1,431 research outputs found
Electronic structure of small GaAs clusters
The electronic structure of small Ga_xAs_y clusters (x+y≤10) are calculated using the local density method. The calculation shows that even‐numbered clusters tend to be singlets, as opposed to odd‐numbered clusters which are open shell systems. This is in agreement with the experimental observations of even/odd alternations of the electron affinity and ionization potential. In the larger clusters, the atoms prefer an alternating bond arrangement; charge transfers are observed from Ga sites to As sites. This observation is also in agreement with recent chemisorption studies of ammonia on GaAs clusters. The close agreement between theoretical calculations and experimental results, together with the rich variation of electronic properties of GaAs clusters with composition makes GaAs clusters an ideal prototype system for the study of how electronic structure influences chemical reactivity
Ki67 expression levels are a better marker of reduced melanoma growth following MEK inhibitor treatment than phospho-ERK levels
The loss of tumour phospho-extracellular responsive kinase (pERK) positivity is the major treatment biomarker for mitogen-activated protein kinase/extracellular responsive kinase (MEK) inhibitors. Here, we demonstrate that there is a poor correlation between pERK inhibition and the anti-proliferative effects of MEK inhibitors in melanoma cells. We suggest that Ki67 is a better biomarker for future clinical studies
CD24 staining of mouse mammary gland cells defines luminal epithelial, myoepithelial/basal and non-epithelial cells
INTRODUCTION: Breast cancer is thought to arise in mammary epithelial stem cells. There is, therefore, a large amount of interest in identifying these cells. The breast is a complex tissue consisting of two epithelial layers (an outer myoepithelial/basal layer and an inner luminal epithelial layer) as well as a large non-epithelial component (fibroblasts, endothelial cells, lymphocytes, adipocytes, neurons and myocytes). The definitive identification of a mammary epithelial stem cell population is critically dependent on its purity. To date, this has been hampered by the lack of suitable markers to separate out the two epithelial layers, and to remove contaminating non-epithelial cells. METHODS: Mouse mammary glands were dissociated and stained with CD24. Cells were sorted into separate populations based on CD24 expression and assessed for luminal epithelial and myoepithelial/basal markers by direct fluorescent microscopy and real time PCR. The stem/progenitor potential of these cell populations was assessed in vivo by cleared mammary fat pad transplantation. RESULTS: Three populations of CD24 expressing cells were identified: CD24(Negative), CD24(Low )and CD24(High). Staining of these cells with cytokeratin markers revealed that these populations correspond to non-epithelial, myoepithelial/basal and luminal epithelial cells, respectively. Cell identities were confirmed by quantitative PCR. Cleared mammary fat pad transplantation of these cell populations revealed that extensive mammary fat pad repopulation capacity segregates with the CD24(Low )cells, whilst CD24(High )cells have limited repopulation capacity. CONCLUSION: Differential staining of mammary epithelial cells for CD24 can be used to simultaneously isolate pure populations of non-epithelial, myoepithelial/basal and luminal epithelial cells. Furthermore, mammary fat pad repopulation capacity is enriched in the CD24(Low )population. As separation is achieved using a single marker, it will be possible to incorporate additional markers to further subdivide these populations. This will considerably facilitate the further analysis of mammary epithelial subpopulations, whilst ensuring high purity, which is key for understanding mammary epithelial stem cells in normal tissue biology and carcinogenesis
Long-Term Clustering, Scaling, and Universality in the Temporal Occurrence of Earthquakes
Scaling analysis reveals striking regularities in earthquake occurrence. The
time between any one earthquake and that following it is random, but it is
described by the same universal-probability distribution for any spatial region
and magnitude range considered. When time is expressed in rescaled units, set
by the averaged seismic activity, the self-similar nature of the process
becomes apparent. The form of the probability distribution reveals that
