A Secure Steganographic Algorithm Based on Frequency Domain for the Transmission of Hidden Information

This contribution proposes a novel steganographic method based on the compression standard according to the Joint Photographic Expert Group and an Entropy Thresholding technique. The steganographic algorithm uses one public key and one private key to generate a binary sequence of pseudorandom numbers that indicate where the elements of the binary sequence of a secret message will be inserted. The insertion takes eventually place at the first seven AC coefficients in the transformed DCT domain. Before the insertion of the message the image undergoes several transformations. After the insertion the inverse transformations are applied in reverse order to the original transformations. The insertion itself takes only place if an entropy threshold of the corresponding block is satisfied and if the pseudorandom number indicates to do so. The experimental work on the validation of the algorithm consists of the calculation of the peak signal-to-noise ratio (PSNR), the difference and correlation distortion metrics, the histogram analysis, and the relative entropy, comparing the same characteristics for the cover and stego image. The proposed algorithm improves the level of imperceptibility analyzed through the PSNR values. A steganalysis experiment shows that the proposed algorithm is highly resistant against the Chi-square attack

On analytic properties of Meixner-Sobolev orthogonal polynomials of higher order difference operators

In this contribution we consider sequences of monic polynomials orthogonal with respect to Sobolev-type inner product $$\left\langle f,g\right\rangle= \langle {\bf u}^{\tt M},fg\rangle+\lambda \mathscr T^j f (\alpha)\mathscr T^{j}g(\alpha),$$ where ${\bf u}^{\tt M}$ is the Meixner linear operator, $\lambda\in\mathbb{R}_{+}$, $j\in\mathbb{N}$, $\alpha \leq 0$, and $\mathscr T$ is the forward difference operator $\Delta$, or the backward difference operator $\nabla$. We derive an explicit representation for these polynomials. The ladder operators associated with these polynomials are obtained, and the linear difference equation of second order is also given. In addition, for these polynomials we derive a $(2j+3)$-term recurrence relation. Finally, we find the Mehler-Heine type formula for the $\alpha\le 0$ case

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

A Secure Steganographic Algorithm Based on Frequency Domain for the Transmission of Hidden Information

This contribution proposes a novel steganographic method based on the compression standard according to the Joint Photographic Expert Group and an Entropy Thresholding technique. The steganographic algorithm uses one public key and one private key to generate a binary sequence of pseudorandom numbers that indicate where the elements of the binary sequence of a secret message will be inserted. The insertion takes eventually place at the first seven AC coefficients in the transformed DCT domain. Before the insertion of the message the image undergoes several transformations. After the insertion the inverse transformations are applied in reverse order to the original transformations. The insertion itself takes only place if an entropy threshold of the corresponding block is satisfied and if the pseudorandom number indicates to do so. The experimental work on the validation of the algorithm consists of the calculation of the peak signal-to-noise ratio (PSNR), the difference and correlation distortionmetrics, the histogram analysis, and the relative entropy, comparing the same characteristics for the cover and stego image. The proposed algorithm improves the level of imperceptibility analyzed through the PSNR values. A steganalysis experiment shows that the proposed algorithmis highly resistant against the Chi-square attack