Oral human papillomavirus (HPV) infection in men who have sex with men: prevalence and lack of anogenital concordance.

To estimate the prevalence of oral detectable human papillomavirus (HPV) DNA in HIV-negative men who have sex with men (MSM) attending a sexual health clinic in London and concordance with anogenital HPV infection. Such data are important to improve our understanding of the epidemiology of oral HPV and the potential use of vaccines to prevent oropharyngeal cancers

The rod domain is not essential for the function of plectin in maintaining tissue integrity

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.-- et al.Epidermolysis bullosa simplex associated with late-onset muscular dystrophy (EBS-MD) is an autosomal recessive disorder resulting from mutations in the plectin gene. The majority of these mutations occur within the large exon 31 encoding the central rod domain and leave the production of a low-level rodless plectin splice variant unaffected. To investigate the function of the rod domain, we generated rodless plectin mice through conditional deletion of exon 31. Rodless plectin mice develop normally without signs of skin blistering or muscular dystrophy. Plectin localization and hemidesmosome organization are unaffected in rodless plectin mice. However, superresolution microscopy revealed a closer juxtaposition of the C-terminus of plectin to the integrin β4 subunit in rodless plectin keratinocytes. Wound healing occurred slightly faster in rodless plectin mice than in wild-type mice, and keratinocytes migration was increased in the absence of the rod domain. The faster migration of rodless plectin keratinocytes is not due to altered biochemical properties because, like full-length plectin, rodless plectin is a dimeric protein. Our data demonstrate that rodless plectin can functionally compensate for the loss of full-length plectin in mice. Thus the low expression level of plectin rather than the absence of the rod domain dictates the development of EBS-MD.This work was supported financially by grants from DEBRA UK and the Netherlands Organization for Scientific Research (NWO/ALW). J.M.d.P. was supported by the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund (Grant BFU2012-32847).Peer Reviewe

Mener ou influencer?

One skill set identified within the CanMEDS Framework (CanMEDS) as essential to training future physicians is the Leader role.  Arguably however, the term Leader carries certain connotations that are inconsistent with the abilities outlined by CanMEDS as necessary for physicians.  For example, the term Leader may connote hierarchical authority and formalized responsibilities, while de-emphasizing informal day-to-day influencing.  This CanMEDS role was first labelled Manager, but was re-named Leader in 2015.  Perhaps the focus of this CanMEDS role should be further refined by adopting a more representative term that reflects the concept of intentional influence. Through this lens, learners can discern significant opportunities to influence positively each of the clinical and non-clinical environments they encounter.  We suggest that re-framing the Leader role as an Influencer role will be more comprehensive and inclusive of its full scope and potential.   Accordingly, given the potential for broader applicability and resonance with learners, collaborators, and the populations we serve, consideration should be given to re-characterizing the CanMEDS role of Leader as that of Influencer.Le rôle de Leader est une des compétences du Référentiel CanMEDS jugées essentielles dans la formation des futurs médecins. Cependant, on peut soutenir que la notion de leadership comporte certaines connotations qui sont incompatibles avec les compétences exigées dans CanMEDS. Par exemple, le terme « leader » peut évoquer une autorité hiérarchique et des responsabilités formelles, tout en minimisant l'influence informelle exercée au quotidien. Avant 2015, ce rôle était désigné par le mot « gestionnaire ». Peut-être l'orientation de ce rôle CanMEDS devrait-elle être redéfinie et une appellation correspondante choisie pour refléter la notion d'influence intentionnelle. Une telle reformulation inciterait les apprenants à cerner les occasions importantes d'influencer positivement les environnements cliniques et non-cliniques dans lesquels ils travaillent. Nous sommes d'avis qu'un recadrage du rôle de leader en influenceur engloberait toute la portée et tout le potentiel auxquels le rôle renvoie. Le rôle d'Influenceur promet une applicabilité et une résonance plus larges auprès des apprenants, des collaborateurs et des populations que nous servons, d’où la pertinence de la redéfinition du rôle CanMEDS actuel

The Signalling Effect of HRM on Psychological Contracts of Employees: A Multi-Level Perspective

