2,542 research outputs found
āI had to pop a wheelie and pay extra attention in order not to fall:ā embodied experiences of two wheelchair tennis athletes transgressing ableist and gendered norms in disability sport and university spaces
When bodies move in certain contexts, it can mean very different things for different people. In our society, some bodies are more valued than others, and detrimentally, this can mean that certain types of bodies are ostracised and segregated to the outskirts of production economies and society. Dis/ability sport spaces, able-bodied sports spaces and able-bodied university spaces have been an under-researched area when considering how the body moves throughout these spaces for elite wheelchair athletes taking part in university courses. To learn more, this paper drew on feminist poststructuralism and new materialist perspectives and shared an insight into how two athletes with dis/abilities transgressed abled and gendered norms in different spaces and how they positioned themselves as athletic bodies and disabled bodies in these spaces. Employing a post-critical ethnographic design, we found that dependent on the space a dis/abled body is in constant flux as to when it feels marginalised and different (typically able-bodied spaces) and when it feels included, valued, and strong (typically dis/abled spaces). Significantly, the materiality of the institutional structures of universities, founded upon historic aesthetics of beauty dictated the physical spaces the athletes entered and created spaces of exclusion based on capitalist and ableist ideologies
Metabolic effects of overnight continuous infusion of unacylated ghrelin in humans
Objective: To clarify the metabolic effects of an overnight i.v. infusion of unacylated ghrelin (UAG) in humans. UAG exerts relevant metabolic actions, likely mediated by a still unknown ghrelin receptor subtype, including effects on Ī²-cell viability and function, insulin secretion and sensitivity, and glucose and lipid metabolism. Design: We studied the effects of a 16-h infusion (from 2100 to 1300 h) of UAG (1.0 Ī¼g/kg per h) or saline in eight normal subjects (age (meanĀ±S.E.M.), 29.6Ā±2.4 years; body mass index (BMI), 22.4Ā±1.7 kg/m2), who were served, at 2100 and 0800 h respectively, with isocaloric balanced dinner and breakfast. Glucose, insulin, and free fatty acid (FFA) levels were measured every 20 min. Results: In comparison with saline, UAG induced significant (P<0.05) changes in glucose, insulin, and FFA profiles. UAG infusion decreased glucose area under the curve (AUC) values by 10% (UAG0-960 min: 79.0Ā±1.7Ć10 3 mg/dl per min vs saline0-960 min: 87.5Ā±3. 8Ć103 mg/dl per min) and the AUC at night by 14% (UAG 180-660 min: 28.4Ā±0.5Ć103 mg/dl per min vs saline180-660 min: 33.2Ā±1.1Ć103 mg/dl per min). The overall insulin AUC was not significantly modified by UAG infusion; however, insulin AUC observed after meals was significantly increased under the exposure to UAG with respect to saline at either dinner or breakfast. The FFA AUC values were decreased by 52% under the exposure to UAG in comparison with saline (UAG0-960 min: 0.3Ā±0.02Ć103 mEq/l per min vs saline0-960 min: 0.6Ā±0.05Ć103 mEq/l per min). Conclusions: Exposure to the i.v. administration of UAG improves glucose metabolism and inhibits lipolysis in healthy volunteers. Thus, in contrast to the diabetogenic action of AG, UAG displays hypoglycemic properties
A Quasi-Classical Model of Intermediate Velocity Particle Production in Asymmetric Heavy Ion Reactions
The particle emission at intermediate velocities in mass asymmetric reactions
is studied within the framework of classical molecular dynamics. Two reactions
in the Fermi energy domain were modelized, Ni+C and Ni+Au at 34.5
MeV/nucleon. The availability of microscopic correlations at all times allowed
a detailed study of the fragment formation process. Special attention was paid
to the physical origin of fragments and emission timescales, which allowed us
to disentangle the different processes involved in the mid-rapidity particle
production. Consequently, a clear distinction between a prompt pre- equilibrium
emission and a delayed aligned asymmetric breakup of the heavier partner of the
reaction was achieved.Comment: 8 pages, 7 figures. Final version: figures were redesigned, and a new
section discussing the role of Coulomb in IMF production was include
Identification of pannexin 1-regulated genes, interactome, and pathways in rhabdomyosarcoma and its tumor inhibitory interaction with AHNAK
Rhabdomyosarcoma (RMS), the most common soft tissue sarcoma in children, is an aggressive cancer with a poor prognosis. Despite current management, the 5-year survival rate for patients with metastatic RMS is ā¼30%; underscoring the need to develop better treatment strategies. We have recently reported that pannexin 1 (PANX1) levels are downregulated in RMS and that restoring its expression inhibits RMS progression. Here, we have surveyed and characterized the molecular changes induced by PANX1 re-expression in RMS. We cataloged transcriptomic changes in this context by RNA sequencing. At the protein level, we unveiled PANX1 interactors using BioID, complemented by co-immunoprecipitation coupled to high-performance liquid chromatography/electrospray ionization tandem mass spectrometry performed in PANX1-enriched fractions. Using these data, we generated searchable public databases for the PANX1 interactome and changes to the RMS transcriptome occurring when PANX1 expression is restored. STRING network analyses revealed a PANX1 interactome involving plasma membrane and cytoskeleton-associated proteins including the previously undescribed interactor AHNAK. Indeed, AHNAK knockdown abrogated the PANX1-mediated reduction in RMS cell viability and migration. Using these unbiased approaches, we bring insight to the mechanisms by which PANX1 inhibits RMS progression, identifying the cell migration protein AHNAK as a key modifier of PANX1-mediated changes in RMS malignant properties
