12 research outputs found
Estudio de los procesos de plasticidad a largo plazo en el hipocampo dorsal: papel del Canal Girk en modelos amiloidosis
La hiperexcitabilidad hipocampal, acontecimiento que se observa de manera temprana en la enfermedad de Alzheimer (EA), puede afectar a la generación de procesos de plasticidad a largo plazo fundamentales para la formación de memorias y el aprendizaje, capacidades cognitivas que se ven gravemente deterioradas en esta patología. En este escenario, los canales de K+ rectificadores de entrada acoplados a proteína-G (GirK), esenciales para el control de la excitabilidad neuronal en el hipocampo dorsal, podrían verse afectados durante la patogénesis de la EA. Por esta razón, estrategias dirigidas hacia el aumento de la actividad inhibitoria de los canales GirK, con el fin de restaurar el balance excitación/inhibición hipocampal necesario para el desarrollo adecuado de los procesos de plasticidad sináptica, podrían suponer un abordaje prometedor para la prevención de los déficits cognitivos asociados a esta enfermedad.
En la presente tesis doctoral, hemos examinado el papel de la señalización dependiente de los canales GirK en los procesos de plasticidad a largo plazo en hipocampo dorsal de ratón. Asimismo, hemos estudiado la relación existente entre los canales GirK y la disfunción hipocampal originada por la patología amiloide, así como la posibilidad de modular su actividad con el fin de revertir las anomalías sinápticas y cognitivas que se observan en la EA.
Para alcanzar este objetivo, hemos realizado un abordaje multidisciplinar utilizando modelos de amiloidosis generados por la administración de formas oligoméricas solubles del péptido A 1-42. Tras la modulación farmacológica de la actividad inhibitoria mediada por el canal GirK, hemos estudiado in vitro, por registros electrofisiológicos en rodajas, e in vivo, mediante pruebas de comportamiento, y posteriormente técnicas inmunohistoquímicas, la participación de los canales GirK en las alteraciones producidas por A 1-42 en la función hipocampal.
Nuestros datos revelan que, en condiciones normales, la disrupción del balance excitación/inhibición en el hipocampo dorsal por el aumento o la disminución de la transmisión inhibitoria mediada por GirK provoca alteraciones en la inducción de procesos de plasticidad a largo plazo, lo que se traduce en déficits en la memoria y el aprendizaje. Sin embargo, en una situación de hiperexcitabilidad ocasionada por A 1-42, el aumento de la señalización dependiente de GirK restaura la excitabilidad neuronal hipocampal, y con ello la generación de los procesos de plasticidad sináptica necesarios para un funcionamiento cognitivo normal. En conjunto, estos resultados evidencian la necesidad de un nivel adecuado de actividad de los canales GirK en el hipocampo dorsal para la generación de los procesos de plasticidad que subyacen a la formación de memorias y el aprendizaje, y arronjan luz sobre el empleo de estos canales como una herramienta de control de la hiperexcitabilidad que se da en las fases más tempranas de la EA a fin de restablecer la función cognitiva
PROTEOME IMAGING USING MALDI IN AN ALZHEIMER’S DISEASE MOUSE MODEL: DIFFERENTIAL EXPRESSION OF MEMORY-RELATED HIPPOCAMPAL PROTEINS IN FEMALE AND MALE MICE
SIGMA-1 RECEPTOR ACTIVATION PRESERVES HIPPOCAMPAL SYNAPTIC PLASTICITY AND HIPPOCAMPAL-DEPENDENT CONTEXTUAL AND SOCIAL MEMORY IN AN EARLY AMYLOIDOPATHY MOUSE MODEL
SYSTEMATIC CHARACTERIZATION OF A NON-TRANSGENIC AΒ1–42 AMYLOIDOSIS MODEL: SYNAPTIC PLASTICITY AND MEMORY DEFICITS IN FEMALE AND MALE MICE
VU0810464, A GIRK CHANNEL ACTIVATOR, COUNTERACTS SYNAPTIC PLASTICITY AND VISUO-SPATIAL MEMORY IMPAIRMENTS IN AN EARLY AMYLOIDOPATHY MOUSE MODEL
Systematic characterization of a non-transgenic Aβ 1–42 amyloidosis model: synaptic plasticity and memory deficits in female and male mice
Abstract Background The amyloid-β (Aβ) cascade is one of the most studied theories linked to AD. In multiple models, Aβ accumulation and dyshomeostasis have shown a key role in AD onset, leading to excitatory/inhibitory imbalance, the impairments of synaptic plasticity and oscillatory activity, and memory deficits. Despite the higher prevalence of Alzheimer’s disease (AD) in women compared to men, the possible sex difference is scarcely explored and the information from amyloidosis transgenic mice models is contradictory. Thus, given the lack of data regarding the early stages of amyloidosis in female mice, the aim of this study was to systematically characterize the effect of an intracerebroventricular (icv.) injection of Aβ 1–42 on hippocampal-dependent memory, and on associated activity-dependent synaptic plasticity in the hippocampal CA1–CA3 synapse, in both male and female mice. Methods To do so, we evaluated long term potentiation (LTP) with ex vivo electrophysiological recordings as well as encoding and retrieval of spatial (working, short- and long-term) and exploratory habituation memories using Barnes maze and object location, or open field habituation