Ghost-removal image warping for optical flow estimation

Traditional image warping methods used in optical flow estimation usually adopt simple interpolation strategies to obtain the warped images. But without considering the characteristic of occluded regions, the traditional methods may result in undesirable ghosting artifacts. To tackle this problem, in this paper we propose a novel image warping method to effectively remove ghosting artifacts. To be Specific, when given a warped image, the ghost regions are firstly discriminated using the optical flow information. Then, we use a new image compensation technique to eliminate the ghosting artifacts. The proposed method can avoid serious distortion in the warped images, therefore can prevent error propagation in the coarse-to-fine optical flow estimation schemes. Meanwhile, our approach can be easily integrated into various optical flow estimation methods. Experimental results on some popular datasets such as Flying Chairs and MPI-Sintel demonstrate that the proposed method can improve the performance of current optical flow estimation methods

Nitrogen loss in vegetable field under the simulated rainfall experiments in Hebei, China

Agricultural non-point source pollution is one of the main factors contaminating the environment. However, the impact of rainfall on loss of non-point nitrogen is far from well understood. Based on the artificial rainfall simulation experiments to monitor the loss of dissolved nitrogen (DN) in surface runoff and interflow of vegetable field, this study analyzed the effects of rainfall intensity and fertilization scheme on nitrogen (N) loss. The results indicated that fertilizer usage is the main factor affecting the nitrogen loss in surface runoff, while runoff and rainfall intensity play important roles in interflow nitrogen loss. The proportion of DN lost through the surface runoff was more than 91%, and it decreased with increasing rainfall intensity. There was a clear linear trend (r2 > 0.96) between the amount of DN loss and runoff. Over 95% of DN was lost as nitrate nitrogen (NN), which was the major component of nitrogen loss. Compared with the conventional fertilization treatment (CF), the amount of nitrogen fertilizer applied in the optimized fertilization treatment (OF) decreased by 38.9%, and the loss of DN decreased by 28.4%, but root length, plant height and yield of pak choi increased by 6.3%, 2.7% and 5.6%, respectively. Our findings suggest that properly reducing the amount of nitrogen fertilizer can improve the utilization rate of nitrogen fertilizer but will not reduce the yield of pak choi. Controlling fertilizer usage and reducing runoff generation are important methods to reduce the DN loss in vegetable fields

Optical Flow Estimation with Occlusion Detection

The dense optical flow estimation under occlusion is a challenging task. Occlusion may result in ambiguity in optical flow estimation, while accurate occlusion detection can reduce the error. In this paper, we propose a robust optical flow estimation algorithm with reliable occlusion detection. Firstly, the occlusion areas in successive video frames are detected by integrating various information from multiple sources including feature matching, motion edges, warped images and occlusion consistency. Then optimization function with occlusion coefficient and selective region smoothing are used to obtain the optical flow estimation of the non-occlusion areas and occlusion areas respectively. Experimental results show that the algorithm proposed in this paper is an effective algorithm for dense optical flow estimation

The diffuse reduction in spleen density: an indicator of severe acute pancreatitis?

Correspondence : Xuewei Bai ([email protected]) We observed that acute pancreatitis (AP) was associated with diffuse reduction in spleen density (DROSD) in some patients. Furthermore, the condition of these patients was more serious, and the potential relationship between DROSD and structural and functional injury of the spleen remained unclear. Therefore, we performed a preliminary exploration of these factors. We analysed pertinent clinical data for AP patients with normal spleen density (control group) and for those with DROSD (reduction group) at the First Affiliated Hospital of Harbin Medical University (June 2013-June 2015). We measured the immunoglobulin M (IgM) B-cells of the AP patients and examined pancreatic and splenic tissues from AP rats with optical microscopy and TEM. The reduction group had a higher acute physiology and chronic health evaluation II (APACHE II) score, a longer length of stay (LOS) and lower serum calcium than the control group. The levels of triglycerides (TG) and total cholesterol (TC) did not differ significantly between the two groups. The percentage of IgM memory B-cells was significantly lower in the DROSD group than in the control group. TEM revealed that the spleen T-lymphocytes were normal in AP rats, but pyroptotic and necrotic spleen B-cells were observed in the severe AP rats. In AP, DROSD was an independent indicator of more severe conditions. Furthermore, spleen B-lymphocytes showed obvious damage at the cellular level, and the immunological function of the spleen was down-regulated when AP was associated with DROSD

Attenuation of Aeromonas hydrophila Infection in Carassius auratus by YtnP, a N-acyl Homoserine Lactonase from Bacillus licheniformis T-1

