Effects of Low-Level Laser Therapy and Eccentric Exercises in the Treatment of Patellar Tendinopathy

The study aims to investigate if low-level laser therapy (LLLT) combined with eccentric exercises could more effectively treat patellar tendinopathy than LLLT alone and eccentric exercises alone. Twenty-one patients with patellar tendinopathy were randomized to three groups: laser alone, exercise alone, or laser plus exercise, with seven in each group. Laser irradiations were administered at the inferior pole of the patella and the two acupoints of Extra 36 (Xiyan) with the intensity of 1592 mW/cm2. Eccentric training program consisted of three sets of 15 repetitions of unilateral squat on level ground. All patients received six treatments per week for four weeks. Knee pain and function and quadriceps muscle strength and endurance were evaluated at baseline and the end of treatment. After the 4-week intervention, all groups showed significant improvements in all the outcomes (P<0.01). The laser + exercise group had significantly greater improvements in all the outcomes than the other two groups (P<0.05), except nonsignificant difference in pain relief between the laser + exercise group and the laser group. In conclusion, LLLT combined with eccentric exercises is superior to LLLT alone and eccentric exercises alone to reduce pain and improve function in patients with patellar tendinopathy

Mechanism underlying synergic activation of Tyrosinase promoter by MITF and IRF4

Background: The transcription factor interferon regulatory factor 4 (IRF4) was identified to be involved in human pigmentation by genome-wide association studies (GWASs). The rs12203592-[T/C], which is located in intron 4 of IRF4, shows the strongest link to these pigmentation phenotypes including freckling, sun sensitivity, eye and hair color. Previous studies indicated a functional cooperation of IRF4 with Microphthalmia-associated transcription factor (MITF), a causing gene of Waardenburg syndrome (WS), to synergistically trans-activate Tyrosinase (TYR). However, the underlying mechanism is still unknown. Methods: To investigate the importance of DNA binding in the synergic effect of IRF4. Reporter plasmids with mutant TYR promoters was generated to locate the IRF4 DNA binding sites in the Tyrosinase minimal promoter. By building MITF and IRF4 truncated mutations plasmids, the necessary regions of the synergy functions of these two proteins were also located. Results: The cooperative effect between MITF and IRF4 was specific for TYR promoter. The DNA-binding of IRF4 was critical for the synergic function. IRF4 DNA binding sites in TYR promoter were identified. The Trans-activation domains in IRF4 (aa134-207, aa300-420) were both important for the synergic function, whereas the auto-mask domain (aa207-300) appeared to mask the synergic effect. Mutational analysis in MITF indicated that both DNA-binding and transcriptional activation domains were both required for this synergic effect. Conclusions: Here we showed that IRF4 potently synergized with MITF to activate the TYR promoter, which was dependent on DNA binding of IRF4. The synergic domains in both IRF4 and MITF were identified by mutational analysis. This identification of IRF4 as a partner for MITF in regulation of TYR may provide an important molecular function for IRF4 in the genesis of melanocytes and the pathogenic mechanism in WS

Changes of plasma fibroblast growth factor-21 (FGF-21) in oral glucose tolerance test and effects of metformin on FGF-21 levels in type 2 diabetes mellitus

Wstęp: Badanie przeprowadzono w celu ustalenia, czy czynnik wzrostu fibroblastów-21 (FGF-21) uczestniczy w regulacji stężenia glukozy i czy zastosowanie metforminy u chorych na cukrzycę wpływa na stężenie FGF-21. Materiał i metody: Do badania włączono 43 osoby, w tym 27 chorych z nowo rozpoznaną cukrzycą typu 2 (nT2DM). U wszystkich przeprowadzono test doustnego obciążenia 75 g glukozy (OGTT). Próbki krwi pobrano w 0., 60.,120. i 180. minucie testu. Osobom z nT2DM zaproponowano udział w dalszych badaniach; zastosowano u nich metforminę w dawce 1,0 g/dobę przez tydzień. Wyniki: Zmiany stężenia FGF-21 w osoczu podczas OGTT zaobserwowano tylko w grupie chorych na nT2DM; w grupie kontrolnej stężenie FGF-21 pozostało niezmienione. Nie stwierdzono, by stężenia FGF-21 w poszczególnych punktach czasowych różniły się w zależności od płci badanych (p < 0,05). Zastosowanie metforminy u osób z nT2DM spowodowało istotne zmniejszenie stężeń glukozy i FGF-21 we wszystkich punktach czasowych OGTT oraz zmniejszenie stężenia insuliny w 60. i 180. minucie, co wskazuje na obniżenie wskaźnika HOMA-IR. Wnioski: FGF-21 nie uczestniczy w krótkoterminowej regulacji glikemii u ludzi, a zmiany jego stężenia podczas OGTT są opóźnione w T2DM. Być może FGF-21 bierze udział w metabolizowaniu metforminy, zwiększając wrażliwość na glukozę i insulinę. (Endokrynol Pol 2013; 64 (3): 220&#8211;224)Introduction: The objectives of our study were to investigate whether fibroblast growth factor-21 (FGF-21) is involved in short-term regulation of glucose and the change of FGF-21 after metformin use in diabetic subjects. Material and methods: 43 subjects were recruited in the study, including 27 new-onset type 2 diabetes patients (nT2DM). A 75 g oral glucose tolerance test (OGTT) was administered to them. Blood samples were taken at 0, 60 ,120 and 180 minute of OGTT. nT2DM subjects were invited for further investigation, metformin was administered in a dose of 1.0 g every day for 1 week. Results: Plasma FGF-21 changed significantly in the nT2DM group during the OGTT administration but not in the control group. No gender differences were observed at different time points in FGF-21 levels (p < 0.05). Administration of metformin for nT2DM resulted in a significant decrease in both glucose and FGF-21 at all OGTT times and in insulin at 60 min and 180 min, indicative of a decrease in HOMA-IR. Conclusion: FGF-21 does not seem to be involved in short-term regulation of glycaemia in human subjects, and the change in OGTT delayed in T2DM. FGF-21 may participate in the processing of metformin, improving glucose and insulin sensitivity. (Pol J Endocrinol 2013; 64 (3): 220&#8211;224

