Platelet Rich Plasma stimulates human hair growth in vitro

Several factors are involved in hair growth and cycling (Buffoli et al., 2013). Platelet concentrates have a new important role in regenerative medicine and thus in dermatology, oral, plastic and orthopaedic surgery and hair growth (Franco et al., 2012). In this study we evaluated in vitro the effects of Platelet-Rich Plasma (PRP), an autologous platelet preparation, on hair growth. In particular, we compared four different culture media (Philpott et al., 1990): 1-William’s E culture medium with supplemented factors; 2-William’s E culture medium with supplemented factors and Platelet Rich Plasma; 3-William’s E culture medium without supplemented factors; 4-William’s E culture medium without supplemented factors but with PRP. Hair shaft elongation was measured at 0, 24, 48, 72 and 96 hours: digitally fixed images of slices were analyzed using an image analyzer considering as measurable portion the shaft part between the bulb upper border and the top of the hair end. The values obtained were used to calculate the percentage of elongation for each time. Growth in hair cultured with William’s E medium with supplemented factors and PRP resulted higher with respect to the other media. Moreover, these results suggest that PRP stimulates human hair growth in vitro

It is not invisible! A case report of 2 patients with scalp Lichen Planopilaris mimicking Androgenic Alopecia

Lichen Planopilaris is known as the form of Lichen Planus typical of the scalp. It is classified as a lymphatic disease and is characterized by chronic inflammation which leads to cicatricial alopecia. Its causes are not yet well characterized but its etiology seems to strongly correlates with infection, sensitization and pollution. A clear and objective diagnosis of Lichen Planopilaris is not simple but the evolution and strongly negative outcomes on scalp of people affected by, pose the need of an early diagnosis. In this work we report the case of a 27-year-old male and a 54-year-old female, respectively, in which a correct diagnosis of Lichen Planopilaris, followed the incorrect previous ones, was made by means of dermatoscopy and histopathological analysis, decisive tools for the diagnosis of this kind of pathology

Scalp bacterial shift in Alopecia areata.

The role of microbial dysbiosis in scalp disease has been recently hypothesized. However, little information is available with regards to the association between microbial population on the scalp and hair diseases related to hair growth. Here we investigated bacterial communities in healthy and Alopecia areata (AA) subjects. The analysis of bacterial distribution at the genus level highlighted an increase of Propionibacterium in AA subjects alongside a general decrease of Staphylococcus. Analysis of log Relative abundance of main bacterial species inhabiting the scalp showed a significant increase of Propionibacterium acnes in AA subjects compared to control ones. AA scalp condition is also associated with a significant decrease of Staphylococcus epidermidis relative abundance. No significant changes were found for Staphylococcus aureus. Therefore, data from sequencing profiling of the bacterial population strongly support a different microbial composition of the different area surrounded hair follicle from the epidermis to hypodermis, highlighting differences between normal and AA affected the scalp. Our results highlight, for the first time, the presence of a microbial shift on the scalp of patients suffering from AA and gives the basis for a larger and more complete study of microbial population involvement in hair disorders

Drug Treatment for Androgenetic Alopecia: First Italian Questionnaire Survey on what do Dermatologists Think about Finasteride

<p></p><p><b>Article full text</b></p> <p><br></p> <p>The full text of this article can be found here<b>.</b> <a href="https://link.springer.com/article/10.1007/s13555-018-0233-6">https://link.springer.com/article/10.1007/s13555-018-0233-6</a></p><p></p><p></p><p> </p><p><br></p> <p><b>Provide enhanced content for this article</b></p> <p><br></p> <p>If you are an author of this publication and would like to provide additional enhanced content for your article then please contact <a href="http://www.medengine.com/Redeem/”mailto:[email protected]”"><b>[email protected]</b></a>.</p> <p><br></p> <p>The journal offers a range of additional features designed to increase visibility and readership. All features will be thoroughly peer reviewed to ensure the content is of the highest scientific standard and all features are marked as ‘peer reviewed’ to ensure readers are aware that the content has been reviewed to the same level as the articles they are being presented alongside. Moreover, all sponsorship and disclosure information is included to provide complete transparency and adherence to good publication practices. This ensures that however the content is reached the reader has a full understanding of its origin. No fees are charged for hosting additional open access content.</p> <p><br></p> <p>Other enhanced features include, but are not limited to:</p> <p><br></p> <p>• Slide decks</p> <p>• Videos and animations</p> <p>• Audio abstracts</p> <p>• Audio slides</p><br><p></p

In vitro effects of Red LED Light on human epidermal keratinocytes: a skin regeneration strategy.

The Red LED (light-emitting diode) light is a promising approach for skin regeneration. Even if its mechanism of action is not clearly understood, its regenerative properties have been investigated since about ten years ago. Keratinocytes represent the first and primary target of this technology because of they are involved in epidermal reconstitution. In these experiments, a new treatment, named “Dermodinamica” (Elisor, Milan), with a wavelength of 630nm, has been used. Different factors produced by keratinocytes have a key role in skin regenerative process: transforming growth factor-b1 (TGF-b1) with a crucial role in maintaining skin homeostasis and in re-epithelialization but also the modulation of metalloproteinases (MMP), in particular 1 and 8, during extracellular matrix production. Moreover, ROS-detoxifying enzymes, such as superoxide dismutase 1 (SOD-1) and heme-oxygenase 1 (HO-1), have an important role in cutaneous wound repair. The aim of the present in vitro study was to evaluate the effect of Red LED light (Dermodinamica, Elisor, Milan) on normal human epidermal keratinocytes (NHEK) after 1 day and 3 days of exposition monitoring: cell viability using MTT assay; TGF-1 production using ELISA kit; expression of collagen type I, MMP-1, MMP-8, SOD-1 and HO-1, using immunohistochemical technique; morphological evaluation analyzing semi-fine sections. Moreover, short (15 min exposition) and prolonged (30 min exposition) treatments were investigated. The results showed an increase of cell viability with the short treatment (15 min of exposition) and a modulation of TGF-b1, ROS-detoxifying enzymes, metalloproteinases. Morphological data confirm the induction of cell cycle physiological progression of keratinocytes. These data indicate a positive role of Red LED light in promoting keratinocytes renewal

Intraepidermal Injections of Autologous Epidermal Cell Suspension: A new promising approach to Dermatological Disorders. Preliminary Study

Regenerative medicine is a modern approach of dermatological treatment, using Epidermal Cells of the interfollicular epidermis (ESCs) for their effect in skin regeneration in chronic ulcers and burns, melanoma, vitiligo, junctional epidermolysis bullosa. Intraepidermal injections of autologous epidermal cell suspension can be a new and very promising treatment for many other cutaneous disorders as non-scarring alopecia (Alopecia Areata, Androgenic Alopecia) or scarring alopecia (Lichern Plano Pilaris alopecia, Discoid Lupus Erithematosus alopecia), anti-aging therapies. The intraepidermal injection of an autologous epidermal cell suspension is a simple, fast and safe surgical procedure: a small, thin portion of the epidermis of the patient undergoes a treatment where a suspension with all the cells collected from the epidermis and cultured for 7 days is injected into the skin. Our preliminary study shows that a suspension contains a significant number of viable cells that survive at day 7 in culture. Our research is ongoing and is focusing on the typing of the different cells in the suspension and evaluation of the presence and the nature of stem cells