Microalgae Schizochytrium sp. in diets for Piau juvenilles

Todos os textos, informa??es e resultados apresentados s?o de inteira responsabilidade dos autores.O DHA ? um ?cido importante da s?rie ?mega 3, fundamental para a forma??o do tecido nervoso e visual em humanos, e confere tamb?m uma boa forma??o da massa encef?lica. O presente trabalho teve o objetivo de avaliar dietas contendo a inclus?o da alga Schizochytrium sp. no desempenho produtivo de juvenis de piau.O experimento foi conduzido no Laborat?rio de Aquicultura e Ecologia Aqu?tica do Departamento de Zootecnia da Universidade Federal dos Vales do Jequitinhonha e Mucuri, em Diamantina MG. Foram utilizados 300 juvenis de Piau (Leporinus friderici) com peso e comprimento total m?dio de 11,8 e 9,68 g respectivamente . Os peixes passaram por sete dias de adapta??o e foram alimentados com a ra??o do tratamento controle. . O experimento foi realizado em um delineamento inteiramente casualizado, com cinco tratamentos (0, 10, 20, 30, e 40 g de Schizochytrium sp/kg-1)e quatro repeti??es. Sete juvenis foram estocados por aqu?rio, totalizando 20 parcelas experimentais. Avaliou-se aos 60 dias de experimento par?metros de desempenho produtivo. Foram mensurados: comprimento padr?o (cm), comprimento total (cm), ganho de peso (g), consumo de ra??o (g), convers?o alimentar (g/g)?, biomassa final (g), e sobreviv?ncia (%). As vari?veis de desempenho apresentaram melhores resultados de acordo com o aumento do n?vel de inclus?o da alga Schizochytrium sp em juvenis de piau. Conlcui-se com este estudo que juvenis de piau alimentados com 40g de Schizochytrium sp.kg-1 apresentam melhor desempenho produtivo e sobreviv?ncia.DHA is an important acid of the omega-3 series, essential for the formation of the nervous and visual tissue in humans, and also gives a good formation of brain matter. This study aimed to evaluate diets with the inclusion of algae Schizochytrium sp. In productive performance of piau juveniles.. Experiment was conducted at the Aquaculture Laboratory of Aquatic Ecology of the Department of Animal Science, of the Federal University of the Jequitinhonha and Mucuri Valleys, in Diamantina MG. . Three hundred Piau (Leporinus friderici) juvenile, with body weight and length means of 11.8, and 9.68 g respectively. Fish have undergone seven days of adaptation and were fed with the control ration treatment. The experiment was conducted in a completely randomized design, with five treatments (0, 10, 20, 30, and 40 g of Schizochytrium sp. Kg-1) and four replications. Seven juveniles were stocked per aquarium, totaling 20 experimental plots. It was evaluated at 60 days of the experiment For the productive performance were measured: standard length (cm), total length (cm), weight gain (g), feed intake (g), feed conversion (g / g) ? , final biomass (g) EC (%) and survival (%). The performance variables showed greater results in accordance with the increase in the level of inclusionof Schizochytrium sp in piau juveniles diets. It follows that piau juveniles fed with 40g Schizochytrium sp.kg-1 have greater growth performance and survival

Relationship arachidonic acid (ARA): docosahexaenoic acid (DHA) in the body composition of Nile tilapia fed with diets containing the microalgae Schizochytrium sp.

Todos os textos, informa??es e resultados apresentados s?o de inteira responsabilidade dos autores.Ag?ncias financiadoras para a realiza??o do trabalho: Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq), Alltech Inc., Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) e Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais (FAPEMIG).O ?cido araquid?nico compete com DHA pelas mesmas vias metab?licas na forma??o de membranas e constitui??o das c?lulas do f?gado. Isto sugere que a ingest?o de DHA podem substituir parcialmente o ?cido araquid?nico atrav?s da modifica??o da composi??o de ?cidos graxos nos tecidos, o que reduz a ocorr?ncia de desordens nos sitema imune e doen?as inflamat?rias. Com isso, objetivou-se avaliar a rela??o ARA : DHA corporal de alevinos de til?pia do Nilo alimentadas com ra??es contendo a microalga Schizochytrium sp..Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq)Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais (FAPEMIG)The arachidonic acid competes with DHA by the same pathways in the formation of membranes and formation of liver cells. This suggests that intake of DHA may partially replace arachidonic acid by modifying the composition of fatty acids in tissues, reducing the occurrence of immune system disorders and inflammatory diseases. The relationship ARA: DHA body of Nile tilapia fry fed diets containing the microalgae Schizochytrium sp. was evaluated

