40 research outputs found
Decrease of apoptosis rate in patients with renal transplantation treated with mycophenolate mofetil
We conclude that treatment with MMF of kidney transplant patients does not affect the proliferative rate of cells of the allograft, but decreases the number of apoptotic cells in tubular epithelium
Nuclear factor-kappa B in the liver of patients with chronic hepatitis C: decreased RelA expression is associated with enhanced fibrosis progression
The mechanisms of liver damage in chronic hepatitis C virus (HCV) infection are poorly understood. The transcription factor, nuclear factor-kappa B (NF-kappa B), regulates the expression of genes involved in apoptosis, inflammation, and antiviral response. It plays a protective role in several forms of liver damage. In this study, we analyzed NF-kappa B by gel mobility shift assay and immunohistochemistry in liver biopsies from HCV-infected patients, and we have determined the hepatic levels of the components of the NF-kappa B system by semiquantitative polymerase chain reaction (PCR). We found that NF-kappa B was activated in the liver of patients with chronic hepatitis C. Neither NF-kappa B activity nor the RNA levels of NF-kappa B subunits showed correlation with liver inflammatory activity, viral load, or HCV genotype. By contrast, hepatic mRNA values of RelA, the main element of active NF-kappa B, correlated inversely with apoptosis (r = -.68; P <.05) and with the rate of fibrosis progression (r = -.51; P <.04). In intermediate/rapid fibrosers, RelA mRNA levels were significantly decreased as compared with slow fibrosers (P <.003) and with normal livers (P <.03). In conclusion, we found that NF-kappa B is activated in chronic HCV-infected livers, and that the expression of RelA is inversely correlated with liver cell apoptosis and with the rate of fibrosis progression. Our data thus suggest that RelA expression may protect against liver fibrosis and hepatocellular damage
Idiopathic adulthood ductopenia: long-term follow-up after liver transplantation
In 1988, Ludwig et al proposed the term idiopathic adulthood ductopenia (IAD) for the condition of chronic cholestatic liver disease associated with loss of intrahepatic bile ducts of unknown etiology with clinical onset in adulthood (1).
In recent years, several cases of idiopathic biliary ductopenia in adulthood have been described. This disease is severe in most cases and can progress to cirrhosis. For progressive IAD, orthotopic liver transplantation (OLT) has been the only successful treatment. There are only a few cases reported of OLT for IAD and no information is available about the prognosis of these patients in the long term after OLT. In the present report, we describe the long-term follow-up after OLT in one patient fulfilling IAD criteria
Angiomiolipoma epitelioide hepático
Abstract: We describe the case of a hepatic epithelioid angiomyolipoma in a 50-year-old woman with epigastric pain and well-circumscribed 47 mm hepatic mass detected by ultrasonography. Angiomyolipoma is a rare benign mesenchymal tumor of the liver, composed of variable amounts of smooth muscle cells, abnormal blood vessels and adipose tissue. Preoperative diagnosis is difficult. Immunoreactivity with HMB-45 antibody helps to distinguish this tumor from other benign and malignant tumors of the liver
Secuelas tras inyección ilegal de silicona líquida como técnica de aumento mamario: presentación de 2 casos
The use of liquid silicone for breast augmentation
was widespread in the 1960s but was abandoned at
the end of the decade due to numerous studies describing
the development of a large number of local
complications, as well as remote migration of small
amounts of silicone. The use of liquid silicone also leads
to enormous difficulty in the early diagnosis of
breast cancer; these patients are precluded from routine
screening programs and must undergo exhaustive
periodic examinations. Magnetic resonance imaging
has become the most effective test for the early
detection of breast cancer in these patients. Indications
for subcutaneous mastectomy are the presence
of local complications, suspicion of a malignant lesion,
or the patient’s desire to prevent both these potential
problems
Molecular features in a biphenotypic small cell sarcoma with neuroectodermal and muscle differentiation
We report a case of a 13-year-old girl with soft tissue sarcoma of the hand,
which showed muscle and neuroectodermal immunophenotypes. Molecular studies were
performed on RNA collected from fine-needle aspiration (FNA) cytology and
peripheral blood samples by nested reverse transcriptase-polymerase chain
reaction (RT-PCR) and Southern blot analysis. This biphenotypic tumor showed
simultaneous expression of EWS-FLI1 and PAX3-FKHR transcripts, specific of Ewing
family tumors and alveolar rhabdomyosarcoma, respectively. Although childhood
sarcomas with simultaneous muscle and neural differentiation have been described
to have EWS-FLI1 transcripts, there are no reports of tumors with both
transcripts. Cytological specimens are a good source of RNA for molecular
studie
Lymphoma of the gastric stump: report of a case
We report a case of primary lymphoma on a previously resected stomach in a 62-year-old man. The patient was treated 22 years earlier with a partial gastrectomy and Billroth II reconstruction for a benign gastric ulcer. The rarity of this entity and its possible relationship with pseudolymphoma or lymphoid nodular hyperplasia is discussed, and the literature is reviewed
Gastroduodenal injury after radioembolization of hepatic tumors
Radioembolization is a new tool for the treatment of hepatic tumors
that consists in the injection of biocompatible microspheres carrying
radioisotopes into the hepatic artery or its branches. METHODS: We have performed
radioembolization in 78 patients with hepatic tumors using resin-based
microspheres loaded with yttrium-90. All patients were previously evaluated to
minimize the risk of hazardous irradiation to nontarget organs and to obtain the
data needed for dose calculation. RESULTS: We report a complication found in
three cases (3.8%) that consists of abdominal pain resulting from gastroduodenal
lesions and that had a chronic, insidious course. Microscopically, microspheres
were detected in the specimens obtained from all affected gastric areas. Since
these gastroduodenal lesions do not appear when nonradiating microspheres are
injected in animals, lesions are likely to be due to radiation and not to an
ischemic effect of vascular occlusion by spheres. CONCLUSIONS: We believe that a
pretreatment evaluation that includes a more thorough scrutiny of the hepatic
vascularization in search of small collaterals connecting to the gastroduodenal
tract can help prevent this awkward complicatio
Leukotriene C4 detection as an early graft function marker in liver transplantation
Leukotrienes are a group of compounds belonging to the eicosanoid family that are
formed from the metabolism of arachidonic acid by means of 5-lipoxigenase.
Leukotriene C4 (LTC4) has a pronounced proinflammatory character and is formed by
combining leukotriene A4 with glutation. This step is catalyzed mainly by the
isoenzyme 4-4 of the hepatic glutation transferases, although other enzymes may
participate in its formation. The liver plays a decisive part in the formation of this compound despite the fact that it can be synthesized along other cellular lines. In
orthotopic liver transplant (OLT), the evaluation of the early functioning of the graft is, in many cases, complex. The difficulty of evaluation lies in the absence of specific markers to indicate when the transplanted organ will prove viable notwithstanding
the damage resulting from preservation, and when these lesions are irreversible.
The aim of this study is to determine whether there is a relationship between the
ability to synthesize LTC4 immediately after OLT and the early functioning of the graft