Transformative Professional Development: Unraveling the Complexities of Knowledge, Practice, and Beliefs

This study investigated the impact of a comprehensive professional development project focused on inquiry-based integrated lessons to improve the quality of science instruction for elementary teachers. Eleven teachers from three Northwest school districts participated in this quasi-experimental design study. A focus of the study was to investigate the intricate relationship between four components of a professional development model: the professional development intervention, teacher practice, student outcomes, and teachers’ self-efficacy for science instruction. Five different measures were used both before and after the intervention: The Local Systemic Change Observation Protocol, a content knowledge assessment, a self-efficacy survey, a student content test, and a student science attitude survey. In an effort to triangulate data, a reflective digital journal was kept by each teacher throughout the project. Results indicate that teachers involved in the professional development intervention experienced statistically significant growth in lesson quality and self-efficacy for teaching science, thus impacting their practice and their students

The origins of pain in diverticular disease: peripheral or central?

This study was designed to identify the processes which underlie pain in symptomatic diverticular disease (SDD). Our hypothesis was that a spectrum of both peripheral and central pathologies were involved, with those that had a more peripheral problem having abdominal symptoms only while those with multiple symptoms throughout the body, having an altered central pain processing. The first study examining the brain response to cutaneous pain using functional magnetic resonance imaging (fMRI) has supported this hypothesis. Although a statistically significant difference in sensory pain threshold was not demonstrated between the groups, fMRI imaging has shown greater emotional processing during pain and reduced anticipatory inhibitory responses in the high somatising symptomatic diverticular disease (HSDD) groups. However this is not as clear cut as we had anticipated which may be due to subject selection and demonstrate a spectrum of mixed peripheral and central changes as well as those with only peripheral or central components. In the second part we performed a randomized placebo controlled study of mesalazine 3gm versus placebo. Mesalazine significantly reduced expression of many genes associated with inflammation in SDD patients. A reduction in the median number of hours of pain per week was seen. The study was not designed to allow intention to treat analysis but has shown promising results which will need to be consolidated with future large scale studies. Both these studies support a tailored approach to SDD patient treatment based on the underlying pain process which can be both central and peripheral. The Patient health questionnaire 12(PHQ12) may be one simple measure of doing this, but again needs to be confirmed with further larger studies

Does training on inhibitory tasks influence alcohol consumption and attitudes?

Response inhibition - the suppression of a prepotent or ongoing action - is an executive function central to the regulation of behaviour. Response inhibition can be assessed in the laboratory using the Go/No-go or Stop-Signal tasks which both assess the capacity to withhold an inappropriate response. In the Go/No-go task, participants are required to respond rapidly to Go stimuli but to withhold that response upon No-go stimuli. In the Stop-Signal task, participants are required to respond to Go stimuli but to withhold the response when an auditory stop signal occurs subsequent to the Go stimulus

Simultaneous Induction of Glycolysis and Oxidative Phosphorylation during Activation of Hepatic Stellate Cells Reveals Novel Mitochondrial Targets to Treat Liver Fibrosis

Upon liver injury, hepatic stellate cells (HSCs) transdifferentiate to migratory, proliferative and extracellular matrix-producing myofibroblasts (e.g., activated HSCs; aHSCs) causing liver fibrosis. HSC activation is associated with increased glycolysis and glutaminolysis. Here, we compared the contribution of glycolysis, glutaminolysis and mitochondrial oxidative phosphorylation (OXPHOS) in rat and human HSC activation. Basal levels of glycolysis (extracellular acidification rate similar to 3-fold higher) and particularly mitochondrial respiration (oxygen consumption rate similar to 5-fold higher) were significantly increased in rat aHSCs, when compared to quiescent rat HSC. This was accompanied by extensive mitochondrial fusion in rat and human aHSCs, which occurred without increasing mitochondrial DNA content and electron transport chain (ETC) components. Inhibition of glycolysis (by 2-deoxy-D-glucose) and glutaminolysis (by CB-839) did not inhibit rat aHSC proliferation, but did reduce Acta2 (encoding alpha-SMA) expression slightly. In contrast, inhibiting mitochondrial OXPHOS (by rotenone) significantly suppressed rat aHSC proliferation, as well as Col1a1 and Acta2 expression. Other than that observed for rat aHSCs, human aHSC proliferation and expression of fibrosis markers were significantly suppressed by inhibiting either glycolysis, glutaminolysis or mitochondrial OXPHOS (by metformin). Activation of HSCs is marked by simultaneous induction of glycolysis and mitochondrial metabolism, extending the possibilities to suppress hepatic fibrogenesis by interfering with HSC metabolism

