Zelinski Lab: Macaque Ovary Tissue Histology

A collection of ovary histology slides and associated meta-data for non-human primates

Zelinski Lab: Japanese Macaque Ovary Histology

A collection of ovary histology slides and associated metadata for Japanese Macaque primates

Training Materials and Protocols

This component contains training materials (e.g., videos, slides, documents) for the MOTHER project

MOTHER: Multispecies Ovary Tissue Histology Electronic Repository

The Multispecies Ovary Tissue Histology Electronic Repository (MOTHER) facilitates comparative studies of female reproductive strategies, enabling the development of computational models to test hypotheses related to ovarian development and female reproduction, and serving as an educational resource thereby reducing the use of animals in research

Zelinski Lab: Cynomolgus Macaque Ovary Histology

A collection of ovary histology slides and associated metadata for Cynomolgus Macaque primates

Zelinski Lab: Rhesus Macaque Ovary Histology

A collection of ovary histology slides and associated metadata for Rhesus Macaque primates

A simple automated method for continuous fieldwise measurement of microvascular hemodynamics

Microvascular perfusion dynamics are vital to physiological function and are frequently dysregulated in injury and disease. Typically studies measure microvascular flow in a few selected vascular segments over limited time, failing to capture spatial and temporal variability. To quantify microvascular flow in a more complete and unbiased way we developed STAFF (Spatial Temporal Analysis of Fieldwise Flow), a macro for FIJI open-source image analysis software. Using high-speed microvascular flow movies, STAFF generates kymographs for every time interval for every vascular segment, calculates flow velocities from red blood cell shadow angles, and outputs the data as color-coded velocity map movies and spreadsheets. In untreated mice, analyses demonstrated profound variation even between adjacent sinusoids over seconds. In acetaminophen-treated mice we detected flow reduction localized to pericentral regions. STAFF is a powerful new tool capable of providing novel insights by enabling measurement of the complex spatiotemporal dynamics of microvascular flow