Phytochemical and antimicrobial studies of four species of Cola Schott & Endl. (Sterculiaceae)

The in-vitro antimicrobial evaluation of ethanol extracts of four species of Cola Schott & Endl. was done using human isolated strains of Staphylococcus aureus, Staphylococcus albus, Bacillus subtilis, Klebsiella pneumonia, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger as test organisms. The assays were carried out by agar well diffusion, erythromycin and ketoconazole served as the control drugs. The leaf ethanol extracts of the plants were found to be more effective against the tested fungi than the bacteria at high concentrations. None of the extracts was active against Staphylococcus aureus. Plant extract of C. acuminata (P.Beauv.) Schott & Endl. and C. nitida (Vent) Schott & Endl. showed activity on S. albus at concentrations ranging from 10-150 mgmlˉ¹ having comparable diameters of zone of inhibition of 7.3±0.03-16.0±0.0 for C. acuminata and 10.0±0.0-19.0±0.0 for C. nitida. Also, these two species of Cola demonstrated activities on C. albicans and A. niger at concentrations ranging from 90-150mgmlˉ¹ with relatively close diameters of zone of inhibition. Only C. acuminata inhibited the growth of K. pneumoniae at the MIC of 90mgmlˉ¹ whereas, C. albicans was inhibited by C. acuminata, C. millenii K. Schum and C. gigantea A.Chev. at the MIC of 120mgmlˉ¹. Phytochemical screening of the four species of Cola showed the presence of alkaloids, saponins, tannins and cardenolides in all the plants which apart from showing the probable closeness of the species could also be responsible for the observed activities. The antimicrobial property shown by the plant extracts is an evidence of the ethnomedicinal uses of the plants. The similarity observed in the phytochemical constituents and antimicrobial activities demonstrated by C nitida (Vent.) Schott & Endl., C. millenii and C.gigantea A. Chev. and C. acuminata suggest a probable closeness among these species. The results obtained in this study provide preliminary evidence of the chemotaxonomic significance of secondary metabolites and antimicrobial activities in infra-generic taxonomy of species of Cola.Key words: Cola species, Sterculiaceae, phytochemical screening, antimicrobial studies, taxonomy

PHYTOCHEMICAL AND ANTIMICROBIAL STUDIES OF FOUR SPECIES OF COLA SCHOTT & ENDL. (STERCULIACEAE)

The in-vitro antimicrobial evaluation of ethanol extracts of four species of Cola Schott & Endl. was done using human isolated strains of Staphylococcus aureus, Staphylococcus albus, Bacillus subtilis, Klebsiella pneumonia, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger as test organisms. The assays were carried out by agar well diffusion, erythromycin and ketoconazole served as the control drugs. The leaf ethanol extracts of the plants were found to be more effective against the tested fungi than the bacteria at high concentrations. None of the extracts was active against Staphylococcus aureus. Plant extract of C. acuminata (P. Beauv.) Schott & Endl. and C. nitida (Vent) Schott & Endl. showed activity on S. albus at concentrations ranging from 10-150 mgmlˉ¹ having comparable diameters of zone of inhibition of 7.3±0.03-16.0±0.0 for C. acuminata and 10.0±0.0-19.0±0.0 for C. nitida. Also, these two species of Cola demonstrated activities on C. albicans and A. niger at concentrations ranging from 90-150mgmlˉ¹ with relatively close diameters of zone of inhibition. Only C. acuminata inhibited the growth of K. pneumoniae at the MIC of 90mgmlˉ¹ whereas, C. albicans was inhibited by C. acuminata, C. millenii K. Schum and C. gigantea A.Chev. at the MIC of 120mgmlˉ¹. Phytochemical screening of the four species of Cola showed the presence of alkaloids, saponins, tannins and cardenolides in all the plants which apart from showing the probable closeness of the species could also be responsible for the observed activities. The antimicrobial property shown by the plant extracts is an evidence of the ethnomedicinal uses of the plants. The similarity observed in the phytochemical constituents and antimicrobial activities demonstrated by C nitida (Vent.) Schott & Endl., C. millenii and C.gigantea A. Chev. and C. acuminata suggest a probable closeness among these species. The results obtained in this study provide preliminary evidence of the chemotaxonomic significance of secondary metabolites and antimicrobial activities in infra-generic taxonomy of species of Cola

DETECTION AND SEROTYPING OF MAREKS DISEASE VIRUS IN DISEASED CHICKENS IN ABEOKUTA

Outbreaks of Marek’s disease (MD) in vaccinated flocks still occur sporadically and lead to economic losses. This study reports the detection of serotypes 1 and 3 Marek’s disease virus using molecular techniques in pullets showing clinical signs of the disease. An enzyme-linked immunosorbent assay (ELISA) was applied to evaluate the antibody response of chicks and the diseased pullets from the same parent stock against Marek’s disease (MD) at 4 and 21weeks of age respectively. Despite vaccination at day old, antibody titre in the chicks was negative while it was highly variable in the diseased pullets. From the results, the susceptibility of previously vaccinated birds to the pathogenic strain of MDV indicates inappropriate vaccination at day old and a call for concern in the poultry industry. It is therefore recommended that serology be carried out on birds to determine their immune status before and after MD vaccination. &nbsp

