Production of IFN-β during Listeria monocytogenes Infection Is Restricted to Monocyte/Macrophage Lineage

The family of type I interferons (IFN), which consists of several IFN-α and one IFN-β, are produced not only after stimulation by viruses, but also after infection with non-viral pathogens. In the course of bacterial infections, these cytokines could be beneficial or detrimental. IFN-β is the primary member of type I IFN that initiates a cascade of IFN-α production. Here we addressed the question which cells are responsible for IFN-β expression after infection with the intracellular pathogen Listeria monocytogenes by using a genetic approach. By means of newly established reporter mice, maximum of IFN-β expression was observed at 24 hours post infection in spleen and, surprisingly, 48 hours post infection in colonized cervical and inguinal lymph nodes. Colonization of lymph nodes was independent of the type I IFN signaling, as well as bacterial dose and strain. Using cell specific reporter function and conditional deletions we could define cells expressing LysM as the major IFN-β producers, with cells formerly defined as Tip-DCs being the highest. Neutrophilic granulocytes, dendritic cells and plasmacytoid dendritic cells did not significantly contribute to type I IFN production

ESTIMATION OF INFLUENCE OF MACROCOMPONENT COMPOSITION OF MINERAL WATERS ON ELECTRO-THERMAL ATOMIC-ABSORPTION DETERMINATION OF CADMIUM, LEAD AND COPPER

General conformities to the law of influencing of macrocomponent composition of mineral waters on direct atomic-absorption determination of cadmium, lead and copper are explored. It is shown that the increase of concentrations of components of macrocomposition of test causes appropriate change of analytical signals. Scope concentrations of salts for cadmium, lead and copper are set

Complex Estimation of Functional Changes in the Organism of Healthy Rats Which Consumed Water of the Lake Kagul as Drinking Water.

In the work results of a complex estimation of functional changes in an organism of healthy rats which consumed water of the lake of Kagul as drinking water are given. Conformity of water of the lake of Kagul to requirements of operating standard documents except for nitrogen ammonia, nitrogen nitrit and the general organic carbon is established. 3 kinds of cyanobacteriaе, in particular high levels of Aphanocapsa pulverea, which causes water "flowering" are revealed. It is shown that the use of water of the lake of Kagul as drinking, causes strengthening of activity of central nervous system in healthy rats; some increase of possibilities of organism sensitisation development and specific phagocytosis, certain displays of disbalance of hummoral component of the immune response and expansion of autoimmune processes in the organism. In the view of absence of hyginicaly significant concentration of anthropogenous pollutants, the idea that the revealed biological effects are a consequence of cyanotoxins action produced by revealed cyanobacteriaе is presented. Necessity of expansion and continuation of researches on cyanobacteriaе in the context of their revealing in water, their identifications, influences of these xenobiotics on condition of warm-blooded animals and the human is proved

DESCRIPTION OF PHYSICAL AND CHEMICAL PROPERTIES OF MUDS OF PRICHERNOMORSKI ESTUARIES

Description of physical and chemical properties of muds of prichernomorski estuaries (Shabolatsky /Budaksky/, Khadzhibeysky, Kuyalnitsky, Burnas) is presented. Their conformity to the requirements shown to muds is confirmed

DESCRIPTION of ANTHROPOGENIC CONTAMINATION of muds of PRICHERNOMORSKI ESTUARIES

Description of anthropogenic contamination of muds of prichernomorski estuaries (Shabolatsky/Budaksky/, Khadzhibeysky, Kuyalnitsky, Burnas) is presented. The necessity of systemed ecologic – hygienic monitoring of estuaries is proved

Comprehensive assessment of structural and functional changes in healthy rats that consumed as drinking water that of lake Katlabuh

Робота присвячена комплексній оцінці функціональних змін в організмі здорових щурів, що споживали в якості питної воду оз. Катлабух. Результати фізико-хімічних та санітарно-хімічних досліджень свідчать про відповідність води вимогам ДСТУ 4808:2007 до джерел 2-4 класу якості. У воді оз. Катлабух виявлено 2 види ціанобактерійMerismopedia minima та Spirulina laxissima, які викликають "цвітіння" води. Встановлено, що вживання здоровими щурами якпитної води оз. Катлабух супроводжується наступними ефектами: компенсаторною активацією перекісного окиснення ліпідів у комбінації із ослабленням системи антиоксидантного захисту; патоморфологічними змінами: дистрофічними у печінці, гіпоксичними - у головному мозку, ознаками виснаження компенсаторної активності - у селезінці. Зважаючи на відсутність гігієнічно значимих концентрацій антропогенних забруднювачів, висловлено думку, що виявлені біологічні ефекти є наслідком дії ціанотоксинів або токсичних органомінеральних комплексів. Обгрунтована доцільність розширення та продовження досліджень ціанобактерій у контекстах їх виявлення у воді, ідентифікації ціанотоксинів, впливу цих ксенобіотиків на стан теплокровних тварин та людини.The work is devoted to a comprehensive assessment of functional changes in healthy rats organism who consumed as drinking water that of the lake Katlabuh. The results of physico-chemical and sanitary-chemical investigations of the lake Katlabuh water indicate its compliance with the requirements of Ukrainian state standard (DSTU) 4808: 2007 to the sources of 2-4 class quality. 2 species of cyanobacteria Merismopedia minima and Spirulina laxissima, which cause water bloom have been found. The use of healthy rats as a drinking water that of lake Katlabuh was accompanied by compensatory activation of lipid peroxidation in combination with the weakening of the antioxidant defense system. Pathomorphological changes, e.g. degenerative liver changes, hypoxic changes in brain, signs of compensatory depletion activity in the spleen took place as well. It is suggested that the biological effects identified are a consequence of the action of eeither cyanotoxins or any toxic organomineral complexes. The expediency of expansion and continued research of cyanobacteria in the contexts of their detection in water, the identification of cyanotoxins, the impact of these xenobiotics on the state of warm-blooded animals and humans has been substantuated

Комплексная эколого-гигиеническая оценка Шаболатского (Будакского) лимана: первые достижения

В работе представлены результаты эколого-гигиенической оценки рапы и пелоидов Шаболатского (Будакского) лимана по результатам физико-химических и микробиологических исследований. Обоснована вероятность сброса бытовых и промышленных сточных вод и необходимость продолжения эколого-гигиенического мониторинга лимана.The results of ecological-and-hygienic estimation of bittern and mud of Shabolatsky (Budaksky) estuary by results of physical, chemical and microbiological research are presented in the article. A probability of the dump of household and industrial sewage water and a necessity of the continuation of ecological-and-hygienic monitoring of estuary has been proved