UV laser mediated cell selective destruction by confocal microscopy

Analysis of cell-cell interactions, cell function and cell lineages greatly benefits selective destruction techniques, which, at present, rely on dedicated, high energy, pulsed lasers and are limited to cells that are detectable by conventional microscopy. We present here a high resolution/sensitivity technique based on confocal microscopy and relying on commonly used UV lasers. Coupling this technique with time-lapse enables the destruction and following of any cell(s) in any pattern(s) in living animals as well as in cell culture systems

Plant Insecticide L-Canavanine Repels Drosophila via the Insect Orphan GPCR DmX

An orphan G-protein-coupled gustatory receptor mediates detection of the plant poison L-canavanine in fruit flies

Un nouveau procédé de cystostomie continente avec iléocystoplastie d'agrandissement (à propos d'une série rétrospective de 32 patients)

MONTPELLIER-BU Médecine UPM (341722108) / SudocPARIS-BIUM (751062103) / SudocMONTPELLIER-BU Médecine (341722104) / SudocSudocFranceF

Enregistrement histopathologique des tumeurs urologiques en Languedoc-Roussillon de 1999 à 2000

MONTPELLIER-BU Médecine UPM (341722108) / SudocPARIS-BIUM (751062103) / SudocMONTPELLIER-BU Médecine (341722104) / SudocSudocFranceF

Identification des transporteurs d'acides aminés excitateurs chez la Drosophile et analyse de leurs profils d'expression au cours du développement

AIX-MARSEILLE2-BU Sci.Luminy (130552106) / SudocSudocFranceF

Évaluation du système ACT® (Adjustable Continence Therapy) pour le traitement de l'incontinence urinaire d'effort féminine par insuffisance sphinctérienne

MONTPELLIER-BU Médecine UPM (341722108) / SudocMONTPELLIER-BU Médecine (341722104) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Control of gcm RNA stability is necessary for proper glial cell fate acquisition.

The control of RNA stability is an important post-transcriptional event. While neural development is known to require proteins that bind AU-rich elements (ARE) and affect RNA half-life, the role of specific RNA stability in this process remains elusive. In the Drosophila embryo, glial fate acquisition is triggered by glial cells missing (gcm) master gene, which is transiently expressed in all gliogenic stem cells and submitted to tight transcriptional regulation. By using in vitro and in vivo site directed mutagenesis, we have discovered that gcm RNA is unstable and that its 3'UTR confers labile properties to RNA due to the presence of an ARE motif. Moreover, we show that the gliogenic potential of Gcm transcription factor increases when ARE is abolished and demonstrate the importance of gcm RNA stability in the acquisition of the glial fate. Thus, control of a single RNA half-life is crucial for nervous system differentiation

UV laser mediated cell selective destruction by confocal microscopy

Abstract Analysis of cell-cell interactions, cell function and cell lineages greatly benefits selective destruction techniques, which, at present, rely on dedicated, high energy, pulsed lasers and are limited to cells that are detectable by conventional microscopy. We present here a high resolution/sensitivity technique based on confocal microscopy and relying on commonly used UV lasers. Coupling this technique with time-lapse enables the destruction and following of any cell(s) in any pattern(s) in living animals as well as in cell culture systems.</p