Implications of survey effort on estimating demographic parameters of a long-lived marine top predator

Effective management of wildlife populations rely on knowledge of their abundance, survival, and reproductive rates. Maintaining long‐term studies capable of estimating demographic parameters for long‐lived, slow‐reproducing species is challenging. Insights into the effects of research intensity on the statistical power to estimate demographic parameters are limited. Here, we investigate implications of survey effort on estimating abundance, home range sizes, and reproductive output of Indo‐Pacific bottlenose dolphins (Tursiops aduncus), using a 3‐year subsample of a long‐term, capture–recapture study off Bunbury, Western Australia. Photo‐identification on individual dolphins was collected following Pollock's Robust Design, where seasons were defined as “primary periods”, each consisting of multiple “secondary periods.” The full dataset consisted of 12 primary periods and 72 secondary periods, resulting in the study area being surveyed 24 times/year. We simulated reduced survey effort by randomly removing one, two, or three secondary periods per primary period. Capture–recapture models were used to assess the effect of survey intensity on the power to detect trends in population abundance, while individual dolphin sighting histories were used to assess the ability to conduct home range analyses. We used sighting records of adult females and their calving histories to assess survey effort on quantifying reproductive output. A 50% reduction in survey effort resulted in (a) up to a 36% decline in population abundance at the time of detection; (b) a reduced ability to estimate home range sizes, by increasing the time for individuals to be sighted on ≥30 occasions (an often‐used metric for home range analyses) from 7.74 to 14.32 years; and (c) 33%, 24%, and 33% of annual calving events across three years going undocumented, respectively. Results clearly illustrate the importance of survey effort on the ability to assess demographic parameters with clear implications for population viability analyses, population forecasting, and conservation efforts to manage human–wildlife interactions

Vessel noise levels drive behavioural responses of humpback whales with implications for whale-watching

Disturbance from whale-watching can cause significant behavioural changes with fitness consequences for targeted whale populations. However, the sensory stimuli triggering these responses are unknown, preventing effective mitigation. Here, we test the hypothesis that vessel noise level is a driver of disturbance, using humpback whales (Megaptera novaeangliae) as a model species. We conducted controlled exposure experiments (n = 42) on resting mother-calf pairs on a resting ground off Australia, by simulating whale-watch scenarios with a research vessel (range 100 m, speed 1.5 knts) playing back vessel noise at control/low (124/148 dB), medium (160 dB) or high (172 dB) low frequency-weighted source levels (re 1 μPa RMS@1 m). Compared to control/low treatments, during high noise playbacks the mother’s proportion of time resting decreased by 30%, respiration rate doubled and swim speed increased by 37%. We therefore conclude that vessel noise is an adequate driver of behavioural disturbance in whales and that regulations to mitigate the impact of whale-watching should include noise emission standards

Whale-watch vessel noise levels with applications to whale-watching guidelines and conservation

The number and size of whale-watching and swim-with-cetacean vessels are increasing worldwide, but the noise impact on targeted species depends on vessel source characteristics, which remain largely unquantified. Here, we report the acoustic characteristics from 13 whale-watching vessels from Australia and Canary Islands. Acoustic recorders were deployed to measure the frequency-weighted sound levels (for low [LF], mid [MF] and high frequency [HF] cetacean hearing types) of motor sailing, catamarans, and motor vessels operating at 4–8 kn representing the slow speed of whale-watch scenarios. The highest estimated source levels (SLs) were recorded from large catamarans with inboard engines (LF = 160 ± 3, MF = 148 ± 2, HF = 146 ± 2 dB re 1 µPa m). The lowest SLs were from smaller motor vessels and particularly by a hybrid vessel powered by electrical outboard engines (LF = 140 ± 3, MF = 136 ± 2, HF = 134 ± 2 dB re 1 µPa m). We demonstrate that at the same speed and distance, different vessels may produce very different received levels to the animals. To reduce disturbance to cetaceans we recommend tourism vessels meet a broadband (0.2–10 kHz) SL limit of <150 dB re 1 µPa (RMS) when within 500 m of cetaceans

Food-provisioning negatively affects calf survival and female reproductive success in bottlenose dolphins