Wat bepaalt de effectiviteit van HRM? Zowel in wetenschappelijk HRM onderzoek als in de HRM praktijk is de relatie tussen HRM en organisatie prestaties de afgelopen jaren uitgegroeid tot het belangrijkste aandachtsgebied. Centraal in deze relatie staat de verhouding tussen werknemers en de organisatie. Dit omdat HRM poogt bij te dragen aan organisatieprestatie door het optimaliseren van de verhouding tussen werknemers en de organisatie. Huidig onderzoek is niet in staat geweest deze relatie empirisch te onderbouwen. Deze studie concentreert zich daarom op het vullen van deze leemte door de relevantie van HRM voor de arbeidsrelatie empirisch te onderzoeken. Hierbij wordt een brug geslagen tussen twee stromen van HRM onderzoek: - op het niveau van de organisatie, onderzoek naar de relatie tussen HRM en organisatieprestatie; - op individueel niveau, onderzoek naar de relatie tussen werkbeleving en individuele prestaties. Het centrale onderwerp van deze studie is het psychologisch contract. Het psychologisch contract brengt de twee stromen onderzoek samen door haar focus op de uitwisselingsrelatie tussen organisatie en medewerker. Gebaseerd op een steekproef van 49 organisaties (verschillende industrieën en grootte) en 2099 medewerkers wordt het signaal effect van HRM voor de mate waarin medewerkers schending van hun psychologisch contract ervaren onderzocht. Hierbij worst gebruik gemaakt van een multi-level analyse techniek. De uitkomsten van dit onderzoek wijzen op een significant verband tussen HRM, organisatorische ontwerpfactoren en de mate waarin medewerkers schending van hun psychologisch contract ervaren. Daarmee geeft deze studie als één van de eerste empirisch inzicht in de wijze waarop HRM leidt tot effectiviteit.What are the drivers of high performing organizations? In HRM research and practice, the relationship between HRM and organizational performance dominates the research of last decade. Employees and their relationship with the organization take central stage in this perspective since HRM aims to contribute to organizational performance while optimizing the relationship between employees and organization. However, HRM research has failed to empirically establish this relationship. This study attempts address this research gap by empirically investigating the relevance of HRM for the employee organization relationship. In doing so, this study ‘bridges’ two streams of HRM research, organizational level research on HRM and performance and individual level theorizing on employee work perceptions and behavioral performance. We focus our attention on the concept of the psychological contract. The psychological contract connects organizational level and individual level perspectives because of its focus on the exchange relationship between organization and individual. Based on a sample of 49 organizations with 2099 individual respondents we have investigated, using a multi-level analytical technique, the signalling value of HRM on degree of psychological contract violation of employees. Our findings indicate that there is a significant relationship between HRM, organizational design factors and the degree of perceived psychological contract violation. Our findings provide us with one of the first empirical indications on how HRM leads to effectiveness

Automated SNP genotype clustering algorithm to improve data completeness in high-throughput SNP genotyping datasets from custom arrays

High-throughput SNP genotyping platforms use automated genotype calling algorithms to assign genotypes. While these algorithms work efficiently for individual platforms, they are not compatible with other platforms, and have individual biases that result in missed genotype calls. Here we present data on the use of a second complementary SNP genotype clustering algorithm. The algorithm was originally designed for individual fluorescent SNP genotyping assays, and has been optimized to permit the clustering of large datasets generated from custom-designed Affymetrix SNP panels. In an analysis of data from a 3K array genotyped on 1,560 samples, the additional analysis increased the overall number of genotypes by over 45,000, significantly improving the completeness of the experimental data. This analysis suggests that the use of multiple genotype calling algorithms may be advisable in high-throughput SNP genotyping experiments. The software is written in Perl and is available from the corresponding author

Forming new sex partnerships while overseas: findings from the third British national survey of sexual attitudes & lifestyles (Natsal-3)

Objectives: Travelling away from home presents opportunities for new sexual partnerships, which may be associated with sexually transmitted infection (STI) risk. We examined the prevalence of, and factors associated with, reporting new sexual partner(s) while overseas, and whether this differed by partners’ region of residence. Methods: We analysed data from 12 530 men and women aged 16–74 years reporting ≥1 sexual partner(s) in the past 5 years in Britain's third National Survey of Sexual Attitudes and Lifestyles (Natsal-3), a probability survey undertaken 2010–2012. Results: 9.2% (95% CI 8.3% to 10.1%) of men and 5.3% (4.8% to 5.8%) of women reported new sexual partner(s) while overseas in the past 5 years. This was strongly associated with higher partner numbers and other sexual and health risk behaviours. Among those with new partners while overseas, 72% of men and 58% of women reported partner(s) who were not UK residents. Compared with those having only UK partners while abroad, these people were more likely to identify as ‘White Other’ or ‘Non-White’ (vs White British ethnicity), report higher partner numbers, new partners from outside the UK while in the UK and paying for sex (men only) all in the past 5 years. There was no difference in reporting STI diagnosis/es during this time period. Conclusions: Reporting new partners while overseas was associated with a range of sexual risk behaviours. Advice on sexual health should be included as part of holistic health advice for all travellers, regardless of age, destination or reason for travel

