Copper-Promoted Reductive Coupling of Aryl Iodides with 1,1,1-Trifluoro-2-iodoethane

An efficient Cu-promoted reductive coupling of aryl iodides with 1,1,1-trifluoro-2-iodoethane has been developed. This reaction could occur in good yields under milder conditions as compared with previous studies. The reaction tolerated nitro, formyl, ester, ether, carbonyl, sulfonyl, and even azo groups

Dual colorimetric and fluorescent determination of iron (III) using a novel squaraine dye

<p>A novel squaraine dye, 6-carboxy-2-[[3-[1,3-dihydro-3,3-dimethy-1-ethyl-2<i>H</i>-indol-2-ylidene)methyl]-2-hydroxy-4-oxo-2-cyclobuten-1-ylidene]methyl]-3,3-trimethy-3<i>H</i>-indolium, has been synthesized. The squaraine dye was found to be a dual colorimetric and fluorescent probe for the determination of iron (III) in CH₃CH₂OH/H₂O (4:1, v/v) exhibiting high selectivity and sensitivity. The binding of squaraine dye +Fe³⁺ was studied by a Job’s plot and Fourier transform infrared and ¹H nuclear magnetic resonance spectroscopies. The result indicates that the squaraine dye may be utilized as a naked-eye and real-time probe for the efficient determination of Fe^3+.</p

Magnetic Nano-Fe₃O₄-Supported 1-Benzyl-1,4-dihydronicotinamide (BNAH): Synthesis and Application in the Catalytic Reduction of α,β-Epoxy Ketones

A novel magnetically recoverable organic hydride compound was successfully constructed by using silica-coated magnetic nanoparticles as a support. An as-prepared magnetic organic hydride compound, BNAH (1-benzyl-1,4-dihydronicotinamide), showed efficient activity in the catalytic reduction of α,β-epoxy ketones. After reaction, the magnetic nanoparticle-supported BNAH can be separated by simple magnetic separation which made the separation of the product easier

Table_1_Traits-Based Integration of Multi-Species Inoculants Facilitates Shifts of Indigenous Soil Bacterial Community.DOCX

<p>Microbial co-inoculation is considered to be an innovative approach and had been applied worldwide. However, the underlying mechanisms of microbial co-inoculants constructions, especially the trait-based combination of distinctly different microbial species remains poorly understood. In this study, we constructed two microbial co-inoculants with the same three strains with emphasis on the microbial, soil and plant traits. Microbial co-inoculants 1 (M1) were constructed according to soil fertility, microbial activity and cucumber nutrient requirement with a 2:1:2 ratio (Ensifer sp. NYM3, Acinetobacter sp. P16 and Flavobacterium sp. KYM3), while microbial co-inoculants 2 (M2) were constructed according to soil fertility and cucumber nutrient requirement with a 1:10:1 ratio without considering the difference in the nutrient supply capability of microbial species. The results showed that M1 and M2 both obviously increased cucumber yields. The M1 had significant highest pH value, total nitrogen (TN) and invertase activity (IA). The M2 had significant highest available phosphate (AP), NO₃-N, urea activity (UA), and alkaline phosphatase activity (APA). Gammaproteobacteria, Acidobacteria, Nitrospirae, and Armatimonadetes were significantly increased, while Actinobacteria and Firmicutes were significantly decreased by microbial co-inoculations (M1 and M2). The bacterial lineages enriched in M1 were Gammaproteobacteria and TM7. Acidobacteria, Bacteroidetes, and Deltaproteobacteria were enriched in M2. Principal coordinate analysis (PCoA) analysis showed that the bacterial communities were strongly separated by the different microbial inoculation treatments. The functional groups of intracellular_parasites were highest in M1. The functional groups of phototrophy, photoautotrophy, nitrification, fermentation, cyanobacteria, oxygenic_photoautotrophy, chitinolysis and animal_parasites_or_symbionts were highest in M2. Based on correlation analysis, it inferred that the M1 and M2 might promote cucumber yields by mediating bacterial community structure and function about nitrogen fixing and urea-N hydrolysis, respectively. Collectively, these results revealed that microbial co-inoculants had positive effects on cucumber yields. Trait-based integration of different microbial species had significant effects on soil properties and bacterial communities. It indicated that microbial activity should be considered in the construction of microbial co-inoculants. This will expand our knowledge in bacteria interaction, deepen understanding of microbial inoculants in improving plant performance, and will guide microbial fertilizer formulation and application in future.</p

