Global Gene Expression Profiling of Individual Human Oocytes and Embryos Demonstrates Heterogeneity in Early Development

Early development in humans is characterised by low and variable embryonic viability, reflected in low fecundity and high rates of miscarriage, relative to other mammals. Data from assisted reproduction programmes provides additional evidence that this is largely mediated at the level of embryonic competence and is highly heterogeneous among embryos. Understanding the basis of this heterogeneity has important implications in a number of areas including: the regulation of early human development, disorders of pregnancy, assisted reproduction programmes, the long term health of children which may be programmed in early development, and the molecular basis of pluripotency in human stem cell populations. We have therefore investigated global gene expression profiles using polyAPCR amplification and microarray technology applied to individual human oocytes and 4-cell and blastocyst stage embryos. In order to explore the basis of any variability in detail, each developmental stage is replicated in triplicate. Our data show that although transcript profiles are highly stage-specific, within each stage they are relatively variable. We describe expression of a number of gene families and pathways including apoptosis, cell cycle and amino acid metabolism, which are variably expressed and may be reflective of embryonic developmental competence. Overall, our data suggest that heterogeneity in human embryo developmental competence is reflected in global transcript profiles, and that the vast majority of existing human embryo gene expression data based on pooled oocytes and embryos need to be reinterpreted

Dynamical frictional phenomena in an incommensurate two-chain model

Dynamical frictional phenomena are studied theoretically in a two-chain model with incommensurate structure. A perturbation theory with respect to the interchain interaction reveals the contributions from phonons excited in each chain to the kinetic frictional force. The validity of the theory is verified in the case of weak interaction by comparing with numerical simulation. The velocity and the interchain interaction dependences of the lattice structure are also investigated. It is shown that peculiar breaking of analyticity states appear, which is characteristic to the two-chain model. The range of the parameters in which the two-chain model is reduced to the Frenkel-Kontorova model is also discussed.Comment: RevTex, 9 pages, 7 PostScript figures, to appear in Phys. Rev.

Resonant steps and spatiotemporal dynamics in the damped dc-driven Frenkel-Kontorova chain

Kink dynamics of the damped Frenkel-Kontorova (discrete sine-Gordon) chain driven by a constant external force are investigated. Resonant steplike transitions of the average velocity occur due to the competitions between the moving kinks and their radiated phasonlike modes. A mean-field consideration is introduced to give a precise prediction of the resonant steps. Slip-stick motion and spatiotemporal dynamics on those resonant steps are discussed. Our results can be applied to studies of the fluxon dynamics of 1D Josephson-junction arrays and ladders, dislocations, tribology and other fields.Comment: 20 Plain Latex pages, 10 Eps figures, to appear in Phys. Rev.

Deception and self-awareness

This paper presents a study conducted for the Shades of Grey EPSRC research project (EP/H02302X/1), which aims to develop a suite of interventions for identifying terrorist activities. The study investigated the body movements demonstrated by participants while waiting to be interviewed, in one of two conditions: preparing to lie or preparing to tell the truth. The effect of self-awareness was also investigated, with half of the participants sitting in front of a full length mirror during the waiting period. The other half faced a blank wall. A significant interaction was found for the duration of hand/arm movements between the deception and self-awareness conditions (F=4.335, df=1;76, p<0.05). Without a mirror, participants expecting to lie spent less time moving their hands than those expecting to tell the truth; the opposite was seen in the presence of a mirror. This finding indicates a new research area worth further investigation

A mild synthesis of substituted 1,8-naphthyridines

A greener method for the synthesis of substituted 1,8-naphthyridines has been developed, which is supported by reaction metric analysis. Using 2-aminonicotinaldehyde as a starting material with a variety of carbonyl reaction partners, the Friedländer reaction can be performed with high yield using water as the reaction solvent. Divergent reactivity was seen when using acrolein, and an alternative method was developed to give access to 2-vinyl-1,8-naphthyridine in high yield, and an assessment of addition reactions to 2-vinyl-1,8-naphthyridine was performed

Levy stable distributions via associated integral transform

We present a method of generation of exact and explicit forms of one-sided, heavy-tailed Levy stable probability distributions g_{\alpha}(x), 0 \leq x < \infty, 0 < \alpha < 1. We demonstrate that the knowledge of one such a distribution g_{\alpha}(x) suffices to obtain exactly g_{\alpha^{p}}(x), p=2, 3,... Similarly, from known g_{\alpha}(x) and g_{\beta}(x), 0 < \alpha, \beta < 1, we obtain g_{\alpha \beta}(x). The method is based on the construction of the integral operator, called Levy transform, which implements the above operations. For \alpha rational, \alpha = l/k with l < k, we reproduce in this manner many of the recently obtained exact results for g_{l/k}(x). This approach can be also recast as an application of the Efros theorem for generalized Laplace convolutions. It relies solely on efficient definite integration.Comment: 12 pages, typos removed, references adde

Universal Parametric Correlations of Eigenvalues of Random Matrix Ensemble

Eigenvalue correlations of random matrix ensembles as a function of an external perturbation are investigated vis the Dyson Brownian Motion Model in the situation where the level density has a hard edge singularity. By solving a linearized hydrodynamical equation, a universal dependence of the density-density correlator on the external field is found. As an application we obtain a formula for the variance of linear statistics with the parametric dependence exhibited as a Laplace transform.Comment: 23 pages, late