tasks, respectively. Results Aβ 1–42 administration impaired all forms of memory evaluated in this work, regardless of sex. This effect was displayed in a long-lasting manner (up to 17 days post-injection). LTP was inhibited at a postsynaptic level, both in males and females, and a long-term depression (LTD) was induced for the same prolonged period, which could underlie memory deficits. Conclusions In conclusion, our results provide further evidence on the shifting of LTP/LTD threshold due to a single icv. Aβ 1–42 injection, which underly cognitive deficits in the early stages of AD. These long-lasting cognitive and functional alterations in males and females validate this model for the study of early amyloidosis in both sexes, thus offering a solid alternative to the inconsistence of amyloidosis transgenic mice models
Short-term plasticity after partial deafferentation in the oculomotor system
Medial rectus motoneurons are innervated by two main pontine inputs. The specific function of each of these two inputs remains to be fully understood. Indeed, selective partial deafferentation of medial rectus motoneurons, performed by the lesion of either the vestibular or the abducens input, initially induces similar changes in motoneuronal discharge. However, at longer time periods, the responses to both lesions are dissimilar. Alterations on eye movements and motoneuronal discharge induced by vestibular input transection recover completely 2 months post-lesion, whereas changes induced by abducens internuclear lesion are more drastic and permanent. Functional recovery could be due to some kind of plastic process, such as reactive synaptogenesis, developed by the remaining intact input, which would occupy the vacant synaptic spaces left after lesion. Herein, by means of confocal microscopy, immunocytochemistry and retrograde labeling, we attempt to elucidate the possible plastic processes that take place after partial deafferentation of medial rectus motoneuron. 48 h post-injury, both vestibular and abducens internuclear lesions produced a reduced synaptic coverage on these motoneurons. However, 96 h after vestibular lesion, there was a partial recovery in the number of synaptic contacts. This suggests that there was reactive synaptogenesis. This recovery was preceded by an increase in somatic neurotrophin content, suggesting a role of these molecules in presynaptic axonal sprouting. The rise in synaptic coverage might be due to terminal sprouting performed by the remaining main input, i.e., abducens internuclear neurons. The present results may improve the understanding of this apparently redundant input system.Ministerio de Ciencia e Innovación BFU2015-64515-
Hippocampal long-term synaptic depression and memory deficits induced in early amyloidopathy are prevented by enhancing G-protein-gated inwardly-rectifying potassium channel activity
Hippocampal synaptic plasticity disruption by amyloid-β (Aβ) peptides + thought to be responsible for learning and memory impairments in Alzheimer's disease (AD) early stage. Failures in neuronal excitability maintenance seems to be an underlying mechanism. G-protein-gated inwardly rectifying potassium (GirK) channels control neural excitability by hyperpolarization in response to many G-protein-coupled receptors activation. Here, in early in vitro and in vivo amyloidosis mouse models, we study whether GirK channels take part of the hippocampal synaptic plasticity impairments generated by Aβ1-42 . In vitro electrophysiological recordings from slices showed that Aβ1-42 alters synaptic plasticity by switching high-frequency stimulation (HFS) induced long-term potentiation (LTP) to long-term depression (LTD), which led to in vivo hippocampal-dependent memory deficits. Remarkably, selective pharmacological activation of GirK channels with ML297 rescued both HFS-induced LTP and habituation memory from Aβ1-42 action. Moreover, when GirK channels were specifically blocked by Tertiapin-Q, their activation with ML297 failed to rescue LTP from the HFS-dependent LTD induced by Aβ1-42 . On the other hand, the molecular analysis of the recorded slices by western blot showed that the expression of GIRK1/2 subunits, which form the prototypical GirK channel in the hippocampus, was not significantly regulated by Aβ1-42 . However, immunohistochemical examination of our in vivo amyloidosis model showed Aβ1-42 to down-regulate hippocampal GIRK1 subunit expression. Together, our results describe an Aβ-mediated deleterious synaptic mechanism that modifies the induction threshold for hippocampal LTP/LTD and underlies memory alterations observed in amyloidosis models. In this scenario, GirK activation assures memory formation by preventing the transformation of HFS-induced LTP into LTD.Spanish Ministry of Economy and Competitivity MINECO-FEDER, Grant (BFU2014-56164-P and BFU2017-82494-P)Fundación Tatiana Pérez de Guzmán el BuenoUniversity of Castilla la Mancha5.372 JCR (2020) Q1, 78/295 Biochemistry & Molecular Biology1.75 SJR (2020) Q1, 69/438 BiochemistryNo data IDR 2020UE