A study of CP violation in B-+/- -> DK +/- and B-+/- -> D pi(+/-) decays with D -> (KSK +/-)-K-0 pi(-/+) final states

A first study of CP violation in the decay modes $B^\pm\to [K^0_{\rm S} K^\pm \pi^\mp]_D h^\pm$ and $B^\pm\to [K^0_{\rm S} K^\mp \pi^\pm]_D h^\pm$, where $h$ labels a $K$ or $\pi$ meson and $D$ labels a $D^0$ or $\overline{D}^0$ meson, is performed. The analysis uses the LHCb data set collected in $pp$ collisions, corresponding to an integrated luminosity of 3 fb$^{-1}$. The analysis is sensitive to the CP-violating CKM phase $\gamma$ through seven observables: one charge asymmetry in each of the four modes and three ratios of the charge-integrated yields. The results are consistent with measurements of $\gamma$ using other decay modes

Measurement of Upsilon production in collisions at root s=2.76 TeV

The production of $\Upsilon(1S)$, $\Upsilon(2S)$ and $\Upsilon(3S)$ mesons decaying into the dimuon final state is studied with the LHCb detector using a data sample corresponding to an integrated luminosity of 3.3 $pb^{-1}$ collected in proton-proton collisions at a centre-of-mass energy of $\sqrt{s}=2.76$ TeV. The differential production cross-sections times dimuon branching fractions are measured as functions of the $\Upsilon$ transverse momentum and rapidity, over the ranges $p_{\rm T} Upsilon(1S) X) x B(Upsilon(1S) -> mu+mu-) = 1.111 +/- 0.043 +/- 0.044 nb, sigma(pp -> Upsilon(2S) X) x B(Upsilon(2S) -> mu+mu-) = 0.264 +/- 0.023 +/- 0.011 nb, sigma(pp -> Upsilon(3S) X) x B(Upsilon(3S) -> mu+mu-) = 0.159 +/- 0.020 +/- 0.007 nb, where the first uncertainty is statistical and the second systematic

Study of forward Z + jet production in pp collisions at √s=7 TeV

A measurement of the $Z(\rightarrow\mu^+\mu^-)$+jet production cross-section in $pp$ collisions at a centre-of-mass energy $\sqrt{s} = 7$ TeV is presented. The analysis is based on an integrated luminosity of $1.0\,\text{fb}^{-1}$ recorded by the LHCb experiment. Results are shown with two jet transverse momentum thresholds, 10 and 20 GeV, for both the overall cross-section within the fiducial volume, and for six differential cross-section measurements. The fiducial volume requires that both the jet and the muons from the Z boson decay are produced in the forward direction ($2.0<\eta<4.5$). The results show good agreement with theoretical predictions at the second-order expansion in the coupling of the strong interaction.A measurement of the $Z(\rightarrow\mu^+\mu^-)$+jet production cross-section in $pp$ collisions at a centre-of-mass energy $\sqrt{s} = 7$ TeV is presented. The analysis is based on an integrated luminosity of $1.0\,\text{fb}^{-1}$ recorded by the LHCb experiment. Results are shown with two jet transverse momentum thresholds, 10 and 20 GeV, for both the overall cross-section within the fiducial volume, and for six differential cross-section measurements. The fiducial volume requires that both the jet and the muons from the Z boson decay are produced in the forward direction ($2.0<\eta<4.5$). The results show good agreement with theoretical predictions at the second-order expansion in the coupling of the strong interaction

Polimorfismos da região promotora do gene apoM em crianças com diabetes mellitus tipo 1

