3 research outputs found
Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering
The present work focuses on the development of novel multicomponent organicâinorganic hydrogel composites for bone tissue engineering. For the first time, combination of the organic components commonly used in food industry, namely whey protein isolate (WPI) and gelatin from bovine skin, as well as inorganic material commonly used as a major component of hydraulic bone cements, namely αâTCP in various concentrations (0â70 wt.%) was proposed. The results showed that αâTCP underwent incomplete transformation to calciumâdeficient hydroxyapatite (CDHA) during preparation process of the hydrogels. Microcomputer tomography showed inhomogeneous distribution of the calcium phosphate (CaP) phase in the resulting composites. Nevertheless, hydrogels containing 30â70 wt.% αâTCP showed significantly improved mechanical properties. The values of Young's modulus and the stresses corresponding to compression of a sample by 50% increased almost linearly with increasing concentration of ceramic phase. Incomplete transformation of αâTCP to CDHA during preparation process of composites provides them high reactivity in simulated body fluid during 14âday incubation. Preliminary in vitro studies revealed that the WPI/gelatin/CaP composite hydrogels support the adhesion, spreading, and proliferation of human osteoblastâlike MGâ63 cells. The WPI/gelatin/CaP composite hydrogels obtained in this work showed great potential for the use in bone tissue engineering and regenerative medicine applications
In vivo application of foam titanium implants with magnesium-doped octacalcium phosphate and hydroxyapatite thin films deposited by PLD method
Results of investigations into integration of foam titanium implants (FTI), with rabbit bone are presented. Three groups of implants were examined: clean titanium implants produced by selective laser melting, FTI coated with a thin film of octacalcium phosphate (OCP), doped with 0.6 weight percent of Mg, deposited by pulsed laser deposition (PLD), and FTI with hydroxyapatite (HA) doped with 0.6 wt.% of Mg. Analysis of histopathology of implants' integration with bone were performed by staining using the triple chromatographic method of Masson - Goldner, pla- nimetric analysis using the Kruskal - Wallis test, an ANOVA test and microtomography. The best results were obtained for implants coated with HA, followed by those coated with OCP. Implants from these two groups showed superior integration with bone than uncoated titanium implants