RAZVOJ HOMOZIGOTNIH LINIJA KUKURUZA POMOĆU INDUKCIJE HAPLOIDA IN VIVO U HRVATSKOJ GERMPLAZMI

The in vivo haploid induction has been widely applied to the maize breeding in recent decades, but it has not been used in the breeding programs in the Republic of Croatia by now. This study\u27s objectives were to examine the haploid induction rates in the Croatian germplasm and to evaluate the properties of the D0 haploids, which are essential for a successful implementation of this method in breeding. The in vivo haploid induction was performed on 11 single-cross hybrids using the Zarodyshevy Marker Krasnodarsky (ZMK) inducer, and colchicine was used as a chromosome doubling agent. Emergence, misclassification rate, colchicine treatment survival, chromosome doubling rate and self-pollination success were examined in the D0 generation. The haploid induction rates ranged from 6.9 to 15.8%, which is consistent with the average induction rates characteristic of the ZMK inducer and the other modern ones. Significant differences were found among the populations of D0 haploids for all tested properties, except for self-pollination success. On average, the misclassification rates were lower, and the seedling survival rates were higher than those reported in other studies, indicating a possibility of a successful application of the doubled haploid method in maize breeding.Indukcija haploida in vivo posljednjih se desetljeća sve više primjenjuje u oplemenjivanju kukuruza, ali se dosad nije koristila u oplemenjivačkim programima u Republici Hrvatskoj. Ciljevi ovoga istraživanja bili su utvrditi relativne frekvencije induciranih haploida kod germplazme koja se upotrebljava u domaćim oplemenjivačkim programima i ispitati svojstva haploida generacije D0 koja su važna za uspješnu primjenu ove metode u oplemenjivanju. Indukcija haploida in vivo obavljena je na 11 jednostrukih hibrida pomoću induktora ZMK, a za udvostručenje kromosoma korišten je kolhicin. U generaciji D0 ispitana su svojstva nicanja, pogrešne klasifikacije, preživljavanja tretmana kolhicinom, udvostručenja kromosoma i uspješnosti izvođenja samooplodnje. Relativna frekvencija haploida kretala se od 6,9 do 15,8%, što je usporedivo s prosječnim stopama indukcije specifičnim za induktor ZMK i druge moderne induktore. Statistički značajne razlike pronađene su između populacija haploida D0 za sva ispitana svojstva, osim za uspješnost izvođenja samooplodnje. U prosjeku, stope pogrešne klasifikacije bile su niže, a stope preživljavanja tretmana kolhicinom više u odnosu na one zabilježene u drugim istraživanjima, što ukazuje na mogućnost uspješne primjene metode udvostručenih haploida u oplemenjivanju

Cellular expression and crystal structure of the murine cytomegalovirus MHC-Iv glycoprotein, m153

Mouse cytomegalovirus (MCMV), a β-herpesvirus that establishes latent and persistent infections in mice, is a valuable model for studying complex virus-host interactions. MCMV encodes the m145 family of putative immunoevasins with predicted MHC-I structure. Functions attributed to some family members include downregulation of host MHC-I (m152) and NKG2D ligands (m145, m152, m155) and interaction with inhibitory or activating NK receptors (m157). We present the cellular, biochemical and structural characterization of m153, which is a heavily glycosylated homodimer, that does not require β2m or peptide, and is expressed at the surface of MCMV-infected cells. Its 2.4 Å crystal structure confirms that this compact molecule preserves an MHC-I-like fold and reveals a novel mode of dimerization, confirmed by site-directed mutagenesis, and a distinctive disulfide-stabilized extended amino terminus. The structure provides a useful framework for comparative analysis of the divergent members of the m145 family

Tragači and Knjiški frikovi blogs – library services for children and young adults on the Web 2.0

Informatičko-tehnološki napredak osuvremenjuje knjižnične usluge, što omogućuje kontakt s korisnicima na daljinu. Prednosti novih tehnologija osobito su primjenjive u radu s mladim korisnicima koji se svakodnevno služe internetom. Pojavom novih internetskih aplikacija čitateljski klub, jedan od oblika rada s djecom i mladima s ciljem poticanja čitanja, koristi se njihovim mogućnostima te čini iskorak u virtualni prostor. Koristeći se pomagalima weba 2.0 (kao što su blog, Facebook, Google Docs), narodne knjižnice Bjelovara, Rijeke, Vinkovaca i Zadra pokreću blogove za djecu i mlade koji postaju online čitateljski klubovi uvijek otvoreni korisnicima svih knjižnica hrvatskoga govornog područja te rado posjećeno mjesto mladih čitatelja.Information and technological revolution modernizes library services and enables remote communication with patrons. The advantages of new technologies are particularly applicable in working with young patrons who use the Internet daily. Being one of the well known forms of encouraging reading among children and teenagers, book clubs now also use the possibilities of new Internet applications in order to step out into the virtual space. By applying the Web 2.0 tools (blog, Facebook, Google Docs) public libraries from Bjelovar, Rijeka, Vinkovci and Zadar managed to set up blogs for children and young adults functioning as online book clubs always open to patrons of all Croatian libraries and becoming a popular website for young readers