Indigenous Angiosperm biodiversity of Olabisi Onabanjo University permanent site

The conservation of the genetic variability of the indigenous angiosperm community is a sine qua non. A survey of indigenous angiosperm biodiversity of the Olabisi Onabanjo University permanent site was undertaken. Plants collected were dried, poisoned and mounted on herbarium sheets, proper identification and confirmation in recognized herbaria were carried out. A total number of one hundred and thirty-eight (138) plant species belonging to fifty-five (55) families were collected. Of these, one hundred and twenty-seven are dicotyledons and eleven are monocotyledons. Leguminosae is the largest family with thirteen plants followed by Rubiaceae with eleven and Euphorbiaceae with nine plants. Trees were found to have significantly contributed to the ecosystem with a total number of fifty-four species, while forty-three of shrubs were recorded, climbers ten, herbs twenty-eight, grasses and sedges three. From this study it is obvious that the University permanent site is not only rich in plant biodiversity but also very rich in socio-economic values. Consequently it is highly advisable that a representative sample of this vegetation is protected for posterity so that all the indigenous plants of the study area may not be lost to the development projects embarked upon by the University.African Journal of Biotechnology Vol. 4 (6), pp. 554-562, 200

Air quality index pattern around petroleum production facilities

Purpose: The aim of this paper is to better understand the impact of petroleum production facilities on ambient air quality of host airshed. Design/methodology/approach: Field measurements were taken daily for four consecutive months around petroleum production facilities in the Niger Delta area, of Nigeria, one of the world’s important petroleum producing areas. Statistical analysis tool and air quality analytical tool known as the air quality index (AQI) were applied on the field data obtained. Findings: The mean measured daily concentrations of both carbon monoxide (CO) and nitrogen dioxide (NO2) between distances 50 and 500 m of petroleum flow stations were of the range 140 – 3400 mg/m3 and 23 – 1250 µg/m3 respectively. The AQI from measured CO concentrations in the study area ranged between 1 and 44, an indication of good AQI category with no known health effects but a need for cautionary statement. Similarly, over 97 percent of the measured concentrations of NO2 were below 0.60 ppm which implies that the AQI of the host environment of the flow stations were below 200 with respect to NO2 thus indicating a good category of air with no health alarm. However, at the 60 m distance around a flow station, the AQI was 210 thus the quality of available air at this point could be described as very unhealthy. Generally the concentrations of CO were higher than NO2 in all the distances from the flow stations and were corroborated with their significant T-test values. The T-test results of the relationship between the concentrations of the air pollutants per time of the day, showed that their T-test values were not significant, indicating that concentrations of these air pollutants were independent of the sampling time. A strong and positive correlation existed between the two air pollutants signifying common sources.Originality/value: The paper highlights that at 60 m distance around petroleum production facilities, people with respiratory or heart disease, the elderly and children should be prevented from gaining access in the morning without taking necessary precautionary measures against the inhalation of air pollutants

REPRODUCTIVE FAILURES IN FOOD ANIMALS - A FIVE YEAR RETROSPECTIVE REVIEW

A total of 140 reproductive cases were recorded in food animals at the Teaching and Research farm, University of Agriculture, Abeokuta, between August, 2003 and May, 2008. Of these cases, the cattle, goat, sheep and piggery sections accounted for 14.3, 38.6, 10.7 and 36.4%, respectively. In the cattle section, cases recorded were, calf mortality (30.0%), agalactia (20.0%), mastitis (20.0%), still-birth (20.0%) and maternal mortality (10.0%). Cases recorded in the goat section were kid mortality (40.7%), abortion (24.0%), retained placenta (11.1%), mastitis (5.6%), maternal mortality (5.5%), still-birth (3.7%), paraphimosis (3.7%), agalactia (3.7%) and pyometra (1.9%). In sheep section however, cases recorded were lamb mortality (66.7%), uterine prolapse (6.7%), abortion (6.7%), retained placenta (6.7%), maternal mortality (6.7%) and metritis (6.7%). Cases recorded in the piggery section were piglet mortality (64.7%), infantophagia (7.8%), paraphimosis (5.9%), metritis (3.9%), mastitis (3.9%), stillbirth (2.0%), post parturient paralysis (2.0%), pyometra (2.0%), vesico-vagina fistula (2.0%), maternal mortality (2.0%), abortion (2.0%), and congenital eye defect (2.0%). In conclusion, good management practice, good environmental condition and genetic selection were identified as the bedrock for elimination and prevention of reproductive loss in food animals especially in the area of neonatal loss. Therefore, these three factors most critical in the sustenance of high productivity. &nbsp

Phytochemical screening, antimicrobial and antioxidant studies of Lannea egregia Engl. and K. Krause (Anacardiaceae) stem bark