A community based systems diagram of obesity causes

INTRODUCTION: Application of system thinking to the development, implementation and evaluation of childhood obesity prevention efforts represents the cutting edge of community-based prevention. We report on an approach to developing a system oriented community perspective on the causes of obesity. METHODS: Group model building sessions were conducted in a rural Australian community to address increasing childhood obesity. Stakeholders (n = 12) built a community model that progressed from connection circles to causal loop diagrams using scripts from the system dynamics literature. Participants began this work in identifying change over time in causes and effects of childhood obesity within their community. The initial causal loop diagram was then reviewed and elaborated by 50 community leaders over a full day session. RESULTS: The process created a causal loop diagram representing community perceptions of determinants and causes of obesity. The causal loop diagram can be broken down into four separate domains; social influences; fast food and junk food; participation in sport; and general physical activity. DISCUSSION: This causal loop diagram can provide the basis for community led planning of a prevention response that engages with multiple levels of existing settings and systems

Living in a 2.2 World: ERA, Capacity Building and the Topography of Australian Educational Research

Early in 2011, the Australian Association for Research in Education (AARE) and the Australian Council of Deans of Education (ACDE) established a joint working party to create a strategic plan for strengthening national research capacity in the field of Education. This proposal followed the publication of Excellence of Research in Australia (ERA) 2010 results, which revealed that the national average weighting of Australian research in Field of Research 13 (FoR 13) - Education was well below the 'world standard' rating of 3.0. Moreover, the 2010 ERA data demonstrated that we had no up-to-date picture of who is involved in educational research, what their strengths are, or how they relate to one another. As an input into strategic research capacity building in Australian educational research, this project begins the process of documenting who 'we' are as educational researchers. The research described within the report used an ecological model to address the project's overarching question, which was: What is the topography of Australian educational research

Cortical differences in diverticular disease and correlation with symptom reports

Background Recent studies have shown that the brain of patients with gastrointestinal disease differ both structurally and functionally from that of controls. Highly somatizing diverticular disease (HSDD) patients were also shown to differ from low somatizing (LSDD) patients functional-ly. This study aimed to investigate how they differed structurally. Methods Four diseases subgroups were studied in a cross-sectional design: 20 patients with asympto-matic diverticular disease (ADD), 18 LSDD, 16 HSDD, and 18 with irritable bowel syn-drome. We divided DD patients into LSDD and HSDD using a cutoff of 6 on the Patient Health Questionnaire 12 Somatic Symptom (PHQ12-SS) scale. All patients underwent a 1-mm isotropic structural brain MRI scan and were assessed for somatization, hospital anxiety, depression, and pain catastrophizing. Whole brain volumetry, cortical thickness analysis and voxel-based morphometry were carried out using Freesurfer and SPM. Key Results We observed decreases in grey matter density in the left and right dorso-lateral prefrontal cortex (dlPFC), and in the mid-cingulate and motor cortex, and increases in the left (19, 20) and right (19, 38) Brodmann Areas. The average cortical thickness differed overall across groups (P=0.002) and regionally: HSDD>ADD in the posterior cingulate cortex (P=0.03), HSDD>LSDD in the dlPFC (P=0.03) and in the ventro-lateral PFC (P<0.001). The thickness of the anterior cingulate cortex and of the mid-prefrontal cortex were also found to correlate with Pain Catastrophizing (Spearman's ρ=0.24, P=0.043 uncorrected and Spearman's ρ=0.25, P=0.03 uncorrected). Conclusion & Inferences This is the first study of structural grey matter abnormalities in diverticular disease patients. The data shows brain differences in the pain network

A lesson from the wild: The natural state of eosinophils is Ly6G hi

From Wiley via Jisc Publications RouterHistory: received 2021-07-12, rev-recd 2021-08-29, accepted 2021-09-02, pub-electronic 2021-09-15Article version: VoRPublication status: PublishedFunder: Biotechnology and Biological Sciences Research Council; Grant(s): BB/P018157/1Abstract: With a long history of promoting pathological inflammation, eosinophils are now emerging as important regulatory cells. Yet, findings from controlled laboratory experiments so far lack translation to animals, including humans, in their natural environment. In order to appreciate the breadth of eosinophil phenotype under non‐laboratory, uncontrolled conditions, we exploit a free‐living population of the model organism Mus musculus domesticus. Eosinophils were present at significantly higher proportions in the spleen and bone marrow of wild mice compared with laboratory mice. Strikingly, the majority of eosinophils of wild mice exhibited a unique Ly6Ghi phenotype seldom described in laboratory literature. Ly6G expression correlated with activation status in spleen and bone marrow, but not peritoneal exudate cells, and is therefore likely not an activation marker per se. Intermediate Ly6G expression was transiently induced in a small proportion of eosinophils from C57BL/6 laboratory mice during acute infection with the whipworm Trichuris muris, but not during low‐dose chronic infection, which better represents parasite exposure in the wild. We conclude that the natural state of the eosinophil is not adequately reflected in the standard laboratory mouse, which compromises our attempts to dissect their functional relevance. Our findings emphasize the importance of studying the immune system in its natural context – alongside more mechanistic laboratory experiments – in order to capture the entirety of immune phenotypes and functions