Noninfectious retrovirus particles drive the APOBEC3/Rfv3 dependent neutralizing antibody response.

Members of the APOBEC3 family of deoxycytidine deaminases counteract a broad range of retroviruses in vitro through an indirect mechanism that requires virion incorporation and inhibition of reverse transcription and/or hypermutation of minus strand transcripts in the next target cell. The selective advantage to the host of this indirect restriction mechanism remains unclear, but valuable insights may be gained by studying APOBEC3 function in vivo. Apobec3 was previously shown to encode Rfv3, a classical resistance gene that controls the recovery of mice from pathogenic Friend retrovirus (FV) infection by promoting a more potent neutralizing antibody (NAb) response. The underlying mechanism does not involve a direct effect of Apobec3 on B cell function. Here we show that while Apobec3 decreased titers of infectious virus during acute FV infection, plasma viral RNA loads were maintained, indicating substantial release of noninfectious particles in vivo. The lack of plasma virion infectivity was associated with a significant post-entry block during early reverse transcription rather than G-to-A hypermutation. The Apobec3-dependent NAb response correlated with IgG binding titers against native, but not detergent-lysed virions. These findings indicate that innate Apobec3 restriction promotes NAb responses by maintaining high concentrations of virions with native B cell epitopes, but in the context of low virion infectivity. Finally, Apobec3 restriction was found to be saturable in vivo, since increasing FV inoculum doses resulted in decreased Apobec3 inhibition. By analogy, maximizing the release of noninfectious particles by modulating APOBEC3 expression may improve humoral immunity against pathogenic human retroviral infections

The Development of the WISE (Writing to Inspire Successful Education) Writing Mentoring Program: A University-School Collaboration

Abstract This paper describes the development of a service learning writing mentoring program designed to close the achievement gap in writing proficiency for economically disadvantaged seventh grade students. Compared to writing mentoring studies found in the published literature, this program has three distinguishing components. First, it focused on economically disadvantaged middle school students. Second, it provided writing mentoring through a university-school partnership in which college students provided the intervention in collaboration with a seventh-grade teacher. Third, the program used technology to facilitate the mentoring process. Over the course of an academic year, mentors created videos with feedback on 19 writing assignments. The writing mentoring program was associated with a four-fold increase in the percentage of students who were graded as ‘proficient’ on a state standardized writing exam. These results suggest that semi-virtual, intensive writing mentoring and individualized feedback from college students can close the achievement gap and improve the quality of middle level education provided to economically disadvantaged students

The Surface of a Bose-Einstein Condensed Atomic Cloud

We investigate the structure and collective modes of a planar surface of a trapped Bose-Einstein condensed gas at zero temperature. In the long-wavelength limit we find a mode similar to the gravity wave on the surface of a fluid with the frequency $\omega$ and the wavenumber $q$ related by $\omega^2=Fq/m$. Here $F$ is the force due to the confining potential at the surface and $m$ is the particle mass. At shorter wavelengths we use a variational approach and find corrections to $\omega^2$ of order $q^4 \ln{q}$. We demonstrate the usefulness of the concept of an effective surface tension for describing both static and dynamic properties of condensed atomic clouds.Comment: 8 pages, REVTEX, submitted to Phys. Rev.

Zero-temperature phase diagram of binary boson-fermion mixtures

We calculate the phase diagram for dilute mixtures of bosons and fermions at zero temperature. The linear stability conditions are derived and related to the effective boson-induced interaction between the fermions. We show that in equilibrium there are three possibilities: a) a single uniform phase, b) a purely fermionic phase coexisting with a purely bosonic one and c) a purely fermionic phase coexisting with a mixed phase.Comment: 8 pages, revtex, 3 postscript figures; NORDITA-1999/71 C

Considering Positive Psychology Constructs of Life Satisfaction and School Connectedness When Assessing Symptoms Related to Attention-Deficit/Hyperactivity Disorder

Children and adolescents diagnosed with Attention-Deficit/Hyperactivity Disorder (ADHD) demonstrate significant difficulty with academic and behavioral functioning. This, in turn, can lead to lower educational attainment and vocational achievement, which has serious long-term consequences and costs to individuals and society (Barkley, 2002, 2006; Mannuzza, Klein, Bessler, Malloy, & LaPadula, 1993). Researchers from a positive psychology framework suggest that ADHD symptoms (i.e., inattention, hyperactivity/impulsivity) alone may not fully explain academic impairment (Diener, Scollon, & Lucas, 2004). From the standpoint of positive psychology, life satisfaction and school connectedness are important constructs that examine positive life functioning; however, they have been understudied, particularly in the area of ADHD. The current study investigated the relationship between ADHD symptoms and these positive psychological constructs. Results indicate that as ADHD symptoms increase, life satisfaction decreases; however, no relationship between ADHD symptoms and school connectedness was found. Beyond our primary analysis, we examined the relationship between gender and these variables. Results suggest that gender significantly moderates the relationship between ADHD and life satisfaction, with life satisfaction ratings decreasing for males as ADHD symptoms increase, yet remaining stable for females. ADHD symptoms did not significantly predict changes in school connectedness

