Abstract P-45: Structure of the Bacteriophage AR9 Bacillus Subtilis Chaperonin According to Cryo-Electron Microscopy

Background: Chaperonins are a family of molecular chaperones Hsp60 (heat shock proteins 60). GroEL is a bacterial chaperonin. It ensures the correct folding of proteins, using the energy of ATP hydrolysis. Three-dimensional reconstructions of its predicted orthologs were obtained and biochemically characterized in free and nucleotide-bound states for bacteriophages EL Pseudomonas aeruginosa, OBP Pseudomonas fluorescens (Kurochkina, L. P. et al., Journal of virology, 2012; Semenyuk, P. I. et al., Biochemical Journal, 2016; Stanishneva-Konovalova, T. B. et al., Journal of Structural Biology, 2020). Physicochemical studies were carried out for the bacteriophage AR9 Bacillus Subtilis and confirmed that the protein has chaperone activity and does not require co-chaperonin to function (Semenyuk P. I. et al., International Journal of Biological Macromolecules, 2020). Methods: The recombinant chaperonin of the B. subtilis bacterial phage AR9 (gp228) was isolated and purified in a free state and vitrified in Vitrobot Mark IV. Data were collected using a Titan Krios cryo-TEM and processed in Warp, RELION and cryoSPARC software. Results: The final structures of the chaperonin were reconstructed with a C1 and C7 symmetry at the resolution of 4.5 Å and 4.0 Å respectively. Significant heterogeneity of the apical domains was addressed further using 3D classification and symmetry expansion in RELION resulting in a set of classes reflecting the conformational transition of the subunits between different states. At least four different conformational states of the subunit were clearly resolved. Conclusion: Gp228 structure show similarities between bacteriophage chaperonin and also bacterial chaperonin GroEL. It is formed by a single ring consisting of seven identical subunits, each has three domains: equatorial, intermediate, and apical. The subunits of the apo-form chaperonin Gp228 exhibit significant conformational flexibility in the apical and intermediate domains

Localized high-intensity light structures during multiple filamentation of Ti:sapphire-laser femtosecond pulses along an air path

The results of experimental studies of the transverse structure of a laser beam after multiple filamentation are presented. A ring structure of radiation is formed around individual filaments in a beam cross section inside the multiple filamentation domain, and at a dozen meters from it a common ring structure starts forming surrounding postfilamentation light channels (PFC). It is shown that the spectra of the PFC, rings, and beam are significantly different. The ring spectrum broadens asymmetrically relative to the carrier wavelength and is mainly concentrated in the short wavelength region. The PFC spectrum has a significant and more symmetrical broadening and covers the range 630–1000 nm

Filamentation of collimated Ti:sapphire-laser pulses in water

The results of experimental studies of the spatial characteristics of multiple filamentation terawatt femtosecond Ti:Salaser in water are presented. With an increase in initial power laser pulses increases the number of filaments, the length of the field is increased filamentation and reducing the length of the filaments have been shown. The distribution of the filaments in the longitudinal direction of the field of multiple filamentation has a maximum cross-sectional filament is shifted from the center to the periphery of the beam at the end region of filamentation. The minimum diameter of the beam on the track corresponds to the position of the maximum number of filaments. After the point of maximum impulse essentially loses energy in the initial direction of propagation. Upon reaching the pulse power 2 104 Pcr of multiple filamentation area is formed of a hollow cone, the apex directed to the radiation source

Multiple filamentation Ti:Sapphire-laser pulses in water

The results of experimental studies of the spatial characteristics of multiple filamentation terawatt femtosecond Ti:Salaser in water are presented. With an increase in initial power laser pulses increases the number of filaments, the length of the field is increased filamentation and reducing the length of the filaments have been shown. The distribution of the filaments in the longitudinal direction of the field of multiple filamentation has a maximum cross-sectional filament is shifted from the center to the periphery of the beam at the end region of filamentation. The minimum diameter of the beam on the track corresponds to the position of the maximum number of filaments. After the point of maximum impulse essentially loses energy in the initial direction of propagation. Upon reaching the pulse power 2 104 Pcr of multiple filamentation area is formed of a hollow cone, the apex directed to the radiation source

