The effects of creatine ethyl ester supplementation combined with heavy resistance training on body composition, muscle performance, and serum and muscle creatine levels

Numerous creatine formulations have been developed primarily to maximize creatine absorption. Creatine ethyl ester is alleged to increase creatine bio-availability. This study examined how a seven-week supplementation regimen combined with resistance training affected body composition, muscle mass, muscle strength and power, serum and muscle creatine levels, and serum creatinine levels in 30 non-resistance-trained males. In a double-blind manner, participants were randomly assigned to a maltodextrose placebo (PLA), creatine monohydrate (CRT), or creatine ethyl ester (CEE) group. The supplements were orally ingested at a dose of 0.30 g/kg fat-free body mass (approximately 20 g/day) for five days followed by ingestion at 0.075 g/kg fat free mass (approximately 5 g/day) for 42 days. Results showed significantly higher serum creatine concentrations in PLA (p = 0.007) and CRT (p = 0.005) compared to CEE. Serum creatinine was greater in CEE compared to the PLA (p = 0.001) and CRT (p = 0.001) and increased at days 6, 27, and 48. Total muscle creatine content was significantly higher in CRT (p = 0.026) and CEE (p = 0.041) compared to PLA, with no differences between CRT and CEE. Significant changes over time were observed for body composition, body water, muscle strength and power variables, but no significant differences were observed between groups. In conclusion, when compared to creatine monohydrate, creatine ethyl ester was not as effective at increasing serum and muscle creatine levels or in improving body composition, muscle mass, strength, and power. Therefore, the improvements in these variables can most likely be attributed to the training protocol itself, rather than the supplementation regimen

Effects of 28 days of resistance exercise while consuming commercially available pre- and post-workout supplements, NO-Shotgun® and NO-Synthesize® on body composition, muscle strength and mass, markers of protein synthesis, and clinical safety markers in males

<p>Abstract</p> <p>Purpose</p> <p>The effects of 28 days of heavy resistance training while ingesting the pre- and post-workout supplements, NO-Shotgun^®and NO-Synthesize^®were determined on body composition, muscle strength and mass, markers of protein synthesis, and clinical safety markers.</p> <p>Methods</p> <p>Nineteen non-resistance-trained males participated in a resistance training program 4 times/week for 28 days while either ingesting 27 g/day of carbohydrate (CARB) or NO-Shotgun^®30 min pre-exercise and 27 g/day of carbohydrate or NO- Synthesize^®30 min post-exercise (NOSS). Data were analyzed with separate 2 × 2 ANOVA (p < 0.05).</p> <p>Results</p> <p>Total body mass was increased in both groups (p = 0.001), but not different between groups. Fat mass was unchanged with CARB, but NOSS decreased fat mass (p = 0.026). Both groups increased fat-free mass (p = 0.001); however, the increases were greater with NOSS (p = 0.023). NOSS underwent greater increases in upper-body (p = 0.023) and lower-body (p = 0.035) strength than CARB. Myofibrillar protein significantly increased in both groups (p = 0.041), with NOSS being greater than CARB (p = 0.049). All of the MHC isoforms were significantly increased in both groups; however, NOSS was greater than CARB for MHC 1 (p = 0.013) and MHC 2A (p = 0.046). All of the myogenic regulatory factors were significantly increased in both groups; however, NOSS was greater than CARB for Myo-D (p = 0.038) and MRF-4 (p = 0.001). For the whole blood and serum clinical chemistry markers, all variables remained within normal clinical ranges.</p> <p>Conclusions</p> <p>Heavy resistance training for 28 days, with NO-Shotgun^®and NO-Synthesize^®ingested before and after exercise, respectively, significantly improved body composition and increased muscle mass and performance without abnormally impacting any of the clinical chemistry markers.</p

Effects of Capsaicin and Evodiamine Ingestion on Energy Expenditure and Fat Oxidation at Rest and After Moderately-Intense Exercise in Young Men

Capsaicin and evodiamine are two thermogenic agents each recognized for their ability to stimulate the sympathetic nervous system and are thus found in many dietary supplements. Therefore, the purpose of this study was to observe the effects that capsaicin and evodiamine have on hemodynamics, energy expenditure, and markers of lipid oxidation while at rest and after a single bout of moderate-intensity exercise in young men. In a randomized, cross-over design, 11 men orally ingested 500 mg of capsaicin, evodiamine, or placebo while at rest after 30 minutes of resting energy expenditure assessment using open-circuit spirometry. After an additional 30 minutes of rest after supplement ingestion, resting energy expenditure was assessed again for 30 minutes. After the second resting energy expenditure assessment, treadmill exercise was performed until expending approximately 500 kilocalories (~30 minutes) at 65% peak oxygen consumption. Energy expenditure was assessed for another 30 minutes into the post-exercise period. Heart rate and blood pressure, core temperature, and venous blood samples were obtained 30 minutes before and one hour after supplement ingestion (i.e. immediately pre-exercise), and immediately after and 45 minutes post-exercise. Markers of lipid oxidation (serum glycerol, free fatty acids, serum glucose, epinephrine, and norepinephrine) were determined spectrophotometrically and with ELISA. Two-way analyses of variance (ANOVA) were performed for each dependent variable (p ≤ 0.05). Significant main effects for Time existed for hemodynamics, energy expenditure, serum catecholamines and markers of fat oxidation immediately following exercise (p \u3c 0.05). However, no significant Supplement x Time interactions were noted for any criterion variable (p \u3e 0.05), suggesting no preferential difference between supplements. Neither capsaicin nor evodiamine, at a single dose of 500 mg, are effective at inducing thermogenesis and increasing fat oxidation at rest or during exercise in young men