Orientadora : Profª. Drª. Fabiane G. de M. RegoCoorientador : Prof. Dr. Geraldo PichethDissertação (mestrado) - Universidade Federal do Paraná, Setor de Ciências da Saúde, Programa de Pós-Graduação em Ciências Farmacêuticas. Defesa: Curitiba, 15/12/2016Inclui referências : f. [85-98]Área de concentração: Análises clínicasResumo: O Diabetes mellitus (DM) atualmente considerada uma epidemia, é um grupo heterogêneo de doenças metabólicas caracterizada por hiperglicemia, resultante de defeitos na secreção ou na ação da insulina. Em crianças e adolescentes a forma mais comum de diabetes é o tipo 1 (DM1), que pode corresponder até 10% dos casos de DM. O DM1 se desenvolve como consequência de uma combinação de predisposição genética, associados a fatores ambientais levando à destruição das células ?, produtoras de insulina. O diagnóstico precoce, o bom controle glicêmico e lipídico, além de tratamento adequado minimizam as comorbidades associadas à doença. O objetivo deste trabalho foi estudar variações genéticas da região promotora do gene apoM associadas ao DM1, correlacionando com biomarcadores de controle glicêmico. O projeto foi aprovado pelo Comitê de Ética em Pesquisa da Universidade Federal do Paraná - Setor de Ciências da Saúde (CAAE: 24676613.6.0000.0102). Neste estudo 317 crianças com idade de até 14 anos foram divididas em dois grupos: o de crianças controle (n=169), com indivíduos saudáveis e o grupo DM1 (n=148) com indivíduos doentes. Foram quantificados biomarcadores de controle glicêmico, perfil lipídico e de função renal. Os altos valores encontrados para HbA1c (9,7%) e baixos para o 1,5-AG (2,9 ?g/mL), caracterizam o mau controle glicêmico encontrado no grupo DM1. Embora as concentrações do colesterol total, HDL-c, LDL-c e triglicérides tenham sido estatisticamente diferentes entre os grupos (P <0,001) permanecem dentro dos intervalos de referência para os respectivos parâmetros analisados, não caracterizando os indivíduos como dislipidêmicos. Para os biomarcadores de função renal as diferenças entre os grupos tiveram um P<0,001, com exceção da albumina, porém os indivíduos não foram declarados com qualquer tipo de lesão renal até o fim deste estudo. Os polimorfismos da região promotora do gene apoM foram genotipados para ambos os grupos, sendo utilizadas as técnicas de PCR-RFLP para os polimorfismos rs805296 e rs9404941 e a de PCR em tempo real com sonda TaqMan® para o rs805297. Todos os polimorfismos analisados estão no equilíbrio de Hardy-Weinberg. Neste estudo os polimorfismos rs805296 (P=0,142) e rs9404941 (P=0,142) não foram associados ao DM1 quando analisados no modelo co-dominante. As frequências para os alelos raros dos polimorfismos em estudo foram, no geral, similares aos descritos para outras populações europeias ou Caucasoides e menores quando comparados a Orientais. O alelo raro (C) do polimorfismo rs9404941 foi associado ao aumento das concentrações de glicemia ao acaso (P=0,011) e diminuição da concentração de LDL-colesterol apenas em crianças portadoras de DM1, não havendo relatos anteriores da associação entre este polimorfismo com ambos os parâmetros bioquímicos para pacientes diabéticos. O rs805297 quando analisado no modelo recessivo mostrou diferença estatística entre os grupos (P=0,021), sugerindo associação do alelo A como sendo protetor à doença, embora novos estudos com diferentes populações e maior tamanho amostral devam ser realizados para substanciar os resultados encontrados. Palavras-chave: Diabetes mellitus, polimorfismo, gene apoM.Abstract: Diabetes mellitus (DM) actualy considered an epidemic is a heterogeneous group of metabolic diseases characterized by hyperglycemia resulting from defects in secretion or insulin action. In children and adolescents the most common form of diabetes is type 1 (DM1), which may correspond to 10% of cases of diabetes. Develops DM1 as a result of a combination of genetic predisposition, environmental factors associated with leading to the destruction of ? cells produce insulin. Early diagnosis, glycemic and lipid control appropriate, and proper treatment minimizes the comorbidities associated with the disease. The objective of this work was to study genetic variations in the promoter region of apoM gene associated DM1, correlating with biomarkers glycemic control. The project was approved by the Ethics Committee of the Federal University of Paraná - Health Sciences Sector (CAAE: 24676613.6.0000.0102). This study 317 children aged up to 14 years, were divided into two groups: control children (n=169), with healthy subjects and DM1 group (n=148) with diseased individuals. Were measured biomarkers glycemic control, lipid profile and renal function. The high values found for HbA1c (9.7%) and lower for 1,5- AG (2.9 mg/mL) characterize poor glycemic control found in DM1 group. Though the concentrations of total cholesterol, HDL-c, LDL-c and triglycerides were statistically different between groups (P <0.001) remain within reference ranges for the respective analysis parameters no featuring as dyslipidemic individuals. To biomarkers of renal function differences between groups had a P <0.001, with the exception of albumin, but the subjects were not reported in any type of renal injury until the end of the study. The polymorphisms of the promoter region of gene apoM were genotyped both groups being used PCR-RFLP techniques to rs805296 and rs9404941 polymorphisms and real-time PCR with TaqMan probe to rs805297. All polymorphisms analyzed in Hardy-Weinberg equilibrium. This study rs805296 (P=0.142) and rs9404941 (P = 0.142) polymorphisms, were not associated with DM1 when analyzed in co-dominant model. The frequencies to the rare alleles of polymorphisms were generally similar to described for other European or Caucasoid populations and lowers to compared Eastern. The rare allele (C) rs9404941 polymorphism was associated with increased glucose concentrations random (P = 0.011) and decrease LDL-cholesterol concentration only in children DM1 with no previous reports of the association between this polymorphism with both biochemical parameters for diabetic patients. The rs805297 when analyzed in the recessive model demonstrate statistical differences between groups (P = 0.021), that suggest association of the allele to be protector to disease, although further studies with different populations and increased sample amount should be conducted to prove results. Keywords: Diabetes mellitus, polymorphism, gene apoM

