Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook

In the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. elwesii Hook. A simple method for the extraction of lycorine in low mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut(r)). The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:92.5:7.5, v/v/v) applied at a flow rate 1 mL min-1 using diode array detector. The content of lycorine in the bulbs and aerial parts of G. elwesii collected from Demirci (Manisa) was found as 0.130 and 0.162 %, respectively. Additionally, in the bulbs of the specimens collected from Sogucak (Balikesir), lycorine was quantified as 0.055 %, whereas in the aerial parts, it was determined as 0.006 %. The method was validated partially with respect to system specificity, linearity, accuracy, precision, limits of detection (LOD) and quantitation (LOQ). Validation procedures displayed that the method was specific, accurate and precise

Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD

WOS: 000444045400003In this study, a reversed-phase, high pressure liquid chromatography (RP-HPLC) method was applied in the quantitative analysis of lycorine in the aerial parts and bulbs of Galanthus alpinus Sosn. var. alpinus collected from Rize during the flowering and fruiting seasons. A simple method to extract lycorine from the plant specimens was performed, using pre-packed columns with diatomaceous earth (Extrelut (R)). The chromatographic separation was achieved employing an isocratic system with a mobile phase including trifluoroacetic acid -water-acetonitrile (0.01:95:51 measured at a flow rate of 1 ml/min using a diode array detector (DAD). The lycorine content in the bulbs during the flowering and fruiting seasons was detected as 0.01576 and 0.02351 %, respectively. Also, validation studies showed that the method was specific, accurate and precise.Ege University Research FundEge University [2014/ECZ/038]The financial support of Ege University Research Fund (project number 2014/ECZ/038) was acknowledged

QUANTITATIVE DETERMINATIONS ON GALANTHUS RIZEHENSIS

Galanthus rizehensis Stern (Amaryllidaceae) was collected from Maçka, Trabzon during flowering and fruiting seasons. Quality control studies and quantitative determination of lycorine were carried out on the prepared specimens. In this respect, assays for humidity, total ash, sulphated ash, hydrochloric acid-insoluble ash were performed on each drug specimen. The total alkaloidal content of G. rizehensis was determined by a titrimetric method. Furthermore, the identification and quantitative determination of lycorine on the aerial and underground parts of the plant collected during two different seasons were performed by using a HPLC-DAD method.Galanthus rizehensis Stern (Amaryllidaceae) bitkisi çiçekli ve meyveli dönemde Maçka, Trabzon'dan toplanmıştır. Hazırlanan örnekler üzerinde kalite kontrol çalışmaları ve likorin miktar tayini yapılmıştır. Bu amaçla, herbir örnek üzerinde, nem, total kül, sülfat külü ve hidroklorik asitte çözünmeyen kül deneyleri gerçekleştirilmiştir. G. rizehensis bitkisinin total alkaloit içeriği ise titrimetrik esaslı bir yöntemle tayin edilmiştir. Ayrıca, iki farklı zamanda toplanmış olan bitkinin toprak üstü ve toprak altı kısımlarındaki likorinin teşhisi ve miktar tayini, YBSK-DAD yöntemi kullanılarak yapılmıştır

HPLC - DAD Analysis of Lycorine in Amaryllidaceae Species

WOS: 000278891800010PubMed ID: 20614813Lycorine, the most frequent alkaloid found in Amaryllidaceae plants, has been proven to have various biological activities. Therefore, it is important to quantify this compound in Amaryllidacaeae species. In this study, a reversed-phase high-performance liquid chromatographic method has been used and validated for the determination of lycorine in Amaryllidaceae plants. A simple method for the extraction of lycorine in low-mass plant samples was employed utilizing columns pre-packed with diatomaceous earth (Extrelut (R)). This method was applied to the aerial parts and bulbs of Sternbergia sicula Tineo ex Guss., S. lutea (L.) Ker-Gawl. ex Sprengel and Pancratium maritimum L. (Amaryllidaceae) collected during two different vegetation periods. The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:90:10) applied at a flow rate 1 mL min(-1) using diode array detector. Validation procedures showed that the method was specific, accurate and precise. The amount of lycorine in S. sicula samples ranged between 0.10-0.53%, whereas in those of S. lutea and P. maritimum, the range was 0.19-0.40% and 0.05-0.14%, respectively.Ege UniversityEge University [04-ECZ-023]This study was financially supported by the Ege University Research Fund (Project No: 04-ECZ-023)

Phytochemical investigation of Galanthus woronowii

WOS: 000329421000045Ege University Research FundEge University [09/ECZ/021]; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK); EBILTEMEge UniversityThis work was partially supported Ege University Research Fund (09/ECZ/021), TUBITAK and EBILTEM