Image of histological analysis <i>(Movat pentachrome staining)</i> and correlation with SEM examination.

<p>Defect is filled with TBM, followed by IV injections. There is substantial bone formation, with numerous osteoid areas (black arrows) and rich bone marrow.</p

SEM backscattered electron images of samples explanted 8 weeks after irradiation from the intraosseous group (×20 magnification).

<p>Only the BCP-TBM mixture induces significant new bone formation. White arrow indicates new bone formation.</p

HLA class I typing of LCL.

<p>ND : Not determined. LCL and the HLA triggering allorecognition are indicated in bold.</p

Outline of the protocol.

<p>Events concerning all animals (<i>n</i> = 20): white; events for the IV group (<i>n</i> = 8): dotted; events for the intraosseous group (<i>n</i> = 12): dashed.</p

SEM backscattered electron image of irradiated bone (×60 magnification).

<p>There is destruction of the bone microarchitecture and presence of poly-lobed gaps, suggesting Dambrain periosteocytic lysis.</p

Box plots representing the percentage of newly formed bone according to the different combinations.

<p>The circles are outliers, and the asterisks are extreme outliers. The rate of bone ingrowth is significantly higher after lysate IV injection, particularly with TBM in the defect, with or without BCP (ns).</p

SEM backscattered electron images of samples from the IV group (×20).

<p>Defects filled with BCP-TBM and TBM only and followed by IV injections induced significant new bone formation. White arrows indicate new bone formation.</p

Image of histological analysis <i>(Movat pentachrome staining)</i> and correlation with SEM examination.

<p>Defect is filled with TBM, followed by IV injections. There is substantial bone formation, with numerous osteoid areas (black arrows) and rich bone marrow.</p

Allorecognition of human endothelial cell cultures by CD8 T cell lines enriched in herpesvirus-specific T cells.

<p>T cell lines from D01 and D03, sorted with pp65_495–503/A*0201 multimers, were tested against HAEC #4373, #3315, and #3376, while T cell line from D15, sorted with BZLF1_54–64/B*3501 multimers, was tested against HAEC #3643 (Cw*0602+) and #1415 (Cw*0602-) for TNF-α production (<b>A</b>) and cytotoxicity (Effector-Target ratio 15∶1) (<b>B</b>). TNF-α production was measured as indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012120#pone-0012120-g002" target="_blank">Figure 2</a> legend. Only the endothelial cell lines expressing the relevant allogeneic HLA allele induced cytotoxicity and TNF production by the CD8 T cell lines tested. The data are representative of 3 different experiments.</p

Analysis of the TCR Vb repertoire of pp65_495–502/A*0201- or BZLF1/B*3501-sorted T cell lines, using TCR Vβ-specific mAb.

<p>The percentage of pp65/A*0201- or BZLF1/B*3501-specific T cells stained by the various anti-TCR Vβ mAb is mentioned according to the IMGT nomenclature (Beckman Coulter anti-TCR Vβ name is indicated in bracket). All T cell lines were derived from PBL, either from healthy donors or from RA patients.</p>a<p>Percentage determined by TCR sequencing (ref. 28). T cell lines exhibiting alloreactivity are marked in bold.</p