Background: A substantial number of drugs are being developed from plants for the treatment of various diseases. Lannea egregia (LE) is a woody perennial plant used traditionally in the management of skin disorders and wounds. Aim: To investigate the biological activities of different solvent extracts of LE bark. Settings: Fresh stem bark of Lannea egregia was collected from Itabo Lanlate, Oyo State, Nigeria. The authentication was done in Forest Herbarium, Ibadan (FHI), Nigeria. The biological activities of the air-dried sample were carried out in the Department of Pharmacognosy, University of Ibadan, Nigeria. Materials and methods: Powdered LE bark sample was extracted by gradient extraction. Phytochemical screening was performed on the extracts using standard procedure. In vitro antimicrobial study was performed on 14 strains of bacteria and 5 fungal strains at a concentration range of 25, 50, 75 and 100 mg/mL of the extract using ciprofloxacin and itraconazole as standard. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) were determined. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and total phenolic content (TPC) of two active extracts were determined spectrophotometrically. Results: The phytochemical screening of LE revealed the presence of tannins, terpenoids, flavonoids, anthraquinones, saponins and alkaloids. The dichloromethane (DCM) extract exhibited the highest activity against all the bacterial strains as well as four of the fungal strains. The zones of inhibition (ZI) of bacteria ranged from 9.0 ± 2.0 to 24.6 ± 2.4 mm, MIC of 0.0008 to 12.5 mg/mL and MBC of 25 to 75 mg/mL, while ZI of the fungal strains ranged from 10.3 ± 4.6 to 18.0 ± 5.3 mm, MIC 0.391 – 0.781 and MFC of 50 mg/mL in all the strains. The TPC values of DCM and ethyl acetate (EtOAc) extracts were 1582.47 ± 6.69 and 1579.89 ± 12.77 µg GAE/mg and inhibitory antioxidant activity of 2.54 ± 0.58 and 2.44 ± 0.54, respectively. Conclusion: These findings provide scientific evidence to support the ethnomedicinal use of Lannea egregia bark for treating skin disorders and wounds

Environmental Impact Analysis of the Emission from Petroleum Refineries in Nigeria

Health and environmental hazards, a thing of global concern have been the major characteristics of the petroleum refinery areas worldwide, Nigeria inclusive. This is as a result of the emissions from petroleum refineries which resulted into air quality degradation of the host environment. This problem which has equally affected the climatic conditions of the petroleum producing areas is more pronounced in Nigeria due to lack of implementing adequate policies to protect the host environment. This study is carried out to investigate the atmospheric conditions of the petroleum refineries and identify the environmental impact of emissions of criteria pollutants from the proposed project in the area of influence. Emission inventory of criteria pollutants was carried out on the four existing and twenty-three proposed petroleum refineries in Nigeria. Using no control-measure option, the estimated annual criteria air pollutants emissions from point sources in the existing refineries are 1,217 tons/annum for PM10, 45,124 tons/annum for SO2, 167,570 tons/annum for NOx, 3,842 tons/annum for VOC and 242,469 tons/annum for CO. An additional 1,082 tons/annum of PM10, 168,944 tons/annum of SO2, 688,687 tons/annum of NOx, 9,122 tons/annum of VOC and 569,975 tons/annum of CO were predicted to be added into the Nigeria airshed by the proposed petroleum refineries. The highest pollutant emitting state was predicted to be Rivers State with the highest number of refineries while the least pollutant emitting states were predicted to be Kaduna, Edo, Lagos and Anambra States with only one refinery in each of the state. The ability to adopt appropriate control measures will determine the rate of emission of criteria pollutants released into the country’s airshed

Modeling of Criteria Air Pollutant Emissions from Selected Nigeria Petroleum Refineries

The dispersion models were used to estimate or predict the concentration of air pollutants or toxins emitted from sources such as industrial plants, vehicular traffic or accidental chemical releases. In this study, the Industrial Source Complex Short Term (ISCST3) emission dispersion model was used to measure the ground level concentration of criteria air pollutants within 50 km radius of location. This model considered emissions from major point sources of pollutants in four existing and twenty-three proposed Nigeria petroleum refineries. The obtained ground level concentration for 24-hr averaging periods of the criteria air pollutants at sensitive receptor around each of the refineries was compared with the National Ambient Air Quality Standards (NAAQS) of Nigeria, World Bank and World Health Organization (WHO) to determine their level of compliance. The highest ground level concentration predicted to be 450 - 1875 μg/m3 for 24-h averaging period was obtained at Tonwei Oil Refinery, Ekeremor Local Government, Bayelsa State, while the lowest ground level concentration predicted to be 0.0099 - 0.1 μg/m3 for 24-h averaging period was obtained at Amakpe International Refinery, Eket Local Government, Akwa Ibom State. Percentage set limits of criteria air pollutants ranging from 0.02% to 94.5% are within the set standard limits and no health risk is associated with areas around the plant’s locations while percentage set limits of criteria pollutants ranging from 1.1 to 55.6 folds of the standards exceed the maximum permitted limits, hence affecting areas around the plants. The air quality standards guiding petroleum refinery emissions must be strictly considered, in order to ensure that the ground level concentrations do not exceed the required standard limits and prevent the adverse effects of air pollution in the Nigeria airshed