Reasoning with comparative moral judgements: an argument for Moral Bayesianism

The paper discusses the notion of reasoning with comparative moral judgements (i.e judgements of the form “act a is morally superior to act b”) from the point of view of several meta-ethical positions. Using a simple formal result, it is argued that only a version of moral cognitivism that is committed to the claim that moral beliefs come in degrees can give a normatively plausible account of such reasoning. Some implications of accepting such a version of moral cognitivism are discussed

Towards a microscopic theory of toroidal moments in bulk periodic crystals

We present a theoretical analysis of magnetic toroidal moments in periodic systems, in the limit in which the toroidal moments are caused by a time and space reversal symmetry breaking arrangement of localized magnetic dipole moments. We summarize the basic definitions for finite systems and address the question of how to generalize these definitions to the bulk periodic case. We define the toroidization as the toroidal moment per unit cell volume, and we show that periodic boundary conditions lead to a multivaluedness of the toroidization, which suggests that only differences in toroidization are meaningful observable quantities. Our analysis bears strong analogy to the modern theory of electric polarization in bulk periodic systems, but we also point out some important differences between the two cases. We then discuss the instructive example of a one-dimensional chain of magnetic moments, and we show how to properly calculate changes of the toroidization for this system. Finally, we evaluate and discuss the toroidization (in the local dipole limit) of four important example materials: BaNiF_4, LiCoPO_4, GaFeO_3, and BiFeO_3.Comment: replaced with final (published) version, which includes some changes in the text to improve the clarity of presentatio

Vortices in Bose-Einstein-Condensed Atomic Clouds

The properties of vortex states in a Bose-Einstein condensed cloud of atoms are considered at zero temperature. Using both analytical and numerical methods we solve the time-dependent Gross-Pitaevskii equation for the case when a cloud of atoms containing a vortex is released from a trap. In two dimensions we find the simple result that the time dependence of the cloud radius is given by $(1+\omega^2t^2)^{1/2}$, where $\omega$ is the trap frequency. We calculate and compare the expansion of the vortex core and the cloud radius for different numbers of particles and interaction strengths, in both two and three dimensions, and discuss the circumstances under which vortex states may be observed experimentally.Comment: Revtex, 11 pages including 5 eps figures, submitted to Phys. Rev. A; new reference added, remark added in Sec. IIIB, axis label added in Fig.

Change in Metabolic Syndrome and Cardiorespiratory Fitness Following Exercise Training – The Ball State Adult Fitness Longitudinal Lifestyle Study (BALL ST)

This observational cohort study indicates an inverse relationship between the change in CRF and the change in MetS severity following exercise training. These results suggest that participation in a community-based exercise program yields significant improvements in CRF, MetS risk factors, the prevalence of the binary MetS, and the MetS severity score. Improvement in CRF through exercise training should be a primary prevention strategy for MetS

Rapid fixation of non-native alleles revealed by genome-wide SNP analysis of hybrid tiger salamanders

<p>Abstract</p> <p>Background</p> <p>Hybrid zones represent valuable opportunities to observe evolution in systems that are unusually dynamic and where the potential for the origin of novelty and rapid adaptation co-occur with the potential for dysfunction. Recently initiated hybrid zones are particularly exciting evolutionary experiments because ongoing natural selection on novel genetic combinations can be studied in ecological time. Moreover, when hybrid zones involve native and introduced species, complex genetic patterns present important challenges for conservation policy. To assess variation of admixture dynamics, we scored a large panel of markers in five wild hybrid populations formed when Barred Tiger Salamanders were introduced into the range of California Tiger Salamanders.</p> <p>Results</p> <p>At three of 64 markers, introduced alleles have largely displaced native alleles within the hybrid populations. Another marker (<it>GNAT1</it>) showed consistent heterozygote deficits in the wild, and this marker was associated with embryonic mortality in laboratory F2's. Other deviations from equilibrium expectations were idiosyncratic among breeding ponds, consistent with highly stochastic demographic effects.</p> <p>Conclusion</p> <p>While most markers retain native and introduced alleles in expected proportions, strong selection appears to be eliminating native alleles at a smaller set of loci. Such rapid fixation of alleles is detectable only in recently formed hybrid zones, though it might be representative of dynamics that frequently occur in nature. These results underscore the variable and mosaic nature of hybrid genomes and illustrate the potency of recombination and selection in promoting variable, and often unpredictable genetic outcomes. Introgression of a few, strongly selected introduced alleles should not necessarily affect the conservation status of California Tiger Salamanders, but suggests that genetically pure populations of this endangered species will be difficult to maintain.</p