Synthesis of Polyurethanes based on 17 Hydroxy Oleic Acid obtained from Sophorolipids

Due to the worldwide shortage of petrochemical based resources, the usage of renewable bio-based raw materials for established and novel products becomes increasingly important.[1] Such bio-based resources are already used for the fabrication of a variety of products, e. g. paper, lubricants, detergents or cosmetics. In the future they are expected to emerge in many more applications in industry and household.[1] A very promising approach relies on the use of glycolipids as a source of hydroxy-oleic acid.[2] Microbial glycolipids are produced for instance via fermentation from natural resources such as plant oils and sugar.[3] After fermentation complex product mixtures are obtained with the composition depending on the microorganism, substrate and fermentation time.[3] The successful use of microbial glycolipids and hydroxy-oleic acid (HOA) derived therefrom as bio-based intermediates requires reliable analytical methods as well as robust manufacturing processes for the synthesis and cleavage of bio-based molecules. In order to obtain hydroxy-oleic acids as bio-based intermediates, the acidic cleavage of microbial derived sophorolipid was investigated. In addition the implementation of HOA in polyurethane (PU) systems was explored

Lipase catalyzed synthesis of oligomeric diol building blocks utilizing sophorolipid-derived hydroxy fatty acids

The synthesis of 17-hydroxy-oleic acid based oligomeric esters was investigated with immobilized Pseudozyma antarctica Lipase B and hexanediol as co-substrate. The effects of different reaction parameters on velocity and product composition at equilibrium conditions were analyzed. The synthesis of oleic acid esters was used as a reference system for initial evaluation of reaction parameters. The reaction with oleic acid and hexanediol was fastest at an enzyme concentration of 5% at 60 °C and high conversions of > 90 % were achieved in non-polar solvents in the presence of molecular sieves. In heptane an oleic acid conversion of 96 % was reached with a final diester to monoester ratio of > 4:1. In syntheses trials with 17-hydroxy-oleic acid the formation of oligomers was verified with GPC, however; conversion was generally lower than with oleic acid. Removal of hydroxyl fatty acid monomers and dimers and the formation ester functionalities could be verified by GC analysis. An increase of the degree of oligomerization was observed simultaneously by GPC analysis. The number-average molecular weight was around 1400 in the best trials corresponding to a degree of oligomerization of around 4 units of hydroxyl-fatty acid attached to a hexanediol core. Though transformations were not complete, the final oligomer size was in the lower range of polyester diols used for polyurethane manufacturing

Neural network regression for particle identification with the ALICE TPC detector in Run 3

The gaseous Time Projection Chamber (TPC) of the ALICE experiment at CERN serves some of the most crucial roles in many physics analyses within the collaboration and is responsible for 92.5% of the raw data taken with the experiment. One of its major advantages is extensive particle identification over a wide range of momenta. The basic underlying physics concerns the process of ionization of gas molecules and the associated specific energy loss of traversing particles, described by the Bethe-Bloch formula. In this thesis, a novel method for the tuning of parameters of the ALEPH parameterization of the Bethe-Bloch formula is presented based on the concept of hyperparameter optimization. A novel framework called OPTUNA and custom designed loss functions are investigated and tested against the performance on datasets with known particle identity from Run 2 of the LHC. Besides the parameterization, further corrections to the mean as well as an estimation of the standard deviation of particle data distributions have to be made in high dimensions, which forms the main body of this thesis. Both parts are approximated with fully connected feed-forward neural networks trained on identified daughter particles from weak decays of K^0_S , Λ, Λ ̄ and γ conversions. An average accuracy of around 3‰ for the mean correction based on a neural network ensemble is achieved. This is compared with the results obtained from one-dimensional spline corrections in Run 2 and it is shown that the neural network introduced in this thesis can perform similarly well as the approaches from Run 2 but shows significant improvements in higher dimensions since it does not rely on a factorization approach. The estimation of uncertainty of the distribution for each particle species is performed likewise, and an overall similar performance as the functional parameterization from Run 2 is achieved. However, due to the multidimensional mean corrections by the neural network and the limitations that a parameterized functional shape inherits, the standard deviation is captured better for all particle species by the neural network. In contrast to the Run 2 approach, this method works without additional iterations and consumes overall less time and effort for quality checks

