Expanded Bed Adsorption Of Bromelain (e.c. 3.4.22.33) From Ananas Comosus Crude Extract

This work focuses on the adsorption of Bromelain in expanded bed conditions, such as the adsorption kinetics parameters. The adsorption kinetics parameters showed that after 40 minutes equilibrium was achieved and maximum adsorption capacity was 6.11 U per resin mL. However, the maximum adsorption capacity was only determined by measuring the adsorption isotherm. Only by the Langmuir model the maximum adsorption capacity, Qm, and dissociation constant, kd, values could be estimated as 9.18 U/mL and 0.591, respectively, at 25°C and 0.1 mol/L phosphate buffer pH 7.5. A column made of glass with an inner diameter of 1 cm was used for the expanded bed adsorption (EBA). The residence time was reduced 10 fold by increasing the expansion degree 2.5 times; nonetheless, the plate number (N) value was reduced only 2 fold. After adsorption, the bromelain was eluted in packed bed mode, with a downward flow. The purification factor was about 13 fold and the total protein was reduced 4 fold. EBA showed to be feasible for purification of bromelain.261149157Amersham Pharmacia Biotech. EBA Handbook: Principles and Methods. Uppsala, ISBN 91-630-5519-8 (1997)Anspach, F.B., Curbelo, D., Hartman, R., Garke, G., Deckwer, W.D., Expanded-bed chromatography in primary protein purification (1999) J. Chromatogr. A, 865, p. 129Bruce, L.J., Chase, H.A., Hydrodynamics and adsorption behaviour within an expanded bed adsorption column studied using in-bed sampling (2001) Chem. Eng. Sci, 56, p. 3149Camprubi, S., Bruguera, M., Canalias, F., Purification of recombinant histidine-tag streptolysin O using immobilized metal affinity expanded bed adsorption (IMA-EBA). International (2006) J. Biol. 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Progr, 17, pp. 1128-1136Gaspani, L., Limiroli, E., Ferrario, P., Bianchi, M., In vivo and in vitro effects of bromelain on PGE(2) and SP concentrations in the inflammatory exudate in rats (2002) Pharmacology, 65, pp. 83-86Haq, S.K., Rasheedi, S., Khan, R.H., Characterization of a partially folded intermediate of stem bromelain at low pH (2002) Eur. J. Biochem, 269, pp. 47-52Harrach, T., Eckert, K., Maurer, H.R., Machleidt, I., Machleidt, W., Nuck, R., Isolation and characterization of two forms of an acidic bromelain stem proteinase (1998) J. Protein Chem, 17, pp. 351-361Hatano, K., Sawano, Y., Tanokura, M., Structure-function relationship of bromelain isoinhibitors from pineapple stem (2002) Biol. Chem, 383, pp. 1151-1156Hatano, K., Tanokura, M., Takahashi, K., The amino acid sequences of isoforms of the bromelain inhibitor from pineapple stem (1998) J. Biochem, 124, pp. 457-461Hjorth, R., Expanded bed adsorption in industrial bioprocessing: Recent developments (1997) Trends Biotechnol, 15, p. 230Hochstrasser, D.F., Patchornik, A., Merril, C.R., Development of Polyacrylamide gels that improve the separation of proteins and their detection by silver staining (1998) Analyt. Biochem, 173, pp. 412-423Hochstrasser, D.F., Merril, C.R., Catalysts' for Polyacrylamide gel polymerization and detection of proteins by silver staining (1998) Appl. Theor. Electrophoresis, 1, pp. 35-40Hebbar, H.U., Sumana, B., Raghavarao, K.S.M.S., Use of reverse micellar systems for the extraction and purification of bromelain term from pineapple wastes (2008) Bioresource Technology, 99, pp. 4896-4902Khan, R.H., Rasheedi, S., Haq, S.K., Effect of pH, temperature and alcohols on the stability of glycosylated and deglycosylated stem bromelain (2003) J. Biosci, 28, pp. 709-714Kim, M. H., Kim, H. K., Lee, J. K., Park, S. Y., Oh, T. K., Thermostable lipase of Bacillus stearothermophilus, high level production, purification and calciumdependent thermostability. Korea Res. Inst. Biosci. Biotechnol. 