Tumor Necrosis Factor-Alpha and Interleukin 6 in Human Periapical Lesions

Aim. The aim of this study was to evaluate the presence of the cytokines tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in human periapical lesions. Subjects and methods. Samples were obtained from three groups of teeth: symptomatic teeth, asymptomatic lesions, and uninflamed periradicular tissues as a control. Results. TNF-alpha levels were significantly increased in symptomatic lesions compared to control. Group with asymptomatic lesions had significantly higher concentrations compared to control. There were no significant differences in TNF-alpha levels between symptomatic and asymptomatic lesions. In group with symptomatic lesions, IL-6 levels were significantly higher than in group with asymptomatic lesions. The IL-6 levels in symptomatic group also showed significantly higher concentration in comparison with control group. In asymptomatic group, the IL-6 level had significantly higher concentrations compared to control. Conclusion. These results indicate that symptomatic lesions represent an immunologically active stage of disease, and asymptomatic lesions are the point from which the process advances toward healing

Cellular Expression and Crystal Structure of the Murine Cytomegalovirus Major Histocompatibility Complex Class I-like Glycoprotein, m153

Mouse cytomegalovirus (MCMV), a β-herpesvirus that establishes latent and persistent infections in mice, is a valuable model for studying complex virus-host interactions. MCMV encodes the m145 family of putative immunoevasins with predicted MHC-I structure. Functions attributed to some family members include downregulation of host MHC-I (m152) and NKG2D ligands (m145, m152, m155) and interaction with inhibitory or activating NK receptors (m157). We present the cellular, biochemical and structural characterization of m153, which is a heavily glycosylated homodimer, that does not require β2m or peptide, and is expressed at the surface of MCMV-infected cells. Its 2.4 Å crystal structure confirms that this compact molecule preserves an MHC-I-like fold and reveals a novel mode of dimerization, confirmed by site-directed mutagenesis, and a distinctive disulfide-stabilized extended amino terminus. The structure provides a useful framework for comparative analysis of the divergent members of the m145 family

Brašno - Kruh '15

Proceedings contains 28 original research articles presented at 8th International Congress Flour – Bread ’15 and 10th Croatian Congress of Cereal Technologists Brašno – Kruh ’1

The influence of weather conditions on the agronomic traits of the os silage maize hybrids

Vremenske prilike tijekom vegetacije, a posebno sve učestalija pojava ekstrema poput suše, visokih temperatura ili nepravilne raspodjele oborina tijekom vegetacije značajno utječu na agronomska svojstva hibrida kukuruza, prije svega na urod zrna i prinos biljne mase/silaže. Stoga su potrebna kontinuirana istraživanja odnosa vremenskih prilika i hibrida sa svrhom utvrđivanja stabilnosti hibrida u različitim uvjetima vremenskih prilika proizvodnih godina. Cilj ovog istraživanja bio je analizirati utjecaj vremenskih prilika dvije vegetacijske godine (2017. i 2018.) na najznačajnija agronomska svojstva OS silažnih hibrida kukuruza. Pokus je postavljen na dvije lokacije (Popovac i Budrovci) po slučajnom bloknom rasporedu u tri ponavljanja. Istraživana su četiri genotipa kukuruza FAO grupe 550-650 (Velimir, OSSK 596, OSSK 617 i OSSK 635). Izvori variranja vremenskih prilika i genotipa imali su značajan učinak na istraživana agronomska svojstva. Koeficijenti varijabilnosti svojstava bili su različiti. Prosječne vrijednosti OS silažnih hibrida kukuruza kretale su se od 235 do 340 cm za visinu biljke do metlice, od 87 do 152 cm za visinu biljke do klipa, od 14 do 20 za broj redova zrna na klipu, od 38 do 56 za broj zrna u redu, od 15,2 t/ha do 21,7 t/ ha za prinos zrna s 35% vlage u zrnu te od 40,82 do 92,4 t/ha za prinos biljne mase.Weather conditions during vegetation, particularly more often occurrence of weather extremes such as dryness, high temperatures or irregular distribution of precipitation during vegetation significantly influence on agronomic traits of maize hybrids, primarily on yield of grain and biomass/silage. Therefore, continuous investigations of relationships between weather conditions and hybrids are needed in order to estimate the stability of hybrids in different conditions of weathers during the years of production. The aim of this investigation was to analyse the influence of weather conditions during two vegetation years (2017 and 2018) on most important agronomic traits of OS silage maize hybrids. Trail was done at two locations (Popovac and Budrovci) as randomized block with three replications. Four maize genotypes belonging to FAO maturity group 550-650 (Velimir, OSSK 596, OSSK 617 and OSSK 635) were investigated. Weather conditions and genotype as a source of variation had significant effect on investigated agronomic traits. Coefficients of variability of traits were different. Average values of OS silage maize hybrids varied from 235 to 340 cm for plant height, from 87 to 152 cm for ear height, from 14 to 20 rows of kernel on ear, from 38 to 56 of kernel per row, from 15.2 to 21.7 t/ha for grain yield with 35% of grain moisture content and from 40.82 to 92.4 t/ha for biomass yield