Evaluation of 1,5-Anhydroglucitol as a Biomarker for Type 2 Diabetes Mellitus in Patients without Overt Nephropathy

1,5-Anhydroglucitol (1,5-AG) is a non-fasting glycemic marker that responds to hyperglycemia excursions. The reduction in serum levels of 1,5-AG is associated with an increase in postprandial glycemia and glycosuria, phenomena that increase the risk and severity of diabetic complications. The objective is to assess the ability of 1,5-AG to discriminate type 2 diabetes (T2D) patients without overt kidney disease, for screening or diagnostic purposes. The Human Research Ethics Committee of Universidade Federal do Paraná (UFPR) approved the project. Serum samples from 567 individuals classified as healthy subjects (n = 291) and T2D (n = 276) with moderate glycemic control (HbA1c of 7-8%), matched by gender, were analyzed. Serum 1,5-AG levels were measured using an automated enzymatic method (GlycoMark, Inc.). Receiver Operating Characteristic (ROC) curve analysis for 1,5- AG showed sensibility of 65.3% and specificity of 91.1% to detect T2D at cut-off point of 92 µmol/L. The results were similar to the groups’ discrimination by glycemia (sensibility/specificity, 62.2%; 89.0%) at cut-off point of 6.3 mmol/L. HbA1c was the best discriminator (sensibility/specificity, 87.4%; 94.2%) at a cut-off point of 5.8% (40 mmol/mol). The serum 1,5-AG concentration was not able to discriminate T2D in the presence of moderate glycemic control with no overt nephropathy

Observation of associated production of a ZZ boson with a DD meson in the forward region

A search for associated production of a $Z$ boson with an open charm meson is presented using a data sample, corresponding to an integrated luminosity of $1.0\mathrm{fb}^{-1}$ of proton--proton collisions at a centre-of-mass energy of 7 TeV, collected by the LHCb experiment. Seven candidate events for associated production of a $Z$ boson with a $D^0$ meson and four candidate events for a $Z$ boson with a $D^+$ meson are observed with a combined significance of 5.1 standard deviations. The production cross-sections in the forward region are measured to be $$\sigma_{Z\rightarrow\mu^+\mu^-\!,D^0} = 2.50\pm1.12\pm0.22pb$$ $$\sigma_{Z\rightarrow\mu^+\mu^-\!,D^+} = 0.44\pm0.23\pm0.03pb,$$ where the first uncertainty is statistical and the second systematic

