Isolation And Characterization Of Microsatellite Loci In Paspalum Notatum Flüggé (poaceae)

Paspalum notatum is a forage grass recognized as one of the major constituents of the native grasslands in the New World. The knowledge of the genetic diversity and structure of P. notatum populations is fundamental for the conservation and germplasm management of this species. About 11 microsatellite markers were isolated from P. notatum and characterized in 25 accessions. The average number of alleles per locus was 7.9 and the PIC ranged from 0.36 to 0.89. The data demonstrated that the most of markers are suitable to detect polymorphism and to study the genetic diversity in the P. notatum species. Moreover, the transferability of these microsatellite were tested on other three congeneric species. © 2009 Springer Science+Business Media B.V.10619771980Billotte, N., Lagoda, P.J.R., Risterucci, A.M., Baurens, F.C., Microsatellite-enriched libraries: Applied methodology for the development of SSR markers in tropical crops (1999) Fruits, 54, pp. 277-288Burton, G.W., Bahia grass types (1946) Agron J, 38, pp. 273-281Burton, G.W., The method of reproduction in common bahiagrass, Paspalum notatum (1948) Agron J, 40, pp. 443-452Chase, A., The North American species of Paspalum (1929) Contr. US Natl. Herb., 28, pp. 1-310Cidade, F.W., Dall'agnol, M., Bered, F., Souza-chies, T.T., Genetic diversity of the complex Paspalum notatum Flügge (Paniceae-Panicoideae) (2008) Genet Resour Crop Evol, 55, pp. 235-246Clayton, W.D., Renvoize, S., (1986) A. Genera Graminum: Grasse of the World, , London: Royal Botanic GardensCreste, S., Tulmann Neto, A., Figueira, A., Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining (2001) Plant Mol Biol Rep, 19, pp. 299-306Daurelio, L.D., Espinoza, F., Quarín, C.L., Pession, S.C., Genetic diversity in sexual diploid and apomictic tetraploid populations of Paspalum notatum situated in sympatry or allopatry (2004) Plant Syst Evol, 244, pp. 189-199Doyle, J.J., Doyle, J.L., A rapid DNA isolation procedure for small quantities of fresh leaf tissue (1987) Phytochemistry Bull, 19, pp. 11-15Quarín, C.L., Norrmann, G.A., Urbani, M.H., Polyploidization in aposporous Paspalum species (1989) Apomixis Newsl, 2, pp. 44-46Temnykh, S., DeClerck, G., Lukashova, A., Lipovich, L., Catinhour, S., McCouch, S., Computational and experimental analysis of microsatellites in rice (Oryza sativa 1.): Frequency, length variation, transposon associations, and genetic marker potential (2001) Genome, 11, pp. 1441-1452. , doi:10.1101/gr.184001Tessier, C., This, D.P., Boursiquot, J.M., Charrier, A., Optimization of the choice of molecular markers for varietal identification Vitis vinifera (1999) L. Theor Appl Genet, 98, pp. 171-177. , doi:10.1007/s00122005105