64:280-286 (2000)Kordel, M., Hofmann, B., Schomburg, D., Schimid, R.D., Extracelluar lipase of Pseudomonas sp. strain ATCC 21808: Purification, characterization, crystallization and preliminary X-ray diffraction data (1991) J. Bacteriol, 173, pp. 4836-4841Kunitz, M., Crystalline soybean trypsin inhibitor: II general properties (1974) J. Gen. Physiol, 30, pp. 291-310Lali, A.M., Khare, A.S., Joshi, J.B., Behaviour of solid particles in viscous non-newtonian solutions: Settling velocity, wall effects and bed expansion in solid-liquid fluidized beds (1989) Powder Tech, 57, pp. 39-50Maurer, H.R., Bromelain: Biochemistry, pharmacology and medical use (2001) Cell. Mol. Life Sci, 58, pp. 1234-1245Mullick, A., Flickinger, M.C., Expanded bed adsorption of human serum albumin from very dense saccharomyces cerevesiae suspensions on fluoride-modified zirconia (1999) Biotechnol. Bioeng, 65, pp. 282-290Murachi, T., Bromelain enzymes (1976) Methods Enzymol, 45, pp. 475-485Ota, S., Horie, K., Hagino, F., Hashimoto, C., Date, H., Fractionation and some properties of the proteolytically active components of bromelains in the stem and the fruit of the pineapple plant (1972) J. Biochem, 71, pp. 817-830Rasheedi, S., Haq, S.K., Khan, R.H., Guanidine hydrochloride denaturation of glycosylated and deglycosylated stem bromelain (2003) Biochemistry, 68, pp. 1097-1100Roy, I., Pai, A., Lali, A., Gupta, M.N., Comparison of batch, packed bed and expanded bed purification of A. niger cellulose beads (1999) Bioseparation, 8, pp. 317-326Santos, E.S., Guirardello, R., Franco, T.T., Distributor Effect on Expanded Bed Adsorption (2000) International Conference IEX 2000 (Ion Exchange at the Millennium), , Cambridge/UKSantos, E.S., Guirardello, R., Franco, T.T., Preparative chromatography of xylanase using expanded bed adsorption (2002) J. Chrom. A, 944, pp. 217-224Silveira, E., (2007) Purificação e caracterização de bromelina a partir do extrato bruto de Ananas comosus por adsorção em leito expandido, , Dissertação de Mestrado. Faculdade de Engenharia Química: UnicampSilverstein, R.M., Kezdy, F.J., Characterization of the pineapple stem proteases (bromelain) (1975) Arch. 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B, 846, pp. 51-56Trinh, L., Phue, J.-N., Jaluria, P., Tsai, C.W., Narum, D.L., Shiloach, J., Screen-less expanded bed column: New approach for the recovery and purification of a malaria transmission blocking vaccine candidate from Pichia pastoris (2006) Biotechnol. Lett, 28, pp. 951-958Walter, H. E., Proteinases: methods with hemoglobin, casein, and azocoll as substrates. Pp. 270-277 in Methods of Enzymatic Analysis, 5, H.U. Bergmeyer, ed. Verlag Chemie, Weinheim, Germany (1984)Wang, S.-L., Peng, J.-H., Liang, T.-W., Liu, K.-C., Purification and characterization of a chitosanase from Serratia marcescens TKU011 (2008) Carbohydrate Research, 343, pp. 1316-1323Wharton, C.W., The structure and mechanism of stem bromelain. Evaluation of the homogeneity of purified stem bromelain, determination of the molecular weight and kinetic analysis of the bromelain-catalysed hydrolysis of N-benzyloxycarbonyl-L-phenylalanyl-L-serine methyl ester (1974) Biochem. 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Recomendações Para O Tratamento Da Crise Migranosa - Um Consenso Brasileiro