Updated measurements of exclusive J/ψJ/\psi and ψ(2S)\psi(2S) production cross-sections in pppp collisions at s=7\sqrt{s}=7 TeV

The differential cross-section as a function of rapidity has been measured for the exclusive production of $J/\psi$ and $\psi(2S)$ mesons in proton-proton collisions at $\sqrt{s}=7$ TeV, using data collected by the LHCb experiment, corresponding to an integrated luminosity of 930 pb$^{-1}$. The cross-sections times branching fractions to two muons having pseudorapidities between 2.0 and 4.5 are measured to be $$\begin{array}{rl} \sigma_{pp\rightarrow J/\psi\rightarrow{\mu^+}{\mu^-}}(2.0<\eta_{\mu^\pm }<4.5)=&291\pm 7\pm19 {\rm \ pb},\\ \sigma_{pp\rightarrow\psi(2S)\rightarrow{\mu^+}{\mu^-}}(2.0<\eta_{\mu^\pm}<4.5)=&6.5\pm 0.9\pm 0.4 {\rm \ pb},\end{array}$$ where the first uncertainty is statistical and the second is systematic. The measurements agree with next-to-leading order QCD predictions as well as with models that include saturation effects.The differential cross-section as a function of rapidity has been measured for the exclusive production of $J/\psi$ and $\psi(2S)$ mesons in proton-proton collisions at $\sqrt{s}=7$ TeV, using data collected by the LHCb experiment, corresponding to an integrated luminosity of 930 pb$^{-1}$. The cross-sections times branching fractions to two muons having pseudorapidities between 2.0 and 4.5 are measured to be $$\begin{array}{rl} \sigma_{pp\rightarrow J/\psi\rightarrow{\mu^+}{\mu^-}}(2.0<\eta_{\mu^\pm }<4.5)=&291\pm 7\pm19 {\rm \ pb},\\ \sigma_{pp\rightarrow\psi(2S)\rightarrow{\mu^+}{\mu^-}}(2.0<\eta_{\mu^\pm}<4.5)=&6.5\pm 0.9\pm 0.4 {\rm \ pb},\end{array}$$ where the first uncertainty is statistical and the second is systematic. The measurements agree with next-to-leading order QCD predictions as well as with models that include saturation effects.The differential cross-section as a function of rapidity has been measured for the exclusive production of $J/\psi$ and $\psi(2S)$ mesons in proton-proton collisions at $\sqrt{s}=7$ TeV, using data collected by the LHCb experiment, corresponding to an integrated luminosity of 930 pb$^{-1}$. The cross-sections times branching fractions to two muons having pseudorapidities between 2.0 and 4.5 are measured to be $$\begin{array}{rl} \sigma_{pp\rightarrow J/\psi\rightarrow{\mu^+}{\mu^-}}(2.0<\eta_{\mu^\pm }<4.5)=&291\pm 7\pm19 {\rm \ pb},\\ \sigma_{pp\rightarrow\psi(2S)\rightarrow{\mu^+}{\mu^-}}(2.0<\eta_{\mu^\pm}<4.5)=&6.5\pm 0.9\pm 0.4 {\rm \ pb},\end{array}$$ where the first uncertainty is statistical and the second is systematic. The measurements agree with next-to-leading order QCD predictions as well as with models that include saturation effects

Measurement of the Bc+B_c^+ meson lifetime using Bc+→J/ψμ+νμXB_c^+ \to J/\psi\mu^+ \nu_{\mu} X decays