Food-provisioning of wildlife can facilitate reliable up-close encounters desirable by tourists and, consequently, tour operators. Food-provisioning can alter the natural behavior of an animal, encouraging adverse behavior (e.g. begging for food handouts), and affect the reproductive success and the viability of a population. Studies linking food-provisioning to reproductive success are limited due to the lack of long-term datasets available, especially for long-lived species such as marine mammals. In Bunbury, Western Australia, a state-licensed food-provisioning program offers fish handouts to a limited number of free-ranging bottlenose dolphins (Tursiops aduncus). Coupled with long-term historical data, this small (<200 individuals), resident dolphin population has been extensively studied for over ten years, offering an opportunity to examine the effect of food-provisioning on the reproductive success of females (ntotal = 63; nprovisioned females = 8). Female reproductive success was estimated as the number of weaned calves produced per reproductive years and calf survival at year one and three years old was investigated. The mean reproductive success of provisioned and non-provisioned females was compared using Bayes factor. We also used generalized linear models (GLMs) to examine female reproductive success in relation to the occurrence of food-provisioning, begging behavior and location (within the study area). Furthermore, we examined the influence of these variables and birth order and climatic fluctuations (e.g. El Niño Southern Oscillation) on calf survival. Bayes factor analyses (Bayes factor = 6.12) and results from the best fitting GLMs showed that female reproductive success and calf survival were negatively influenced by food-provisioning. The negative effects of food-provisioning, although only affecting a small proportion of the adult females’ population (13.2%), are of concern, especially given previous work showing that this population is declining

Seasonal feeding on giant cuttlefish (Sepia apama) by Indo-Pacific bottlenose dolphins (Tursiops aduncus) in south-western Australia

We report on observations of Indo-Pacific bottlenose dolphins (Tursiops aduncus) feeding on giant cuttlefish (Sepia apama) from March 2007 to April 2013 in the temperate waters off Bunbury, south-western Australia. Seventeen feeding events were observed during the cooler months between July and September in relatively shallow coastal waters, with 12 dolphins identified as adult females. We observed behavioural sequences of complex prey-handling of cuttlefish where dolphins’ used multiple steps to remove the cuttlefish head, ink and cuttlebone before consuming the flesh of the cuttlefish mantle. Our study provides valuable information to the limited knowledge on the complex prey-handling by T. aduncus on cuttlefish in Australia, and is complementary to other known specialised foraging behaviours of bottlenose dolphins. This study also details a different behavioural sequence of cuttlefish prey-handling to that of the bottlenose dolphins in the Sado estuary, Portugal, where only the head is consumed, and to the Spencer Gulf, Australia, in that the dolphins in Bunbury carry the cuttlefish mantle over their rostrum before removing the cuttlebone. Information on S. apama in Bunbury is scarce, therefore studies on abundance, distribution and egg-laying sites are recommended in order to enable informed decision making and to understand the importance of S. apama to the diet of T. aduncus

Silver gull harassment of humpback whales in Exmouth Gulf, Western Australia

We share observations of silver gulls (Chroicocephalus novaehollandiae) harassing humpback whales (Megaptera novaeangliae) in Exmouth Gulf, Western Australia, between August and October in 2018 and 2019. Sixteen events were recorded of gulls pecking at the skin of 16 mothers and 2 calves resting on the surface. Data were collected opportunistically from a research vessel covering 5474.2 km (565 h). Events were documented through photographs and unmanned aerial vehicle video recordings and persisted for an average minimum time of 20.73 min (range 2.50–46.85 min). At least one event of a gull consistently gouging in a pre-existing lesion was recorded on a mother. Changes in behavioural activity from the whales, which varied from slipping under the surface to travelling and performing instantaneous behavioural events, were observed in 94% (15/16) of events. These are the first known records of birds attacking humpback whales. The results are comparable to early events of kelp gull (Larus dominicanus) harassment of southern right whales (Eubalaena australis) off Península Valdés, Argentina, where events have escalated over the past 50 years to consistent attacks on the whales’ skin and blubber. Future research is required to monitor the trajectory of these interactions to inform management

Ontogenetic changes in energy expenditure and resting behaviour of humpback whale mother–calf pairs examined using unmanned aerial vehicles