Low-Coordinate Iron(II) Complexes of a Bulky Bis(carbene)borate Ligand

The bulky bis­(carbene)­borate ligand H₂B­(^tBuIm)₂^– allows for the synthesis of three- and four-coordinate iron­(II) complexes, including heteroleptic H₂B­(^tBuIm)₂FeN­(TMS)₂ and homoleptic [H₂B­(^tBuIm)₂]₂Fe. The magnetic properties of these coordinatively unsaturated complexes have been characterized by SQUID magnetometry, but no evidence of single-molecule magnet behavior is observed, despite large negative uniaxial zero field splitting. The three-coordinate complex H₂B­(^tBuIm)₂FeN­(TMS)₂ serves as a precursor for the synthesis of the four-coordinate mixed carbene complex H₂B­(^tBuIm)₂(ⁱPr₂Im)­FeCl, which has a coordination environment similar to that found in tris­(carbene)­borate iron­(II) chloride complexes. Despite this similarity, attempts to prepare the corresponding iron­(IV) nitride were unsuccessful, suggesting that subtle structural factors are critical to stabilizing this species

Table_2_Traits-Based Integration of Multi-Species Inoculants Facilitates Shifts of Indigenous Soil Bacterial Community.DOCX

<p>Microbial co-inoculation is considered to be an innovative approach and had been applied worldwide. However, the underlying mechanisms of microbial co-inoculants constructions, especially the trait-based combination of distinctly different microbial species remains poorly understood. In this study, we constructed two microbial co-inoculants with the same three strains with emphasis on the microbial, soil and plant traits. Microbial co-inoculants 1 (M1) were constructed according to soil fertility, microbial activity and cucumber nutrient requirement with a 2:1:2 ratio (Ensifer sp. NYM3, Acinetobacter sp. P16 and Flavobacterium sp. KYM3), while microbial co-inoculants 2 (M2) were constructed according to soil fertility and cucumber nutrient requirement with a 1:10:1 ratio without considering the difference in the nutrient supply capability of microbial species. The results showed that M1 and M2 both obviously increased cucumber yields. The M1 had significant highest pH value, total nitrogen (TN) and invertase activity (IA). The M2 had significant highest available phosphate (AP), NO₃-N, urea activity (UA), and alkaline phosphatase activity (APA). Gammaproteobacteria, Acidobacteria, Nitrospirae, and Armatimonadetes were significantly increased, while Actinobacteria and Firmicutes were significantly decreased by microbial co-inoculations (M1 and M2). The bacterial lineages enriched in M1 were Gammaproteobacteria and TM7. Acidobacteria, Bacteroidetes, and Deltaproteobacteria were enriched in M2. Principal coordinate analysis (PCoA) analysis showed that the bacterial communities were strongly separated by the different microbial inoculation treatments. The functional groups of intracellular_parasites were highest in M1. The functional groups of phototrophy, photoautotrophy, nitrification, fermentation, cyanobacteria, oxygenic_photoautotrophy, chitinolysis and animal_parasites_or_symbionts were highest in M2. Based on correlation analysis, it inferred that the M1 and M2 might promote cucumber yields by mediating bacterial community structure and function about nitrogen fixing and urea-N hydrolysis, respectively. Collectively, these results revealed that microbial co-inoculants had positive effects on cucumber yields. Trait-based integration of different microbial species had significant effects on soil properties and bacterial communities. It indicated that microbial activity should be considered in the construction of microbial co-inoculants. This will expand our knowledge in bacteria interaction, deepen understanding of microbial inoculants in improving plant performance, and will guide microbial fertilizer formulation and application in future.</p

Table_1_An anoikis-related gene signature for prediction of the prognosis in prostate cancer.docx