Filamentation of focused and collimated laser beams in liquids

Experimental results of investigations into the transformation of the spectral and spatial characteristics of femtosecond collimated and focused Ti:Sapphire-laser beams with wavelengths of 800 and 400 nm upon filamentation in continuous liquid media are presented. It is shown that broadening of the laser pulse spectrum due to phase self-modulation in the medium with a cubic nonlinearity depends on the pulse power and beam diameter. Dependences of the number of filaments, width of laser radiation spectrum, nonlinear focusing distance, and diameter of the filamentation region on the laser pulse power are measured. The existence of a relative power interval in which the explosive growth of the number of filaments occurs, is established. © (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only

Multiple filamentation of laser pulses in the glass

Results are presented of experiments on investigation of the spatial characteristics of multi-filamentation region of giga- and terawatt pulses of a Ti:sapphire laser in a glass. Dependences are obtained of the coordinate of the beginning of filamentation region, number of filaments, their distribution along the laser beam axis, and length of filaments on the pulse power. It is shown that with increasing radiation power, the number of filaments in the multi-filamentation region decreases, whereas the filament diameter has a quasiconstant value for all powers realized in the experiments. It is shown that as a certain power of the laser pulse with Gauss energy density distribution is reached, the filamentation region acquires the shape of a hollow cone with apex directed toward the radiation source

Implementation of the Multicomponent Algorithm of the Interdisciplinary Teaching Modules into Liberal Education of the University Students

The importance of the problem presented in the article is determined by the complex of educational strategies, goals and objectives of liberal education of the student - a tomorrow's professional in modern production who shows a new level of cultural identity the main characteristics of which are: awareness of the importance of liberal education in achieving life-plan, self-identification in society and at work; aspiration to make a conscious and decent choice of human values; formedness of consciousness and behavior, readiness to intercultural communication as a combination of competencies of productive interaction with people in the multicultural world. The purpose of the article is to scientifically and methodically underpin the efficiency of the multicomponent algorithm of the interdisciplinary teaching modules in liberal education of the university students. The paper submits the innovative approaches of self-transformation and self-identification of the university students in liberal education in the process of employing a multicomponent algorithm (the target, didactic, organizational - methodological, content-related and procedural components) of the interdisciplinary teaching modules. The article submissions are applicable for the teachers of the Humanities, academic registrars at the social sciences departments of the universities, graduate students and young scientists and students engaged in scientific activities. DOI: 10.5901/mjss.2015.v6n2s3p19

Filamentation of collimated Ti:sapphire-laser pulses in the glass

The results of experiments to study the spatial characteristics of multiple filamentation gigawatt laser pulses in the glass are presented. It is shown that with increasing pulse power multiple filamentation region increases in length and diameter, the distribution of filaments within the region has a maximum value when the power > 105 Pcr area filamentation takes the form of a hollow cone, the apex directed to the source of the laser radiation

Accreting on the edge: a luminosity-dependent cyclotron line in the Be/X-ray Binary 2S 1553-542 accompanied by accretion regimes transition

Accreting X-ray pulsars (XRPs) undergo luminous X-ray outbursts during which the luminosity-dependent spectral and timing features of the neutron star's emission can be analyzed in detail, thus shedding light on the accretion regime at work. We took advantage of a monitoring campaign performed with NuSTAR, Swift/XRT, AstroSat and NICER, to follow the Be/X-ray Binary 2S 1553-542 along one of its rare outbursts and trace its spectral and timing evolution. We report the discovery of a luminosity-dependent cyclotron line energy for the first time in this source. The pulse profiles and pulsed fraction also show variability along the outburst, consistently with the interpretation that the source transitions from the sub-critical to the super-critical accretion regime, separated by a critical luminosity of L$_{crit}\approx4\times10^{37}$ erg/s.Comment: Accepted on ApJ. 11 pages, 7 figures, 3 table