Strategic investment decisions in multi-stage contests with heterogeneous players

Deutscher C, Sahm M, Schneemann S, Sonnabend H. Strategic investment decisions in multi-stage contests with heterogeneous players. Theory and Decision. 2021.When heterogeneous players make strategic investment decisions in multi-stage contests, they might conserve resources in a current contest to spend more in a subsequent contest, if the degree of heterogeneity in the current (subsequent) contest is sufficiently large (small). We confirm these predictions using data from German professional soccer, in which players are subject to a one-match ban if they accumulate five yellow cards. Players with four yellow cards facing the risk of being suspended for the next match are (i) less likely to be fielded when the heterogeneity in the current match increases and (ii) more likely to receive a fifth yellow card in the current match when heterogeneity in the next match increases or heterogeneity in the next match but one (when they return from their ban) decreases

Complex shoulder disorders: evaluation and treatment

Evaluation of patients with shoulder disorders often presents challenges. Among the most troublesome are revision surgery in patients with massive rotator cuff tear, atraumatic shoulder instability, revision arthroscopic stabilization surgery, adhesive capsulitis, and bicipital and subscapularis injuries. Determining functional status is critical before considering surgical options in the patient with massive rotator cuff tear. When nonsurgical treatment of atraumatic shoulder stability is not effective, inferior capsular shift is the treatment of choice. Arthroscopic revision of failed arthroscopic shoulder stabilization procedures may be undertaken when bone and tissue quality are good. Arthroscopic release is indicated when idiopathic adhesive capsulitis does not respond to nonsurgical treatment; however, results of both nonsurgical and surgical treatment of posttraumatic and postoperative adhesive capsulitis are often disappointing. Patients not motivated to perform the necessary postoperative therapy following subscapularis repair are best treated with arthroscopic débridement and biceps tenotomy

Influence of Using Perforated Plastic Flooring Beneath the Waterline on Growth Performance, Litter Quality, and Footpad Health of Broiler Chickens: A Field Study

The aim of this study was to evaluate the effect of slatted flooring beneath waterlines in broiler barns on litter quality and, subsequently, footpad health. The hypothesis tested was that installing slatted flooring underneath waterlines helps to improve litter quality and thus reduces footpad diseases, enhancing animal welfare as a result. Five experimental runs with two groups were conducted. Each run was defined as one fattening period of 32 days and consisted of 15,000 broiler Ross 308 of both sexes. Every barn was divided into three areas (drinkers, feeders, and comfort area) for weekly sampling. No influence on growth performance was noted. The slatted flooring influenced the litter quality by preventing the litter in the experimental group (EG = 690 ± 167 g/kg DM) from becoming moisture until day 14 of the fattening period compared to the control group (CON = 636 ± 198 g/kg DM). The footpad health was also influenced by using slatted flooring, with lower camera-based footpad scores in the EG (8.80) compared to CON (22.0) at the slaughterhouse (p = 0.0258). Installing slatted flooring beneath the waterline reduced the moisture of the litter compared to the control barn in the first two weeks of age and showed a positive effect on the footpad health of the broilers at the end of fattening, which indicates an improvement in animal welfare

The debate on HIV in Africa (multiple letters)

SCOPUS: le.jinfo:eu-repo/semantics/publishe

Non-canonical two-step biosynthesis of anti-oomycete indole alkaloids in Kickxellales

Abstract Background Fungi are prolific producers of bioactive small molecules of pharmaceutical or agricultural interest. The secondary metabolism of higher fungi (Dikarya) has been well-investigated which led to > 39,000 described compounds. However, natural product researchers scarcely drew attention to early-diverging fungi (Mucoro- and Zoopagomycota) as they are considered to rarely produce secondary metabolites. Indeed, only 15 compounds have as yet been isolated from the entire phylum of the Zoopagomycota. Results Here, we showcase eight species of the order Kickxellales (phylum Zoopagomycota) as potent producers of the indole-3-acetic acid (IAA)-derived compounds lindolins A and B. The compounds are produced both under laboratory conditions and in the natural soil habitat suggesting a specialized ecological function. Indeed, lindolin A is a selective agent against plant-pathogenic oomycetes such as Phytophthora sp. Lindolin biosynthesis was reconstituted in vitro and relies on the activity of two enzymes of dissimilar evolutionary origin: Whilst the IAA–CoA ligase LinA has evolved from fungal 4-coumaryl-CoA synthetases, the subsequently acting IAA-CoA:anthranilate N-indole-3-acetyltransferase LinB is a unique enzyme across all kingdoms of life. Conclusions This is the first report on bioactive secondary metabolites in the subphylum Kickxellomycotina and the first evidence for a non-clustered, two-step biosynthetic route of secondary metabolites in early-diverging fungi. Thus, the generally accepted “gene cluster hypothesis” for natural products needs to be reconsidered for early diverging fungi