Tree-ring-based precipitation reconstruction in the source region of Weihe River, northwest China since AD 1810

A tree-ring width chronology of Picea purpurea Mast from Mt. Shouyang in the source region of Weihe River (SWR), northwest China, was developed in this study. Correlation analysis showed that the precipitation from previous August to current July was the limiting climate factor of tree growth. Using a reliable and stable linear regression model, which explained 42.6% of the variance of the actual precipitation during the calibration period from 1958 to 2014, a 205-year long precipitation series was reconstructed for the SWR. The dry years in the reconstruction were well supported by historical documents, and famous historical droughts were also recorded in the dry periods of a low-frequency scale of the reconstructed precipitation. As demonstrated by the spatial correlation patterns, the reconstructed series compared well with other hydroclimate records for northwest China, indicating that it could represent large-scale hydroclimate changes. The 2-8-year interannual cycles and the interdecadal quasiperiods of 15.9 years and 18.6 years revealed that the precipitation in this region was probably affected by the El Nino-Southern Oscillation and North Atlantic Oscillation. The dry/wet years corresponded well with the El Nino/La Nina events and the SWR commonly experienced droughts during the low periods of North Atlantic Oscillation

Preparation of FeO(OH) Modified with Polyethylene Glycol and Its Catalytic Activity on the Reduction of Nitrobenzene with Hydrazine Hydrate

Iron oxyhydroxide was prepared by dropping ammonia water to Fe(NO3)3.9H2O dispersed in polyethylene glycol (PEG) 1000. The catalyst was characterized by X-ray powder diffraction, Fourier transform infrared spectroscopy and laser particle size analyzer. The results showed the catalyst modified with polyethylene glycol was amorphous. The addition of PEG during the preparation make the particle size of the catalyst was smaller and more uniform. The catalytic performance was tested in the reduction of nitroarenes to corresponding amines with hydrazine hydrate, and the catalyst showed excellent activity and stability.

An Evaluation on Bridge Bearing Capacity under Scour and Re-occurrence of Strong Earthquake

Plagued by frequent calamities, Bridge No.3 encountered magnitude-8 earthquake on May 12, 2008 and several years later its pile foundation was intensively scoured. The smallest scour depth was 4.5 meters and the largest scour depth was 9.2 meters. Considering intense scour and re-occurrence of strong earthquake, the Chinese existing standard and seismic response analysis are used to study bearing capacity and seismic performance of pier and pile foundation of Bridge No.3 before and after scour. It is proved by calculation that the bridge is stable before scour and can hardly bear strong earthquake and intense scour after scour, therefore consolidation is required. The study result may serve as an important reference for the bridge affected by serious scour and strong earthquake

Improving Autonomous Driving Safety with POP: A Framework for Accurate Partially Observed Trajectory Predictions

Accurate trajectory prediction is crucial for safe and efficient autonomous driving, but handling partial observations presents significant challenges. To address this, we propose a novel trajectory prediction framework called Partial Observations Prediction (POP) for congested urban road scenarios. The framework consists of two key stages: self-supervised learning (SSL) and feature distillation. POP first employs SLL to help the model learn to reconstruct history representations, and then utilizes feature distillation as the fine-tuning task to transfer knowledge from the teacher model, which has been pre-trained with complete observations, to the student model, which has only few observations. POP achieves comparable results to top-performing methods in open-loop experiments and outperforms the baseline method in closed-loop simulations, including safety metrics. Qualitative results illustrate the superiority of POP in providing reasonable and safe trajectory predictions