In this experiment, the quorum quenching gene ytnP of Bacillus licheniformis T-1 was cloned and expressed, and the effect against infection of Aeromonas hydrophila ATCC 7966 was evaluated in vitro and vivo. The BLAST results revealed a 99% sequence identity between the ytnP gene of T-1 and its homolog in B.subtilis sub sp. BSP1, and the dendroGram showed that the similarity in the YtnP protein in T-1 was 100% in comparison with B.subtilis 3610, which was categorized as the Aidc cluster of the MBL family. The AHL lactonase activity of the purified YtnP was detected as 1.097 ± 0.7 U/mL with C6-HSL as the substrate. Otherwise, purified YtnP protein could significantly inhibit the biofilm formation of A.hydrophila ATCC 7966 with an inhibition rate of 68%. The MIC of thiamphenicol and doxycycline hydrochloride against A. hydrophila reduced from 4 μg/mL and 0.5 μg/mL to 1 μg/mL and 0.125 μg/mL, respectively, in the presence of YtnP. In addition, YtnP significantly inhibited the expression of five virulence factors hem, ahyB, ast, ep, aerA of A. hydrophila ATCC 7966 as well (p < 0.05). The results of inhibition on virulence showed a time-dependence tendency, while the strongest anti-virulence effects were within 4–24 h. In vivo, when the YtnP protein was co-injected intraperitoneally with A. hydrophila ATCC 7966, it attenuated the pathogenicity of A. hydrophila and the accumulated mortality was 27 ± 4.14% at 96 h, which was significantly lower than the average mortality of 78 ± 2.57% of the Carassius auratus injected with 108 CFU/mL of A. hydrophila ATCC 7966 only (p < 0.001). In conclusion, the AHL lactonase in B. licheniformis T-1 was proven to be YtnP protein and could be developed into an agent against infection of A. hydrophila in aquaculture

<title>Initital research of three-dimensional volumetric display based on di-frequency upconversion</title>

Three-dimensional display may be one of the most wonderful techniques attracting scientists to research on. But most of current approaches are fictitious ones. On the CLEO&apos;94, E. A. Downing and L. Hesselink et al. reported a virtual three-dimensional volumetric display using Pr doped fluoride glass upconversion luminescence which is evaluated as a new excellent physics achievement in 1996. The ZBLAN:Pr glass is proved by L. Hesselink to be a interesting material for three-dimensional volumetric display based on upconversion. In the processes of upconverting near-infrared radiation to visible, the 1G4 level of Pr3+ locates at an important position. But the transition probability of 3H4-1G4 proved to be one or two order of magnitude smaller than other strong transitions. So to improve the population on 1G4 has an practical significance. In this paper, we report a simple 3-D volumetric display experiment which has used the upconversion sensitization method. A bulk ZBLAN:Pr,Yb and ZBLAN:Pr glasses were selected as display medium. Just as the sensitized action of Yb3+ ion, the di-frequency upconversion luminescence intensity of Pr3+ ion is enhanced more than thirty times. So, the brightness of 3-D display is better than the work of E. A. Downing and L. Hesselink. Some technique consideration is discussed. In addition a conception of multilayer 3-D display device is also presented, which are thought having several advantages.EI

circEIF3I facilitates the recruitment of SMAD3 to early endosomes to promote TGF-β signalling pathway-mediated activation of MMPs in pancreatic cancer

Abstract Background Among digestive tract tumours, pancreatic ductal adenocarcinoma (PDAC) shows the highest mortality trend. Moreover, although PDAC metastasis remains a leading cause of cancer-related deaths, the biological mechanism is poorly understood. Recent evidence demonstrates that circular RNAs (circRNAs) play important roles in PDAC progression. Methods Differentially expressed circRNAs in normal and PDAC tissues were screened via bioinformatics analysis. Sanger sequencing, RNase R and actinomycin D assays were performed to confirm the loop structure of circEIF3I. In vitro and in vivo functional experiments were conducted to assess the role of circEIF3I in PDAC. MS2-tagged RNA affinity purification, mass spectrometry, RNA immunoprecipitation, RNA pull-down assay, fluorescence in situ hybridization, immunofluorescence and RNA–protein interaction simulation and analysis were performed to identify circEIF3I-interacting proteins. The effects of circEIF3I on the interactions of SMAD3 with TGFβRI or AP2A1 were measured through co-immunoprecipitation and western blotting. Results A microarray data analysis showed that circEIF3I was highly expressed in PDAC cells and correlated with TNM stage and poor prognosis. Functional experiments in vitro and in vivo revealed that circEIF3I accelerated PDAC cells migration, invasion and metastasis by increasing MMPs expression and activity. Mechanistic research indicated that circEIF3I binds to the MH2 domain of SMAD3 and increases SMAD3 phosphorylation by strengthening the interactions between SMAD3 and TGFβRI on early endosomes. Moreover, AP2A1 binds with circEIF3I directly and promotes circEIF3I-bound SMAD3 recruitment to TGFβRI on early endosomes. Finally, we found that circEif3i exerts biological functions in mice similar to those of circEIF3I in humans PDAC. Conclusions Our study reveals that circEIF3I promotes pancreatic cancer progression. circEIF3I is a molecular scaffold that interacts with SMAD3 and AP2A1 to form a ternary complex, that facilitates the recruitment of SMAD3 to early endosomes and then activates the TGF-β signalling pathway. Hence, circEIF3I is a potential prognostic biomarker and therapeutic target in PDAC