GRB000301C with peculiar afterglow emission

The CCD magnitudes in Johnson V and Cousins R and I photometric passbands are determined for GRB 000301C afterglow starting ~ 1.5 day after the gamma-ray burst. In fact we provide the earliest optical observations for this burst. Light curves of the afterglow emissions in U, B, V, R, I, J and K' passbands are obtained by combining the present measurements with the published data. Flux decay shows a very uncommon variation relative to other well observed GRBs. Overall, there is a steepening of the optical and near-infrared flux decay caused by a geometric and sideways expanding jet. This is superimposed by a short term variability especially during early time (Delta t < 8 days). The cause of variability is not well understood, though it has occurred simultaneously with similar amplitude in all the filters. We derive the early and late time flux decay constants using jet model. The late time flux decay is the steepest amongst the GRB OTs observed so far with alpha ~ 3. Steepening in the flux decay seems to have started simultaneously around Delta t ~ 7.6 day in all passbands. The value of spectral index in the optical-near IR region is ~ -1.0. Redshift determination with z=2.0335 indicates cosmological origin of the GRB having a luminosity distance of 16.6 Gpc. Thus it becomes the second farthest amongst the GRBs with known distances. An indirect estimate of the fluence > 20 keV indicates, if isotropic,> =10^53 ergs of release of energy. The enormous amount of released energy will be reduced, if the radiation is beamed which is the case for this event. Using a jet break time of 7.6 days, we infer a jet opening angle of ~ 0.15 radian. This means the energy released is reduced by a factor of ~ 90 relative to the isotropic value.Comment: LaTeX file, 11 pages including 4 figures, uses psfig.sty, Bull. Astron. Society of India(accepted, Sept, 2000 issue

Biogenic synthesis of gold nanoparticles using Sargassum tenerrimum and its evaluation of antibacterial activity against Escherichia coli and Salmonella typhi

1291-1297An important aspect of nanotechnology is the development of a reliable and ecofriendly method for the synthesis of nanomaterials. In the present study, Sargassum tenerrimum extract was used to synthesize gold nanoparticles (AuNPs) by the reduction of AuCl4− ions to Au0. The formation and morphology of the synthesized AuNPs were investigated using \UV–visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, X-ray diffraction, and field emission scanning electron microscopy with energy dispersive X-ray analysis. The nanoparticles synthesized at 60 °C were spherical in shape while few were also hexagonal in shape with the size ranging between 10 and 40 nm. The antibacterial activity of AuNPs was tested against Escherichia coli (ATCC 25922) and Salmonella typhi (ATCC 6539). On examination under an epifluorescence microscope, it was found to cause a significant amount of deterioration to the bacterial cells

Optical and Radio observations of the bright GRB010222 afterglow: evidence for rapid synchrotron cooling?

We report photometric observations of the optical afterglow of GRB010222 in V,R and I passbands carried out at UPSO, Naini Tal between 22-27 Feb 2001. We determine CCD Johnson BV and Cousins RI photometric magnitudes for 31 stars in the field of GRB010222 and use them to calibrate our measurements as well as other published BVRI photometric magnitudes of GRB010222 afterglow. We construct the light curve in V,R,I passbands and from a broken power-law fit determine the decay indices of 0.74+/-0.05 and 1.35+/-0.04 before and after the break at 0.7 days. Using reported X-ray flux measurements at 0.35 and 9.13 days after the burst we determine X-ray to opt/IR spectral index of 0.61+/-0.02 and 0.75+/-0.02 on these two days. We also report upper limits to the radio flux obtained from the RATAN-600 telescope and the GMRT, and millimeter-wave upper limits obtained from the Plateau de Bure Millimeter interferometer. We argue that the synchrotron cooling frequency is below the optical band for most of the observing period. We also estimate an initial jet opening angle of about 2.0n^(1/8) degrees, where n is the number density of the ambient medium.Comment: 16 pages, 4 postscript figures, minor revisions according to referee's comments, millimeter upper limit added, accepted for publication in Bulletin of the Astronomical Society of Indi

Involvement of Enterobacter cloacae in the mortality of the fish, Mugil cephalus

Enterobacter cloacae, an enteric bacterium that belongs to the family Enterobacteriaceae, is widely distributed in nature. It is found in faeces of humans and animals, water, soil, plants, plant materials, insects and dairy product

