8 research outputs found
<span style="font-size:14.0pt;line-height: 115%;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#252525;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">Influence of <span style="font-size:14.0pt;line-height: 115%;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#0F0F0F;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">nutritional <span style="font-size:14.0pt;line-height: 115%;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#3B3B3B;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">supp<span style="font-size:14.0pt;line-height:115%; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#0F0F0F;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">lements <span style="font-size:14.0pt;line-height:115%; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#252525;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">on keratinolysis by <i>Am</i><i><span style="font-size: 14.0pt;line-height:115%;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";color:#4C4C4C;mso-ansi-language:EN-IN;mso-fareast-language: EN-IN;mso-bidi-language:HI" lang="EN-IN">yc<span style="font-size:14.0pt; line-height:115%;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#252525;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">olatopsis keratinophila</span></span></i></span></span></span></span></span>
870-874Keratinolytic
potential of A. keratinophila (DSM 44409T) , a newly
described Amycolatopsis sp. isolated
from cultivated soil in Kuwait, was demonstrated using keratinazure as the sole
source of carbon and nitrogen as estimated by gel diffusion assay . Effects of
12 various nutritional supplements on the keratinolytic and azocollytic
activities were determined .NH4H2PO4 and KNO3
in the medium supported a significantly higher keratinolytic activity than
other supplements. However, azocollytic activities in all the supplemented
media and the control were same. Best combination of carbon and nitrogen
supplements (galactose and NH4H2PO4 respectively)
used to evaluate the dynamics of growth and enzymes (keratinase and protease)
activities of the isolate revealed a luxuriant growth with optimal
keratinolytic activity occurring during the log phase. Other parameters of the
fermentation medium, including pH, biomass accumulation, total protein
and free amino acid concentrations were also studied