Neutron to proton ratios of quasiprojectile and midrapidity emission in the Zn + Zn reaction at 45 MeV/nucleon
Simultaneous measurement of both neutrons and charged particles emitted in
the reaction Zn + Zn at 45 MeV/nucleon allows comparison of the
neutron to proton ratio at midrapidity with that at projectile rapidity. The
evolution of N/Z in both rapidity regimes with increasing centrality is
examined. For the completely re-constructed midrapidity material one finds that
the neutron-to-proton ratio is above that of the overall Zn + Zn
system. In contrast, the re-constructed ratio for the quasiprojectile is below
that of the overall system. This difference provides the most complete evidence
to date of neutron enrichment of midrapidity nuclear matter at the expense of
the quasiprojectile
Exploratory Investigation on the Occurrence, Spatial Distribution, and Risk Factors of Selected Zoonotic Enteropathogens in Davao City Backyard Farms
The swine industry is the second largest economic contributor to Philippine agriculture and is dominated by backyard farms, which are plagued by outdated management practices and poor animal health support that promote the spread of pathogens. Zoonotic enteropathogens pose a public health threat, especially to backyard farmers who have daily close contact with the infected animals and their waste. Hence, there is a need to survey such pathogens. This exploratory study generated baseline information on enteropathogen occurrence in backyard farms of Davao City, Philippines; the spatial distribution of affected farms; and the risk factors for enteropathogen occurrence. Protozoans such as Blastocystis sp., Balantidium coli, Entamoeba sp., Iodamoeba sp., Giardia sp., and coccidia, while helminths such as hookworm and strongylids were identified by direct wet smear. Rotavirus A was detected by reverse transcription-nested polymerase chain reaction. Almost 73% of the farms harbored enteropathogens with mostly asymptomatic infections, and weaners and growers are major carriers. Geospatial analysis identified Barangay Bato in Toril District as a hotspot for the pathogens. Probit regression analysis revealed that use of treatments increased the likelihood of pathogen occurrence by 24%, possibly due to misapplication of medications such as anthelmintics. On the other hand, there was 40% reduced likelihood for farms that use traditional feeds, which can promote gut immunity. Therefore, high-fiber diet can be explored for broad-spectrum protection against enteropathogens. Promoting awareness on the benefits of traditional feeds and education on the proper use of medication are also recommended, especially for vulnerable farms in hotspot areas
Mixed methods, materialism and the micropolitics of the research-assemblage
We assess the potential for mixing social research methods, based upon a materialist and micropolitical analysis of the research-assemblage and of what individual research techniques and methods do in practice. Applying a DeleuzoGuattarian toolkit of assemblages, affects and capacities, we document what happens when research methods and techniques interact with the events they wish to study. Micropolitically, many of these techniques and methods have unintended effects of specifying and aggregating events, with the consequently that the knowledge produced by social inquiry is invested with these specifications and aggregations. We argue that rather than abandoning these social research tools, we may use the micropolitical analysis to assess precisely how each method affects knowledge production, and engineer the research designs we use accordingly. This forms the justification for mixing methods that are highly aggregative or specifying with those that are less so, effectively rehabilitating methods that have often been rejected by social researchers, including surveys and experiments
Functional characterization of a melon alcohol acyl-transferase gene family involved in the biosynthesis of ester volatiles. Identification of the crucial role of a threonine residue for enzyme activity
Volatile esters, a major class of compounds contributing to the aroma of many fruit, are synthesized by
alcohol acyl-transferases (AAT). We demonstrate here that, in Charentais melon (Cucumis melo var.
cantalupensis), AAT are encoded by a gene family of at least four members with amino acid identity ranging
from 84% (Cm-AAT1/Cm-AAT2) and 58% (Cm-AAT1/Cm-AAT3) to only 22% (Cm-AAT1/Cm-AAT4).
All encoded proteins, except Cm-AAT2, were enzymatically active upon expression in yeast and show
differential substrate preferences. Cm-AAT1 protein produces a wide range of short and long-chain acyl
esters but has strong preference for the formation of E-2-hexenyl acetate and hexyl hexanoate. Cm-AAT3
also accepts a wide range of substrates but with very strong preference for producing benzyl acetate.
Cm-AAT4 is almost exclusively devoted to the formation of acetates, with strong preference for cinnamoyl
acetate. Site directed mutagenesis demonstrated that the failure of Cm-AAT2 to produce volatile esters is
related to the presence of a 268-alanine residue instead of threonine as in all active AAT proteins. Mutating
268-A into 268-T of Cm-AAT2 restored enzyme activity, while mutating 268-T into 268-A abolished
activity of Cm-AAT1. Activities of all three proteins measured with the prefered substrates sharply increase
during fruit ripening. The expression of all Cm-AAT genes is up-regulated during ripening and inhibited in
antisense ACC oxidase melons and in fruit treated with the ethylene antagonist 1-methylcyclopropene
(1-MCP), indicating a positive regulation by ethylene. The data presented in this work suggest that the
multiplicity of AAT genes accounts for the great diversity of esters formed in melon
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