Darryn Willoughby

Melodie Minter

Mike Spillane

Neil Schwarz

null null

Sarah Leddy

Tracie Correa

Victoria Longoria

English

PubMed

Springer - Publisher Connector

POSTER PRESENTATION Open Access
Effects of twenty-eight days of resistance exercise
while consuming the commercially available pre-
and post-workout supplements, NO-Shotgun®
and NO-Synthesize®, on body composition,
muscle mass and performance, and clinical safety
markers in men
Mike Spillane*, Neil Schwarz, Sarah Leddy, Tracie Correa, Melodie Minter, Victoria Longoria, Darryn Willoughby,
FISSN
From International Society of Sports Nutrition: 8th Annual ISSN Conference and Expo
Las Vegas, NV, USA. 24-25 June 2011
Background
This study determined the effects of 28 days of heavyre-
sistance exercise combined with the pre- and post-work-
out nutritional supplements, NO-Shotgun® and NO-
Synthesize® onbody composition, muscle strength and
mass, markers of protein synthesis and satellite cell acti-
vation, and blood clinical safety markers.
Methods
Nineteen non-resistance-trained males were baseline
tested and then randomly assigned to participate in a
group that engaged in a resistance training program (3
X 8-10-RM) 4 times/wk for 28 days while also ingesting
54 g/day of placebo maltodextrose(PLC) or 27 g/day of
NO-Shotgun®and 27 g/day of NO-Synthesize® (NOSS).
For PLC, 27 g were ingested 30 min prior to exerciseand
27 g within 30 min following exercise. NOSS ingested
27 g of NO-Shotgun® 30 min prior to exercise and 27
g/day NO-Synthesize®within 30 min following exercise.
Immediately upon waking on non-training days, PLC
ingested 27 g of the supplement, whereas NOSS
ingested 27 g of NO-Synthesize®. On day 29, partici-
pants were subjected to follow-up testing. Data were
analyzed with separate 2 x 2 ANOVA (p < 0.05).
Results
For dietary intake, there were no significant differences
in total calories/day (p = 0.129) or in the daily amount
of protein (p = 0.216), carbohydrate (p = 0.106), and fat
(p = 0.665) between groups. For total body mass, both
groups increased with training (p = 0.01), but there was
no difference between groups (p = 0.793). However,
NOSS underwent significant improvements in fat mass
(p = 0.226) and fat-free mass (p = 0.023) compared to
PLC. Both groups significantly increased muscle
strength with training; however, for bench press (p =
0.023) and leg press (p = 0.035) NOSS was significantly
greater than PLC. Serum IGF-1 (p = 0.038) and HGF (p
= 0.001) were significantly increased with training, but
were not different between groups. Myofibrillar protein
increased in both groups with training (p = 0.041), with
NOSS being significantly greater than PLC (p = 0.050).
The levels of Type I, IIA, and IIX MHC were increased
in both groups with training; however, Type I (p =
0.013) and IIA (p = 0.05) were significantly greater in
NOSS. Muscle c-met was increased with training for
both groups (p = 0.030), but not different between
groups (p = 0.496). For total DNA, there was no differ-
ence between groups (p = 0.322) and neither group was
affected by training (p = 0.151). All of the myogenic reg-
ulatory factors were increased with training; however,
NOSS was significantly greater than PLC for Myo-D (pExercise and Biochemical Nutrition Lab, Department of Health, Human
Performance, and Recreation. Baylor University, Waco, TX 76798, USA
Spillane et al. Journal of the International Society of Sports Nutrition 2011, 8(Suppl 1):P24
http://www.jissn.com/content/8/S1/P24
© 2011 Spillane et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.
= 0.038) and MRF-4 (p = 0.001). No significant differ-
ences were located for any of the whole blood and
serum clinical chemistry markers (p > 0.05).
Conclusions
When combined with heavy resistance training for 28
days, NO-Shotgun® and NO-Synthesize® ingested
before and after exercise, respectively, significantly
improved body composition and increased muscle mass
and performance. In addition, this supplementation regi-
men didnot abnormally impact any of the clinical chem-
istry markers.
Funding
This study was supported by a research grant from VPX,
awarded to Baylor University.
Published: 7 November 2011
doi:10.1186/1550-2783-8-S1-P24
Cite this article as: Spillane et al.: Effects of twenty-eight days of
resistance exercise while consuming the commercially available pre-
and post-workout supplements, NO-Shotgun®® and NO-Synthesize®®,
on body composition, muscle mass and performance, and clinical
safety markers in men. Journal of the International Society of Sports
Nutrition 2011 8(Suppl 1):P24.
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Spillane et al. Journal of the International Society of Sports Nutrition 2011, 8(Suppl 1):P24
http://www.jissn.com/content/8/S1/P24
Page 2 of 2


Effects of twenty-eight days of resistance exercise while consuming the commercially available pre- and post-workout supplements, NO-Shotgun® and NO-Synthesize®, on body composition, muscle mass and performance, and clinical safety markers in men

Effects of twenty-eight days of resistance exercise while consuming the commercially available pre- and post-workout supplements, NO-Shotgun® and NO-Synthesize®, on body composition, muscle mass and performance, and clinical safety markers in men

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