Planococcus versutus sp. nov., isolated from soil

A taxonomic study was performed on a novel Gram-stain-positive, coccus-shaped, orange-pigmented motile bacterium, designated as strain L10.15T. The organism was isolated from a soil sample collected in Lagoon Island (close to Adelaide Island, western Antarctic Peninsula) using a quorum-quenching enrichment medium. Growth occurred at 4–30 °C, pH 6–11 and at moderately high salinity (0–15 %, w/v, NaCl), with optimal growth at 26 °C, at pH 7–8 and with 6 % (w/v) NaCl. 16S rRNA gene sequence analysis showed that strain L10.15T belonged to the genus Planococcus and was closely related to Planococcus halocryophilus Or1T (99.3 % similarity), Planococcus donghaensis JH1T (99.0 %), Planococcus antarcticus DSM 14505T (98.3 %), Planococcus plakortidis AS/ASP6 (II)T (97.6 %), Planococcus maritimus TF-9T (97.5 %), Planococcus salinarum ISL-6T (97.5 %) and Planococcus kocurii NCIMB 629T (97.5 %). However, the average nucleotide identity-MUMmer analysis showed low genomic relatedness values of 71.1–81.7 % to the type strains of these closely related species of the genus Planococcus . The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c and anteiso-C17 :  0, and the major menaquinones of strain L10.15T were MK-5 (48 %), MK-6 (6 %) and MK-7 (44 %). Polar lipid analysis revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophospholipid. The DNA G+C content was 39.4 mol%. The phenotypic and genotypic data indicate that strain L10.15T represents a novel species of the genus Planococcus , for which the name Planococcus versutus sp. nov. is proposed. The type strain is L10.15T (=DSM 101994T=KACC 18918T)

Acetobacter vaccinii sp. nov., a novel acetic acid bacterium isolated from blueberry fruit (Vaccinium corymbosum L.)

Strain C17-3T was isolated from blueberry fruits collected from a farmland located in Damyang-gun, Jeollanam-do, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences allocated strain C17-3T to the genus Acetobacter , where it occupied a rather isolated line of descent with Acetobacter ghanensis 430AT and Acetobacter lambici LMG 27439T as the nearest neighbours (98.9 % sequence similarity to both species). The highest average nucleotide identity and digital DNA–DNA hybridization values were 76.3 % and 21.7 % with Acetobacter garciniae TBRC 12339T; both values were well below the cutoff values for species delineation. Cells are strictly aerobic, Gram-stain-negative rods, catalase-positive and oxidase-negative. The DNA G+C content calculated from the genome sequence was 59.2 %. Major fatty acids were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c) and C19 : 0cyclo ω8c. The major isoprenoid quinone was ubiquinone 9. On the basis of the results of phylogenetic analyses, phenotypic features and genomic comparisons, it is proposed that strain C17-3T represents a novel species of the genus Acetobacter and the name Acetobacter vaccinii sp. nov. is proposed. The type strain is C17-3T (= KACC 21233T = LMG 31758T).</jats:p

Arachidicoccus soli Lee & Kim & Sang & Song & Kwon & Weon 2021, SP. NOV.

DESCRIPTION OF ARACHIDICOCCUS SOLISP. NOV. Arachidicoccus soli (so′ li. L. gen. n. soli of soil). Cells are aerobic, Gram-stain-negative, non-motile and rod-shaped (0.5–0.6 µm wide and 1.3–10 µm long). Colonies grown at 28 °C on R2A agar for 2 days are light yellow, round and convex. Growth occurs at 10–33 °C, pH 6.0–7.5 and with 0–4 % NaCl (w/v). Tests for catalase and oxidase are negative. Tyrosine is hydrolysed, but casein, CM-cellulose, hypozanthine, DNA and Tween 80 are not. Positive for aesculin hydrolysis and β -galactosidase, but negative for nitrate reduction, indole production, glucose fermentation, arginine dihydrolase, urease and gelatin hydrolysis. Assimilates D-glucose, L-arabinose, D-mannose, N -acetylglucosamine, maltose, L-rhamnose and L-proline, but does not assimilate D-mannitol, potassium gluconate, capric acid, adipic acid, malic acid, trisodium citrate, phenylacetic acid, D-ribose, inositol, sucrose, itaconic acid, suberic acid, sodium malonate, sodium acetate, lactic acid, L-alanine, potassium 5-ketogluconate, glycogen, 3-hydroxybenzoic acid, L-serine, salicin, melibiose, L-fucose,D-sorbitol, propionic acid, valeric acid, L-histidine, potassium 2-ketogluconate, 3-hydroxybutyric acid and 4-hydroxybenzoic acid. Positive activities for alkaline phosphatase, esterase (C4), leucine arylamidase, valine arylamidase, cystine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, α-galactosidase, β -glucuronidase, α-glucosidase, N - acetylβ -glucosaminidase and α-fucosidase, but negative activities for esterase lipase (C8), lipase (C14), trypsin, α-chymotrypsin, β -glucosidase and α-mannosidase. The major respiratory quinone is MK-7. Polar lipids consist of phosphatidylethanolamine, four unknown aminolipids and three unknown lipids. The major fatty acids are iso-C 15:0, iso-C 17:0 3-OH and iso-C 15:1 G. The major polyamines were homospermidine and putrescine. The genomic DNA G+C content of the type strain is 36.4mol%. Data was taken from a, Madhaiyan et al. [1], b, Siddiqi et al. [2], and c, Siddiqi et al. [3]. *Summed features are groups of two or three fatty acids that cannot be separated by the MIDI system. Summed feature 2 comprised C12:0 aldehyde and/or unknown equivalent chain length (ECL) 10.928 and/or C14:0 3-OH and/or iso-C16:1 I. Summed feature 3 comprised C16:1 ω 6 c and/or C16:1 ω 7 c. The type strain, KIS59-12 T (= KACC 17340 T = NBRC 113161 T), was isolated from a soil sample collected on Hodo island, Boryeong, Chungcheongnam-do Province, Republic of Korea. The GenBank / EMBL / DDBJ accession numbers for the 16S rRNA gene sequence and the genome sequence of strain KIS59-12 T are MH 933978 and CP032489, respectively.Published as part of Lee, Shin Ae, Kim, Tae-Wan, Sang, Mee-Kyung, Song, Jaekyeong, Kwon, Soon-Wo & Weon, Hang-Yeon, 2021, Arachidicoccus soli sp. nov., a bacterium isolated from soil, pp. 1-6 in International Journal of Systematic and Evolutionary Microbiology (004566) (004566) 71 (1) on pages 4-5, DOI: 10.1099/ijsem.0.004566, http://zenodo.org/record/604870