Osseous Union after Mandible Reconstruction with Fibula Free Flap Using Manually Bent Plates vs. Patient-Specific Implants: A Retrospective Analysis of 89 Patients

The aim of this monocentric, retrospective clinical study was to evaluate the status of osseous union in uni- and poly-segmental mandible reconstructions regarding conventional angle-stable manually bent osteosynthesis plates (Unilock 2.0 mm) versus titan laser-melted PSI patient-specific implant’s (PSI). The clinical impact of PSI’s high stiffness fixation methods on bone healing and regeneration is still not well addressed. The special interest was in evaluating the ossification of junctions between mandible and fibula and between osteotomized fibula free flap (FFF) segments. Panoramic radiograph (OPT), computed tomography (CT) scans, or cone-beam CTs (CBCT) of patients who underwent successful FFF for mandible reconstruction from January 2005 to December 2020 were analyzed. A total number of 89 cases (28 females (31.5%), 61 males (68.5%), mean age 58.2 ± 11.3 years, range: 22.8–82.7 years) fulfilled the chosen inclusion criteria for analysis (conventional: n = 44 vs. PSI: n = 45). The present study found an overall incomplete ossification (IOU) rate of 24.7% (conventional: 13.6% vs. PSI: 35.6%; p = 0.017) for mandible to fibula and intersegmental junctions. Between osteotomized FFF segments, an IOU rate of 16% was found in the PSI-group, while no IOU was recorded in the conventional group (p = 0.015). Significant differences were registered for IOU rates in poly-segmental (p = 0.041), and lateral (p = 0.016) mandibular reconstructions when PSI was used. Multivariate logistic regression analysis identified plate exposure and type of plate used as independent risk factors for IOU. Previous or adjuvant radiotherapy did not impact incomplete osseous union in the evaluated study sample. PSI is more rigid than bent mini-plates and shields functional mechanical stimuli, and is the main reason for increasing the rate of incomplete ossification. To enhance the functional stimulus for ossification it has to be discussed if patient-specific implants can be designed to be thinner, and should be divided into segmental plates. This directs chewing forces through the bone and improves physiological bone remodeling

Host genetic loci LZTFL1 and CCL2 associated with SARS-CoV-2 infection and severity of COVID-19

OBJECTIVES: Host genetic factors contribute to the variable severity of COVID-19. We examined genetic variants from genome-wide association studies and candidate gene association studies in a cohort of patients with COVID-19 and investigated the role of early SARS-CoV-2 strains in COVID-19 severity. METHODS: This case-control study included 123 COVID-19 cases (hospitalized or ambulatory) and healthy controls from the state of Baden-Wuerttemberg, Germany. We genotyped 30 single nucleotide polymorphisms, using a custom-designed panel. Cases were also compared with the 1000 genomes project. Polygenic risk scores were constructed. SARS-CoV-2 genomes from 26 patients with COVID-19 were sequenced and compared between ambulatory and hospitalized cases, and phylogeny was reconstructed. RESULTS: Eight variants reached nominal significance and two were significantly associated with at least one of the phenotypes “susceptibility to infection”, “hospitalization”, or “severity”: rs73064425 in LZTFL1 (hospitalization and severity, P <0.001) and rs1024611 near CCL2 (susceptibility, including 1000 genomes project, P = 0.001). The polygenic risk score could predict hospitalization. Most (23/26, 89%) of the SARS-CoV-2 genomes were classified as B.1 lineage. No associations of SARS-CoV-2 mutations or lineages with severity were observed. CONCLUSION: These host genetic markers provide insights into pathogenesis and enable risk classification. Variants which reached nominal significance should be included in larger studies