Effect of Pre-Exercise Nutrition on Human Skeletal Muscle UCP3 Expression

BACKGROUND: Increased UCP3 expression in skeletal muscle after exercise may be attributed to elevated free fatty acids (FFA) that are known to directly activate UCP3 expression. Pre-exercise glucose consumption has shown to blunt UCP3 expression in response to exercise. Since glucose ingestion before and during exercise is typically not a common practice, the goal of the present study was to assess the effect of a multi-macronutrient meal (drinkable shake) in the pre-exercise period towards human skeletal muscle UCP3 expression. METHODS: Using a crossover design, untrained participants performed an endurance exercise session (350 kcal at 70% of their VO2max) after two experimental conditions 1) consumption of a multi-macronutrient meal and 2) a fasting period of 8 h. Blood samples were taken at baseline, pre-exercise, post-exercise, 1h, and 4h post-exercise, while muscle biopsies were taken at the last four time points. RESULTS: A significant increase in FFA was observed in the fasting condition (p= 0.046) as well as a significant increase in UCP3 mRNA and protein expression at post-exercise (p= 0.042) and 4 h post-exercise (p= 0.036) respectively, in the multi-macronutrient meal condition. DISCUSSION: Variables showed a total opposite response to what has been reported after the consumption of pure glucose before an exercise session. Instead of observing a decrease in UCP3 expression in the non-fasting condition, we observed a significant increase in UCP3 mRNA and protein concentration in the multi-macronutrient condition. According to previous research, variables such as the protein and fat content from the multi-macronutrient meal, as well as the insulin levels, could have played key roles in altering UCP3 mRNA and protein expression in the multi-macronutrient condition; however, further research is needed to confirm this hypothesis. CONCLUSION: The expression of UCP3 mRNA and protein expression as a result of exercise might be controlled by factors other than FFA

Effect of Resistance Exercise Intensity on the mRNA Expression of PGC-1α Isoforms in Human Skeletal Muscle

Download PD

Proceedings of the Thirteenth International Society of Sports Nutrition (ISSN) Conference and Expo

Meeting Abstracts: Proceedings of the Thirteenth International Society of Sports Nutrition (ISSN) Conference and Expo Clearwater Beach, FL, USA. 9-11 June 201

The effects of creatine ethyl ester supplementation combined with resistance training on body composition, muscle mass and performance, and intramuscular creatine uptake in males.

Includes bibliographical references (p. 108-112)Creatine monohydrate has become one of the most popular ingested nutritional supplements due to its potential enhancement of athletic performance. Creatine absorption from the serum into skeletal muscle occurs through the utilization of a membrane-spanning protein, CreaT1. Numerous creatine formulations have been developed primarily to maximize creatine absorption. Creatine ethyl ester (CEE) has been chemically modified by adding an ester group and is thought to increase creatine bio-availability by by-passing the CreaT1. This study examined how a seven week supplementation regimen with CEE affected body composition, muscle mass and performance, whole body creatine retention, as well physiological and molecular adaptations, associated with creatine uptake in nonresistance-trained males following a resistance-training program. Results demonstrated that CEE did not show any additional benefit to increases in muscle strength/performance or a significant increase in total muscle creatine when compared to creatine monohydrate or placebo. CEE supplementation did show a large increase in creatinine levels throughout the study.by Mike Spillane.M.S.Ed

Effects of lower- and higher- volume resistance exercise on serum testosterone and skeletal muscle androgen receptor content in men : subsequent effects on the mRNA expression of insulin- like growth factor peptide and myostatin in skeletal muscle.

Testosterone is the primary sex steroid hormone within males. Testosterone effects are ubiquitous and are categorized as either anabolic or androgenic. The androgen receptor is a specific nuclear hormone receptor through which testosterone elicits its effects. Specifically, skeletal muscle has androgen receptors present and is responsive to testosterone. The resulting activation of testosterone–androgen receptor–DNA binding increases muscle protein synthesis and reduces muscle protein breakdown. In addition, the testosterone level can alter the expression of insulin–like growth factor and myostatin which are key regulators of muscle protein balance. Higher-intensity resistance exercise in non-resistance-trained participants is known to increase endogenous serum testosterone levels. However, using resistance-trained participants the purpose of this study was to examine whether elevations in serum testosterone occur in response to a higher-intensity of resistance-exercise, and if this increase induces elevations in skeletal muscle testosterone, 5α-dihydrotestosterone (DHT), androgen receptor mRNA and protein content as well as possible interactions between muscle IGF-1 and myostatin mRNA expression. In a randomized cross-over design, venous blood was obtained in male participants immediately before and after, 30 minutes, 1 hour, 2 hours, 3 hours, and 24 hours after a single bout of resistance exercise. Muscle samples were obtained immediately before and after, 3 hours, 24 hours after exercise. Exercise bouts consisted of an upper-lower body (higher-volume) and lower-body (lower-volume) protocol. Each exercise bout was separated by one week of rest. Statistical analyses were performed by separate 2 x 7 and 2 x 4 (Session x Test) factorial analyses of variance (ANOVA) with repeated measures. The exercise protocol employed has previously been demonstrated to significantly increase serum testosterone. However, this study did not produce such a response. Neither exercise protocol significantly increased serum total or free testosterone (p > 0.05). Also, no changes were observed for muscle testosterone, DHT, androgen receptor mRNA expression or protein content (p > 0.05). In addition, no alterations in muscle IGF-1 or myostain were observed (p > 0.05). It was concluded that higher-intensity resistance exercise of the upper-body performed immediately prior to lower-body resistance exercise does not result in a significant elevation in testosterone in resistance-trained participants.Ph.D