Context: Baleen whale calves rapidly increase in size and improve locomotion abilities, while on their low-latitude breeding ground, allowing them to undertake a successful migration to high-latitude feeding grounds. Aims: We investigated energy expenditure and resting behaviour of humpback whale (Megaptera novaeangliae) mother–calf pairs in regard to changes in calf length on an undisturbed breeding/resting ground off Exmouth Gulf, Western Australia. Methods: Data were collected from August to October in 2018 and 2019 on lactating mothers that were predominantly resting on the surface with their calf. Focal follows on mother–calf pairs (n = 101) were conducted using an unmanned aerial vehicle to obtain detailed video of behaviours and respirations (23.7 h). Body length measurements of individual whales were calculated from aerial still frames. Key results: Results on calves ranging in length from ~4–8 m demonstrated that calf respiration rate decreased with an increase in calf length and increased with presence of activity (P < 0.001). Calf inter-breath intervals became longer in duration with an increase in calf length (P < 0.01). Calf activity level and resting behaviour remained constant, with calves logging for 53% of the time their mothers were logging. Maternal respiration rate remained low and did not differ with respect to maternal or calf length. Conclusions: Results highlighted the importance of resting grounds for energy preservation, which benefits the calves’ rapid growth before migration to polar waters. Implications: Findings from the present largely undisturbed population serve as a baseline for understanding the impacts of anthropogenic disturbance on resting behaviour and energy expenditure in humpback whale mother–calf pairs globally

Author Correction: Food-provisioning negatively affects calf survival and female reproductive success in bottlenose dolphins

Correction to: Scientific Reports https://doi.org/10.1038/s41598-019-45395-6, published online 20 June 201

Home range size of adult Indo-Pacific bottlenose dolphins (Tursiops aduncus) in a coastal and estuarine system is habitat and sex-specific

This study examined sex-specific differences in home range size of adult Indo-Pacific bottlenose dolphins off Bunbury, Western Australia. We applied a new kernel density estimation approach that accounted for physical barriers to movements. A Bayesian mixture model was developed to estimate a sex effect in home range size with latent group partitioning constrained by association data. A post hoc analysis investigated group partitioning relating to the proportion of time spent in open vs. sheltered waters. From 2007 to 2013, photographic-identification data were collected along boat-based systematic transect lines (n = 586). Analyses focused on adult dolphins of known sex (sighted ≥ 30 times; n = 22 males and 34 females). The 95% utilization distributions of males varied between 27 and 187 km2 (inline image; 94.8 ± 48.15) and for females between 20 and 133 km2 (65.6 ± 30.9). The mixture model indicated a 99% probability that males had larger home ranges than females. Dolphins mostly sighted in open waters had larger home ranges than those in sheltered waters. Home ranges of dolphins sighted in sheltered waters overlapped with areas of highest human activity. We suggest that sex differences in home ranges are driven by male mating strategies, and home range size differences between habitats may be influenced by prey availability and predation risk

Foraging preferences of an apex marine predator revealed through stomach content and stable isotope analyses

Insights into the food habits of predators are essential for maintaining healthy predator populations and the functioning of ecosystems. Stomach content and stable isotope analyses were used to investigate the foraging habits of an apex predator, the Indo-Pacific bottlenose dolphin (Tursiops aduncus) in south-western Australia. A total of 2,594 prey items from 26 families were identified from the stomachs of 10 deceased stranded dolphins. Fish otoliths from stomach contents were used to identify fish to family or species level. Ninety-three percent of identified stomach contents were perciforme fishes, however, perciformes comprised only 30% of the catch during prey sampling. Gobiidae species, small fish generally <100 mm in total length, were the most prevalent family identified in dolphin stomachs, accounting for 82% of identified prey, yet Gobiidae accounted for 12.7% of the catch during prey sampling. For stable isotope analyses, tissue samples from 14 free-ranging dolphins were analyzed for nitrogen (δ15N) and carbon (δ13C) ratios. From stable isotope analyses and boat-based dolphin photo-identification surveys (n = 339, 2007–2011), results indicated niche differentiation between coastal and inshore (bay and estuarine habitat) dolphins. Carbon signatures showed that coastal dolphins had a more pelagic diet compared to a benthic diet observed in the inshore dolphins. Whereas, nitrogen signatures of inshore dolphins showed higher nitrogen levels than coastal dolphins, likely attributed to feeding on enriched prey typical of estuarian environments. Overall, these results indicated that bottlenose dolphins in the study area were selective foragers and that their foraging is specialized by the habitats most frequently used