In this article, a group of experts in headache management of the Brazilian Headache Society developed through a consensus strategic measurements to treat a migraine attack in both the child and the adult. Particular emphasis was laid on the treatment of migraine in women, including at pregnancy, lactation and perimenstrual period. © 2016, Associacao Arquivos de Neuro-Psiquiatria. All rights reserved.74326227

Surto de criptosporidiose em bezerros no Sul do Rio Grande do Sul

Descrevem-se os aspectos epidemiológicos, sinais clínicos e a patologia de um surto de criptosporidiose em bezerros na região Sul do Rio Grande do Sul. De um lote de 400 bezerros de 30-45 dias de idade, 35 adoeceram e 16 morreram. Os bezerros nasciam fracos e logo após o nascimento apresentavam diarreia amarela, emagrecimento progressivo, desidratação, depressão e morte entre 10 e 15 dias após o início dos sinais clínicos. Na necropsia havia congestão dos vasos sanguíneos intestinais e mesentéricos. Havia distensão intestinal por gás e dilatação de vasos linfáticos. Microscopicamente havia achatamento das vilosidades intestinais, com necrose e atrofia. Aderidas à superfície das células epiteliais das vilosidades, havia estruturas puntiformes basofílicas de 2-5µm de diâmetro compatíveis com Cryptosporidium spp. A microscopia eletrônica revelou a presença de diferentes estágios do agente aderidos às microvilosidades de enterócitos. Alerta-se para a importância da criptosporidiose como agente primário de diarreia em bezerros. São necessárias medidas preventivas no que se refere ao manejo para diminuir as perdas econômicas e a contaminação ambiental, e, ainda, diminuir o risco para a saúde pública

Status Report Of The Schenberg Gravitational Wave Antenna

Here we present a status report of the Schenberg antenna. In the past three years it has gone to a radical upgrading operation, in which we have been installing a 1K pot dilution refrigerator, cabling and amplifiers for nine transducer circuits, designing a new suspension and vibration isolation system for the microstrip antennas, and developing a full set of new transducers, microstrip antennas, and oscillators. We are also studying an innovative approach, which could transform Schenberg into a broadband gravitational wave detector.3631Aguiar, O.D., (2002) Class. Quantum Grav., 19, p. 1949Aguiar, O.D., (2004) Class. Quantum Grav., 21, pp. S457Aguiar, O.D., (2005) Class. Quantum Grav., 22, pp. S209Aguiar, O.D., (2006) Class. Quantum Grav., 23, pp. S239Aguiar, O.D., (2008) Class. Quantum Grav., 25, p. 114042Costa, C.A., (2008) Class. Quantum Grav., 25, p. 184002Johnson, W.W., Merkowitz, S.M., (1993) Phys. Rev. Lett., 70, p. 2367Coccia, E., Lobo, J.A., Ortega, J.A., (1995) Phys. Rev. D, 52, p. 3735Thorne, K.S., (1978) Phys. Rev. Lett., 40, p. 667Tobar, M.E., Ivanov, E.N., Blair, D.G., (2000) Gen. Rel. Grav., 32, p. 1799De Waard, (2005) Class. Quantum Grav., 22, pp. S215Vinet, J.-Y., (2010) Research in Astron Astrophys., 10, p. 956Costa, C.A., Aguiar, O.D., Magalhães, N.S., (2004) Class. Quantum Grav., 21, pp. S827Forward, R.L., (1971) Gen. Rel. Grav., 2, p. 149Eardley, D.M., Lee, D.L., Lightman, A.P., Wagoner, R.V., Will, C.M., (1973) Phys. Rev. Lett., 30, p. 884Bianchi, M., Coccia, E., Colacino, C.N., Fafone, V., Fucito, F., (1996) Class. Quantum Grav., 13, p. 2865Andrade, L.A., (2009) Microwave and Optical Tech. Lett., 51, p. 1120Furtado, S.R., (2012), in preparationIvanov, E.N., Hartnett, J.G., Tobar, M.E., (2000) IEEE Trans. Ultrason., Ferroelect., Freq. Contr., 47, p. 1526Pimentel, G.L., (2008) J. Phys. Conf. Series, 122, p. 012028Aguiar, (2009) Int. J. Modern Phys. D, 18, p. 2317Furtado, S.R., (2009), Ph.D. Thesis at INPE, not publishedBraginsky, V.B., Vorontsov, Y.I., Thorne, K.S., (1980) Science, 209, p. 547Thorne, K.S., The Quantum Limit for Gravitational-Wave Detectors and Methods of Circumventing It (1979) Sources of Gravitational Waves, p. 49. , ed. L L Smarr, Cambridge University Press, Cambridge, US