Streptomyces brevispora sp nov and Streptomyces laculatispora sp nov., actinomycetes isolated from soil

The taxonomic positions of two actinomycetes isolated from a hay meadow soil sample were determined using a polyphasic approach. The isolates had chemical and morphological properties typical of streptomycetes and formed a distinct 16S rRNA gene subclade together with the type strain Streptomyces drozdowiczii NRRL B-24297(T). DNA DNA relatedness studies showed that the three strains belonged to different genomic species. The organisms were also distinguished using a combination of phenotypic properties. On the basis of these data it is proposed that the isolates be assigned to the genus Streptomyces as Streptomyces brevispora sp. nov. and Streptomyces laculatispora sp. nov., with BK160(T) (=KACC 21093(T) =NCIMB 14702(T)) and BK166(T) (=KACC 20907(T) =NCIMB 14703(T)) as the respective type strains.Conselho Nacional de Desenvolvimento Cientifico e TecnologicoConselho Nacional de Desenvolvimento Cientifico e Tecnologico [201066/2009-2

Control of wilt and rot pathogens of tomato by antagonistic pink pigmented facultative methylotrophic Delftia lacustris and Bacillus spp.

The studies on the biocontrol potential of pink pigmented facultative methylotrophic (PPFM) bacteria other than the genus Methylobacterium are scarce. In the present study, we report three facultative methylotrophic isolates; PPO-1, PPT-1 and PPB-1, respectively identified as Delftia lacustris, Bacillus subtilis and Bacillus cereus by 16S rRNA gene sequence analysis. Hemolytic activity was tested to investigate the potential pathogenicity of isolates to plants and humans, the results indicates that the isolates PPO-1, PPT-1 and PPB-1 are not pathogenic strains. Under in vitro conditions, D. lacustris PPO-1, B. subtilis PPT-1 and B. cereus PPB-1 showed direct antagonistic effect by inhibiting the mycelial growth of fungal pathogens; Fusarium oxysporum f. sp. lycopersici (2.15, 2.05 and 1.95 cm), Sclerotium rolfsii (2.14, 2.04 and1.94 cm), Pythium ultimum (2.12, 2.02 and 1.92cm), and Rhizoctonia solani (2.18, 2.08 and 1.98 cm) and also produced volatile inhibitory compounds. Under plant growth chamber condition methylotrophic bacterial isolates; D. lacustris PPO-1, B. subtilis PPT-1 and B. cereus PPB-1 significantly reduced the disease incidence of tomato. Under greenhouse condition, D. lacustris PPO-1, B. subtilis PPT-1 and B. cereus PPB-1 inoculated tomato plants, when challenged with F. oxysporum f. sp. lycopersici, S. rolfsii, P. ultimum and R. solani, increased the pathogenesis related proteins (β-1, 3-glucanase and chitinase) and defense enzymes (phenylalanine ammonia lyase, peroxidase, polyphenol oxidase, and catalase) on day 5 after inoculation. In the current study, we first report the facultative methylotrophy in pink pigmented Delftia lacustris, B. subtilis, and B. cereus and their antagonistic potential against fungal pathogens. Direct antagonistic and ISR effects of these isolates against fungal pathogens of tomato evidenced their possible use as a biocontrol agent