Traffic-Light Labels and Choice Architecture: Promoting Healthy Food Choices

Background: Preventing obesity requires maintenance of healthy eating behaviors over time. Food labels and strategies that increase visibility and convenience of healthy foods (choice architecture) promote healthier choices, but long-term effectiveness is unknown. Purpose: Assess effectiveness of traffic-light labeling and choice architecture cafeteria intervention over 24 months. Design: Longitudinal pre–post cohort follow-up study between December 2009 and February 2012. Data were analyzed in 2012. Setting/participants: Large hospital cafeteria with a mean of 6511 transactions daily. Cafeteria sales were analyzed for (1) all cafeteria customers and (2) a longitudinal cohort of 2285 hospital employees who used the cafeteria regularly. Intervention: After a 3-month baseline period, cafeteria items were labeled green (healthy); yellow (less healthy); or red (unhealthy) and rearranged to make healthy items more accessible. Main outcome measures: Proportion of cafeteria sales that were green or red during each 3-month period from baseline to 24 months. Changes in 12- and 24-month sales were compared to baseline for all transactions and transactions by the employee cohort. Results: The proportion of sales of red items decreased from 24% at baseline to 20% at 24 months (p\u3c0.001), and green sales increased from 41% to 46% (p\u3c0.001). Red beverages decreased from 26% of beverage sales at baseline to 17% at 24 months (p\u3c0.001); green beverages increased from 52% to 60% (p\u3c0.001). Similar patterns were observed for the cohort of employees, with the largest change for red beverages (23%–14%, p\u3c0.001). Conclusions: A traffic-light and choice architecture cafeteria intervention resulted in sustained healthier choices over 2 years, suggesting that food environment interventions can promote long-term changes in population eating behaviors

Expression of Integrin α6β4 in Junctional Epidermolysis Bullosa

The integrin α6β4 is a member of the integrin family of adhesion receptors. The integrin α6β4 is preferentially expressed in stratified squamous epithelia, where it is localized in hemidesmosomes. A reduced number of rudimentary he- midesmosomes is often found in skin from patients with junctional epidermolysis bullosa (JEB). In this study we have investigated the expression of α6β4 in skin specimens of patients with junctional (one non-lethal, two lethal) and dystrophic (two) epidermolysis bullosa, using immunofluorescent (IF) staining with five different monoclonal antibodies against the α6 and β4 subunits. The intensity of IF staining of the integrin α6β4 and bullous pemphigoid antigen (BPA) was unreduced along the epidermal basement membrane zone (EBMZ) of all EB patients, compared to that in skin of healthy human controls. However, in the skin of two patients with lethal (Herlitz) JEB, who did not express GB3, IF staining of integrin α6β4 and BPA showed a “stitchy” discontinuous linear pattern along the EBMZ with interruptions at the borders of adjoining basal keratinocytes. The same results were obtained by immunoelectron microscopy. They corresponded with freeze-induced partial cell detachment from the basement membrane at the ultimate baso-lateral edge ot the basal keratinocytes in lethal JEB skin. The basal lamellipodia at that location almost completely lacked tonofilaments and hemidesmosomes. Furthermore, in JEB there was a split between the intra- and extracellular epitopes of the integrin α6β4 receptor, whereas the integrin remains intact in salt-split skin. This suggests that the defect is in α6β4 itself or perhaps its ligand

Mechanistic insight in the selective delignification of wheat straw by three white-rot fungal species through quantitative 13C-IS py-GC–MS and whole cell wall HSQC NMR

Background The white-rot fungi Ceriporiopsis subvermispora (Cs), Pleurotus eryngii (Pe), and Lentinula edodes (Le) have been shown to be high-potential species for selective delignification of plant biomass. This delignification improves polysaccharide degradability, which currently limits the efficient lignocellulose conversion into biochemicals, biofuels, and animal feed. Since selectivity and time efficiency of fungal delignification still need optimization, detailed understanding of the underlying mechanisms at molecular level is required. The recently developed methodologies for lignin quantification and characterization now allow for the in-depth mapping of fungal modification and degradation of lignin and, thereby, enable resolving underlying mechanisms. Results Wheat straw treated by two strains of Cs (Cs1 and Cs12), Pe (Pe3 and Pe6) and Le (Le8 and Le10) was characterized using semi-quantitative py-GC–MS during fungal growth (1, 3, and 7 weeks). The remaining lignin after 7 weeks was quantified and characterized using ¹³C lignin internal standard based py-GC–MS and whole cell wall HSQC NMR. Strains of the same species showed similar patterns of lignin removal and degradation. Cs and Le outperformed Pe in terms of extent and selectivity of delignification (Cs ≥ Le >> Pe). The highest lignin removal [66% (w/w); Cs1] was obtained after 7 weeks, without extensive carbohydrate degradation (factor 3 increased carbohydrate-to-lignin ratio). Furthermore, though after treatment with Cs and Le comparable amounts of lignin remained, the structure of the residual lignin vastly differed. For example, Cα-oxidized substructures accumulated in Cs treated lignin up to 24% of the total aromatic lignin, a factor two higher than in Le-treated lignin. Contrarily, ferulic acid substructures were preferentially targeted by Le (and Pe). Interestingly, Pe-spent lignin was specifically depleted of tricin (40% reduction). The overall subunit composition (H:G:S) was not affected by fungal treatment. Conclusions Cs and Le are both able to effectively and selectively delignify wheat straw, though the underlying mechanisms are fundamentally different. We are the first to identify that Cs degrades the major β-O-4 ether linkage in grass lignin mainly via Cβ–O–aryl cleavage, while Cα–Cβ cleavage of inter-unit linkages predominated for Le. Our research provides a new insight on how fungi degrade lignin, which contributes to further optimizing the biological upgrading of lignocellulose. Electronic supplementary material The online version of this article (10.1186/s13068-018-1259-9) contains supplementary material, which is available to authorized users