Tragači and Knjiški frikovi blogs – library services for children and young adults on the Web 2.0

Informatičko-tehnološki napredak osuvremenjuje knjižnične usluge, što omogućuje kontakt s korisnicima na daljinu. Prednosti novih tehnologija osobito su primjenjive u radu s mladim korisnicima koji se svakodnevno služe internetom. Pojavom novih internetskih aplikacija čitateljski klub, jedan od oblika rada s djecom i mladima s ciljem poticanja čitanja, koristi se njihovim mogućnostima te čini iskorak u virtualni prostor. Koristeći se pomagalima weba 2.0 (kao što su blog, Facebook, Google Docs), narodne knjižnice Bjelovara, Rijeke, Vinkovaca i Zadra pokreću blogove za djecu i mlade koji postaju online čitateljski klubovi uvijek otvoreni korisnicima svih knjižnica hrvatskoga govornog područja te rado posjećeno mjesto mladih čitatelja.Information and technological revolution modernizes library services and enables remote communication with patrons. The advantages of new technologies are particularly applicable in working with young patrons who use the Internet daily. Being one of the well known forms of encouraging reading among children and teenagers, book clubs now also use the possibilities of new Internet applications in order to step out into the virtual space. By applying the Web 2.0 tools (blog, Facebook, Google Docs) public libraries from Bjelovar, Rijeka, Vinkovci and Zadar managed to set up blogs for children and young adults functioning as online book clubs always open to patrons of all Croatian libraries and becoming a popular website for young readers

Corrosion Behavior of Different Types of Stainless Steel in PBS Solution

Anodic and spontaneous corrosion of different types of stainless steel (AISI 304L, AISI 316L and 2205 DSS) in phosphate-buffered saline solution (PBS, pH = 7.4) at 37 °C (i.e., in simulated physiological solution in the human body) were examined using open circuit potential measurements, linear and cyclic polarization, and electrochemical impedance spectroscopy methods. After the anodic and spontaneous corrosion, the surface of the tested samples was investigated by light and scanning electron microscopy (SEM) with EDS analysis. It has been established that the tendency of the examined steel materials towards local corrosion decreases in the order: AISI 304L < AISI 316L < 2205 DSS. Namely, the possibility of repassivation and the resistance to local corrosion increases in the same order. The corrosion resistance of steel samples at open circuit potential is a consequence of forming a natural oxide film with a bi-layer structure and consists of an inner barrier and an outer porous film. The inner barrier film has a small thickness and extremely high resistance, while the outer porous film is much thicker but also has significantly lower resistance. The inner barrier layer mainly prevents corrosion of examined steel samples in order: AISI 304L < AISI 316L < 2205 DSS. Light microscopy and SEM/EDS analysis after pitting and spontaneous corrosion showed damage on the AISI 304L and AISI 316L surface, while the surface of 2205 DSS was almost undamaged by corrosion

Variability and relationships of important alfalfa germplasm agronomic traits

Background and Purpose: The objective of this paper was to establish variability as well as to estimate stability of the most important alfalfa agronomic traits, with respect to germplasms of different origin. Correlations between the investigated traits were also analysed, since data on relationships are of great importance in selection, especially in traits with low genetic variability. Material and Methods: Experimental material consisted of 12 alfalfa germplasms. Investigation was carried out during a two-year period on two locations, at two different soil types. The field trial was arranged according to the randomised block design with four replications. Results and Conclusions: There was a statistically significant difference of green mass yield from 59.43 t ha–1 (Astra) to 69.04 t ha–1 (PCP), of dry matter from 12.26 t ha–1 (Astra) to 14.10 t ha–1 (PCP), of proteins from 2.49 t ha–1 (Panonija) to 3.03 t ha–1 (PCP), of plant height from 54.67 cm (OS – 90) to 60.82 (Elena), of leaf ratio to green mass from 51.03% (Elena) to 53.56% (Panonija) and of protein content in dry matter from 19.86% (Posavina) to 20.96% (PCP). There were positive correlations between green mass yield, dry matter, protein level and plant height, leaf ratio in green mass was in a negative correlation to yields and plant height. High and stable green mass yields were provided by germplasm OS-88, Du Puits, Elena, Posavina, Vuka and the breeding population PCP in various environmental conditions of plant growth