PurposeThis study presents a novel approach to predict postoperative biochemical recurrence (BCR) in prostate cancer (PCa) patients which involves constructing a signature based on anoikis-related genes (ARGs).MethodsIn this study, we utilised data from TCGA-PARD and GEO databases to identify specific ARGs in prostate cancer. We established a signature of these ARGs using Cox regression analysis and evaluated their clinical predictive efficacy and immune-related status through various methods such as Kaplan-Meier survival analysis, subject work characteristics analysis, and CIBERSORT method. Our findings suggest that these ARGs may have potential as biomarkers for prostate cancer prognosis and treatment. To investigate the biological pathways of genes associated with anoikis, we utilised GSVA, GO, and KEGG. The expression of ARGs was confirmed by the HPA database. Furthermore, we conducted PPI analysis to identify the core network of ARGs in PCa.ResultsBased on analysis of the TCGA database, a set of eight ARGs were identified as prognostic signature genes for prostate cancer. The reliability and validity of this signature were well verified in both the TCGA and GEO codifications. Using this signature, patients were classified into two groups based on their risk for developing BCR. There was a significant difference in BCR-free time between the high and low risk groups (P ConclusionThis signature suggests the potential role of ARGs in the development and progression of PCa and can effectively predict the risk of BCR in PCa patients after surgery. It also provides a basis for further research into the mechanism of ARGs in PCa and for the clinical management of patients with PCa.</p

Restoration of miR-1228* Expression Suppresses Epithelial-Mesenchymal Transition in Gastric Cancer

<div><p>Dysregulated miRNAs play critical roles during carcinogenesis and cancer progression. In the present study, the function of miR-1228* in regulating cancer progression was investigated in gastric cancer. Decreased expression of miR-1228* was observed in human gastric cancer tissues comparing to normal tissues. Subsequently, the role of miR-1228* was evaluated <i>in vivo</i> using the tumor xenograft model. In this model, miR-1228* overexpression suppressed xenograft tumor formation. Furthermore, we demonstrated miR-1228* negatively regulated NF-κB activity in SGC-7901 gastric cancer cells and found that CK2A2 was a target of miR-1228*. Upregulation of miR-1228* decreased the expression of mesenchymal markers and increased the epithelial marker E-cadherin, suggesting its potential role in suppressing epithelial-mesenchymal transition. Collectively, these findings provide the first evidence that miR-1228* plays an important role in regulating gastric cancer growth and suggest that selective restoration of miR-1228* might be beneficial for gastric cancer therapy.</p> </div

Ligand Substituent Effects in Manganese Pyridinophane Complexes: Implications for Oxygen-Evolving Catalysis

A series of Mn­(II) complexes of differently substituted pyridinophane ligands, (Py₂NR₂)­MnCl₂ (R = <i>ⁱ</i>Pr, Cy) and [(Py₂NR₂)­MnF₂]­(PF₆) (R = <i>ⁱ</i>Pr, Cy, <i>^t</i>Bu) are synthesized and characterized. The electrochemical properties of these complexes are investigated by cyclic voltammetry, along with those of previously reported (Py₂NMe₂)­MnCl₂ and the Mn­(III) complex [(Py₂NMe₂)­MnF₂]­(PF₆). The electronic structure of this and other Mn­(III) complexes is probed experimentally and theoretically, via high-frequency and -field electron paramagnetic resonance (HFEPR) spectroscopy ab initio quantum chemical theory (QCT), respectively. These studies show that the complexes contain relatively typical six-coordinate Mn­(III). The catalytic activity of these complexes toward both H₂O₂ disproportionation and H₂O oxidation has also been investigated. The rate of H₂O₂ disproportionation decreases with increasing substituent size. Some of these complexes are active for electrocatalytic H₂O oxidation; however this activity cannot be rationalized in terms of simple electronic or steric effects

Changes of liver function (AST and ALT) and kidney function (BUN and CRE) on day 9 and day 12 after the intraperitoneal injection of LPS.

(A)—(D) Changes of liver function and kidney function on the 9th day. (E)—(H) Changes of liver function and kidney function on the 12th day. The data were shown as mean ± S.E.M with 5~9 rats in each group. # and ##, compared with normal groups, P P P P < 0.01. NS: no significance.</p