLP-GFP is being dumped into the developing egg of the protein-trap allele. Panels J-N show Kc cells labeled with DAPI- blue, α-dNLP- red and α-H3S10ph-green in interphase (J), prophase (K), metaphase (L), anaphase (M) and telophase (N); the results suggest that dNLP is not associated with condensed chromosomes during metaphase. Panels (O–V). (O–R) show polytene chromosomes from wild type third instar larvae prior to heat shock while (S–V) show chromosomes from larvae subjected to a 20 min heat shock at 37°C. dNLP is broadly present in interbands and frequently colocalizes with RNA Pol II phosphorylated at Ser5 on the non-heats hocked polytene chromosomes. dNLP seems to become more diffuse and dissociate from the DNA following heat shock. The distribution of dNLP is particularly weak at the heat shock puffs, which are the only sites of transcription following temperature elevation (S–V). (O and S)-DAPI, (P and T)-α-dNLP, (Q and U)-α-PolII<sup>ser5</sup> and (R and V)-merged.</p
Stwl expression in germline stem and differentiated cells.
<p>Panels (A–D) show the germarium of a single ovariole in the ovary (lower portion of panels) as well as an early stage egg chamber (upper portion of panel). In (A) nuclei are labeled with DAPI, (B) shows α-Stwl staining, (C) outlines the germline stem cells (arrowhead) using α-Vasa and (D) shows the merged image. Stwl colocalizes with CP190 in ovarian somatic cells. Panels (E–N) show Stwl and CP190 colocalization in terminal filament cells (E–H), follicle cells (I–K), and imaginal disc cells (L–N); DAPI is blue (E), α- Stwl is red (F, I and L), α-CP190 is green (G, J and M) and in the merged panels yellow regions show colocalization (H, K and N).</p
CG11138 does not significantly colocalize with insulator proteins on polytene chromosomes or in diploid cells.
<p>Panels (A–D) show labeling of polytene chromosomes with DAPI (A), α-CG11138 (B), α-Mod(mdg4)2.2 (C) as well as the merged image (D). Panels E–H show an enlarged portion of the chromosome displayed in panels A–D in the region defined by the white arrows. Panels I–L show a second region of a different chromosome labeled with antibodies to CG11138 and Mod(mdg4)2.2, further underscoring the limited amount of colocalization between these two proteins. Panels M-O show diploid cells from OR third instar imaginal tissue labeled with DAPI (M), α- CG11138 (N) and α-CP190 (O). Panel P shows the merged image indicating that CP190 and CG11138 do not overlap in diploid cells and specifically the bodies formed by each protein appear mutually exclusive. Panels Q–T show diploid cells from OR third instar imaginal tissue labeled with DAPI (Q), α- CG11138 (R) and α-dTopors (S). Panel T shows the merged image indicating that dTopors and CG11138 do not significantly overlap in diploid cells and specifically the bodies formed by each protein appear mutually exclusive with some exceptions were they seem to overlap.</p