Alternative splicing enriched cDNA libraries identify breast cancer-associated transcripts

<p>Abstract</p> <p>Background</p> <p>Alternative splicing (AS) is a central mechanism in the generation of genomic complexity and is a major contributor to transcriptome and proteome diversity. Alterations of the splicing process can lead to deregulation of crucial cellular processes and have been associated with a large spectrum of human diseases. Cancer-associated transcripts are potential molecular markers and may contribute to the development of more accurate diagnostic and prognostic methods and also serve as therapeutic targets. Alternative splicing-enriched cDNA libraries have been used to explore the variability generated by alternative splicing. In this study, by combining the use of trapping heteroduplexes and RNA amplification, we developed a powerful approach that enables transcriptome-wide exploration of the AS repertoire for identifying AS variants associated with breast tumor cells modulated by <it>ERBB2</it> (<it>HER-2/neu</it>) oncogene expression.</p> <p>Results</p> <p>The human breast cell line (C5.2) and a pool of 5 ERBB2 over-expressing breast tumor samples were used independently for the construction of two AS-enriched libraries. In total, 2,048 partial cDNA sequences were obtained, revealing 214 alternative splicing sequence-enriched tags (ASSETs). A subset with 79 multiple exon ASSETs was compared to public databases and reported 138 different AS events. A high success rate of RT-PCR validation (94.5%) was obtained, and 2 novel AS events were identified. The influence of <it>ERBB2</it>-mediated expression on AS regulation was evaluated by capillary electrophoresis and probe-ligation approaches in two mammary cell lines (Hb4a and C5.2) expressing different levels of <it>ERBB2</it>. The relative expression balance between AS variants from 3 genes was differentially modulated by <it>ERBB2</it> in this model system.</p> <p>Conclusions</p> <p>In this study, we presented a method for exploring AS from any RNA source in a transcriptome-wide format, which can be directly easily adapted to next generation sequencers. We identified AS transcripts that were differently modulated by <it>ERBB2</it>-mediated expression and that can be tested as molecular markers for breast cancer. Such a methodology will be useful for completely deciphering the cancer cell transcriptome diversity resulting from AS and for finding more precise molecular markers.</p

The Genomic Ancestry of Individuals from Different Geographical Regions of Brazil Is More Uniform Than Expected

Based on pre-DNA racial/color methodology, clinical and pharmacological trials have traditionally considered the different geographical regions of Brazil as being very heterogeneous. We wished to ascertain how such diversity of regional color categories correlated with ancestry. Using a panel of 40 validated ancestry-informative insertion-deletion DNA polymorphisms we estimated individually the European, African and Amerindian ancestry components of 934 self-categorized White, Brown or Black Brazilians from the four most populous regions of the Country. We unraveled great ancestral diversity between and within the different regions. Especially, color categories in the northern part of Brazil diverged significantly in their ancestry proportions from their counterparts in the southern part of the Country, indicating that diverse regional semantics were being used in the self-classification as White, Brown or Black. To circumvent these regional subjective differences in color perception, we estimated the general ancestry proportions of each of the four regions in a form independent of color considerations. For that, we multiplied the proportions of a given ancestry in a given color category by the official census information about the proportion of that color category in the specific region, to arrive at a “total ancestry” estimate. Once such a calculation was performed, there emerged a much higher level of uniformity than previously expected. In all regions studied, the European ancestry was predominant, with proportions ranging from 60.6% in the Northeast to 77.7% in the South. We propose that the immigration of six million Europeans to Brazil in the 19th and 20th centuries - a phenomenon described and intended as the “whitening of Brazil” - is in large part responsible for dissipating previous ancestry dissimilarities that reflected region-specific population histories. These findings, of both clinical and sociological importance for Brazil, should also be relevant to other countries with ancestrally admixed populations