Multiband optical monitoring of the blazars S5 0716+714 and BL Lacertae

We report results of multiband optical monitoring of two well known blazars, S5 0716+714 and BL Lacertae, carried out in 1996 and 2000-01 with an aim to study optical variations on time scales from minutes to hours and longer.The light curves were derived relative to comparison stars present on the CCD frames. Night to night flux variations of >0.1 mag were observed in S5 0716+714 during a campaign of ~2 weeks in 1996.A good correlation between the lightcurves in different optical bands was found for both inter-night and intra-night observations. Two prominent events of intra-night optical variability were detected in S5 0716+714.Each of these rapidly varying segments of the lightcurves trace an exponential flux profile whose rate of variation is the same in both cases. Our long-term monitoring data of S5 0716+714 showed a distinct flare around JD 2451875 which can be identified in the BVRI bands.This flare coincides with the brightest phase recorded during 1994-2001 in the long-term lightcurves reported by Raiteri et al.(2003). No evidence for the bluer when brighter trend was noticed on inter-night and intra-night time scales. On the other hand, our nearly simultaneous multiband observations of BL Lacertae in October 2001 showed flux variations that were not achromatic. This blazar was found to become bluer when brighter on intra-night time scales and there is a hint of the same trend on inter-night time scales. Based on five nights of observations during a week, BL Lacertae showed a peak night-to-night variability of ~0.6 mag in B. Thus, we found that the present observations of the two blazars, reveal a contrasting behaviour in terms of the dependence of spectral hardening with increasing brightness, at least on intra-night, and possibly also on inter-night, time scales.Comment: 11 pages with 10 figures,uses mn2e.cls, Accepted for publication in MNRAS. Tables 4 and 5 in full will be available in the electronic version of the articl

Proteomic and functional analyses of the virion transmembrane proteome of cyprinid herpesvirus 3

Virion transmembrane proteins (VTPs) mediate key functions in the herpesvirus infectious cycle. Cyprinid herpesvirus 3 (CyHV-3) is the archetype of fish alloherpesviruses. The present study was devoted to CyHV-3 VTPs. Using mass spectrometry approaches, we identified 16 VTPs of the CyHV-3 FL strain. Mutagenesis experiments demonstrated that eight of these proteins are essential for viral growth in vitro (ORF32, ORF59, ORF81, ORF83, ORF99, ORF106, ORF115, and ORF131), and eight are non-essential (ORF25, ORF64, ORF65, ORF108, ORF132, ORF136, ORF148, and ORF149). Among the non-essential proteins, deletion of ORF25, ORF132, ORF136, ORF148, or ORF149 affects viral replication in vitro, and deletion of ORF25, ORF64, ORF108, ORF132, or ORF149 impacts plaque size. Lack of ORF148 or ORF25 causes attenuation in vivo to a minor or major extent, respectively. The safety and efficacy of a virus lacking ORF25 were compared to those of a previously described vaccine candidate deleted for ORF56 and ORF57 (Δ56-57). Using quantitative PCR, we demonstrated that the ORF25 deleted virus infects fish through skin infection and then spreads to internal organs as reported previously for the wild-type parental virus and the Δ56-57 virus. However, compared to the parental wild-type virus, the replication of the ORF25 deleted virus was reduced in intensity and duration to levels similar to those observed for the Δ56-57 virus. Vaccination of fish with a virus lacking ORF25 was safe but had low efficacy at the doses tested. This characterization of the virion transmembrane proteome of CyHV-3 provides a firm basis for further research on alloherpesvirus VTPs. IMPORTANCE Virion transmembrane proteins play key roles in the biology of herpesviruses. Cyprinid herpesvirus 3 (CyHV-3) is the archetype of fish alloherpesviruses and the causative agent of major economic losses in common and koi carp worldwide. In this study of the virion transmembrane proteome of CyHV-3, the major findings were: (i) the FL strain encodes 16 virion transmembrane proteins; (ii) eight of these proteins are essential for viral growth in vitro; (iii) seven of the non-essential proteins affect viral growth in vitro, and two affect virulence in vivo; and (iv) a mutant lacking ORF25 is highly attenuated but induces moderate immune protection. This study represents a major breakthrough in understanding the biology of CyHV-3 and will contribute to the development of prophylactic methods. It also provides a firm basis for the further research on alloherpesvirus virion transmembrane proteins

Idylls of socialism : the Sarajevo Documentary School and the problem of the Bosnian sub-proletariat

This historical overview of the Sarajevo Documentary School considers the films, in the light of their recent re-emergence, as indicative of both the legacy of socialist realism (even in the context of Yugoslav media) and attempted social engineering in the Bosnia of the 1960s and 1970s. The argument is made that the documentaries, despite their questionable aesthetic status (in respect of cinma-vrit and ethnography) and problematic ideological strategies and attempted interventions, document a history and offer insights that counter the prevailing revisionist trends in the presentation of Eastern and Central European history