The lifetime of the $B_c^+$ meson is measured using semileptonic decays having a $J/\psi$ meson and a muon in the final state. The data, corresponding to an integrated luminosity of $2\mathrm{~fb^{-1}}$, are collected by the LHCb detector in $pp$ collisions at a centre-of-mass energy of $8\,\mathrm{TeV}$. The measured lifetime is $$\tau = 509 \pm 8 \pm 12 \mathrm{~fs},$$ where the first uncertainty is statistical and the second is systematic.The lifetime of the $B_c^+$ meson is measured using semileptonic decays having a $J\!/\!\psi$ meson and a muon in the final state. The data, corresponding to an integrated luminosity of $2\mathrm{~fb^{-1}}$, are collected by the LHCb detector in $pp$ collisions at a centre-of-mass energy of $8\,\mathrm{TeV}$. The measured lifetime is $$\tau = 509 \pm 8 \pm 12 \mathrm{~fs},$$ where the first uncertainty is statistical and the second is systematic.The lifetime of the B c + meson is measured using semileptonic decays having a J / ψ meson and a muon in the final state. The data, corresponding to an integrated luminosity of 2 fb - 1 , are collected by the LHCb detector in p p collisions at a centre-of-mass energy of 8 TeV. The measured lifetime is τ = 509 ± 8 ± 12 fs , where the first uncertainty is statistical and the second is systematic

Studies of beauty baryon decays to D0ph−D^0 ph^- and Λc+h−\Lambda_c^+ h^- final states

Decays of beauty baryons to the $D^0 p h^-$ and $\Lambda_c^+ h^-$ final states (where $h$ indicates a pion or a kaon) are studied using a data sample of $pp$ collisions, corresponding to an integrated luminosity of 1.0 fb$^{-1}$, collected by the LHCb detector. The Cabibbo-suppressed decays $\Lambda_b^0\to D^0 p K^-$ and $\Lambda_b^0\to \Lambda_c^+ K^-$ are observed and their branching fractions are measured with respect to the decays $\Lambda_b^0\to D^0 p \pi^-$ and $\Lambda_b^0\to \Lambda_c^+ \pi^-$. In addition, the first observation is reported of the decay of the neutral beauty-strange baryon $\Xi_b^0$ to the $D^0 p K^-$ final state, and a measurement of the $\Xi_b^0$ mass is performed. Evidence of the $\Xi_b^0\to \Lambda_c^+ K^-$ decay is also reported.Decays of beauty baryons to the D0ph− and Λc+h− final states (where h indicates a pion or a kaon) are studied using a data sample of pp collisions, corresponding to an integrated luminosity of 1.0  fb−1, collected by the LHCb detector. The Cabibbo-suppressed decays Λb0→D0pK− and Λb0→Λc+K− are observed, and their branching fractions are measured with respect to the decays Λb0→D0pπ− and Λb0→Λc+π−. In addition, the first observation is reported of the decay of the neutral beauty-strange baryon Ξb0 to the D0pK− final state, and a measurement of the Ξb0 mass is performed. Evidence of the Ξb0→Λc+K− decay is also reported.Decays of beauty baryons to the $D^0 p h^-$ and $\Lambda_c^+ h^-$ final states (where $h$ indicates a pion or a kaon) are studied using a data sample of $pp$ collisions, corresponding to an integrated luminosity of 1.0 fb$^{-1}$, collected by the LHCb detector. The Cabibbo-suppressed decays $\Lambda_b^0\to D^0 p K^-$ and $\Lambda_b^0\to \Lambda_c^+ K^-$ are observed and their branching fractions are measured with respect to the decays $\Lambda_b^0\to D^0 p \pi^-$ and $\Lambda_b^0\to \Lambda_c^+ \pi^-$. In addition, the first observation is reported of the decay of the neutral beauty-strange baryon $\Xi_b^0$ to the $D^0 p K^-$ final state, and a measurement of the $\Xi_b^0$ mass is performed. Evidence of the $\Xi_b^0\to \Lambda_c^+ K^-$ decay is also reported