earthquakes tend to cluster in time, beyond the duration of aftershock
sequences. Furthermore, if aftershock sequences are analysed in an analogous
way, yet taking into account the fact that seismic activity is not constant but
decays in time, the same universal distribution is found for the rescaled time
between events.Comment: short paper, only 2 figure
Loop-mediated isothermal amplification (LAMP) for the rapid detection of Mycoplasma genitalium
Mycoplasma genitalium is a sexually transmissible, pathogenic bacterium and a significant cause of nongonococcal urethritis in both men and women. Due to the difficulty of the culture of M. genitalium from clinical samples, the laboratory diagnosis of M. genitalium infection is almost exclusively carried out using nucleic acid amplification tests. Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification technology, utilising a set of 4 primers specific to 6 distinct regions of the target DNA sequence, in order to amplify target DNA in a highly specific and rapid manner. A LAMP assay was designed to the pdhD gene of M. genitalium, and the limit of detection of the assay was determined as 10. fg of M. genitalium genomic DNA, equating to ~16 copies of the M. genitalium genome, which was equally sensitive as a gold standard 16S rRNA polymerase chain reaction assay. © 2015 Elsevier Inc
Convection, Thermal Bifurcation, and the Colors of A stars
Broad-band ultraviolet photometry from the TD-1 satellite and low dispersion
spectra from the short wavelength camera of IUE have been used to investigate a
long-standing proposal of Bohm-Vitense that the normal main sequence A- and
early-F stars may divide into two different temperature sequences: (1) a high
temperature branch (and plateau) comprised of slowly rotating convective stars,
and (2) a low temperature branch populated by rapidly rotating radiative stars.
We find no evidence from either dataset to support such a claim, or to confirm
the existence of an "A-star gap" in the B-V color range 0.22 <= B-V <= 0.28 due
to the sudden onset of convection. We do observe, nonetheless, a large scatter
in the 1800--2000 A colors of the A-F stars, which amounts to ~0.65 mags at a
given B-V color index. The scatter is not caused by interstellar or
circumstellar reddening. A convincing case can also be made against binarity
and intrinsic variability due to pulsations of delta Sct origin. We find no
correlation with established chromospheric and coronal proxies of convection,
and thus no demonstrable link to the possible onset of convection among the A-F
stars. The scatter is not instrumental. Approximately 0.4 mags of the scatter
is shown to arise from individual differences in surface gravity as well as a
moderate spread (factor of ~3) in heavy metal abundance and UV line blanketing.
A dispersion of ~0.25 mags remains, which has no clear and obvious explanation.
The most likely cause, we believe, is a residual imprecision in our correction
for the spread in metal abundances. However, the existing data do not rule out
possible contributions from intrinsic stellar variability or from differential
UV line blanketing effects owing to a dispersion in microturbulent velocity.Comment: 40 pages, 14 figures, 1 table, AAS LaTex, to appear in The
Astrophysical Journa
Evidence for a Long-Standing Top-Heavy IMF in the Central Parsec of the Galaxy
We classify 329 late-type giants within 1 parsec of Sgr A*, using the
adaptive optics integral field spectrometer SINFONI on the VLT. These
observations represent the deepest spectroscopic data set so far obtained for
the Galactic Center, reaching a 50% completeness threshold at the approximate
magnitude of the helium-burning red clump (Ks ~ 15.5 mag.). Combining our
spectroscopic results with NaCo H and Ks photometry, we construct an observed
Hertzsprung-Russell diagram, which we quantitatively compare to theoretical
distributions of various star formation histories of the inner Galaxy, using a
chi-squared analysis. Our best-fit model corresponds to continuous star
formation over the last 12 Gyr with a top-heavy initial mass function (IMF).
The similarity of this IMF to the IMF observed for the most recent epoch of
star formation is intriguing and perhaps suggests a connection between recent
star formation and the stars formed throughout the history of the Galactic
Center.Comment: 18 pages, 10 figures, Accepted to ApJ: 15 July 200
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