Estudo do potencial desmineralizante de bebidas energéticas sobre a hidroxiapatita

Orientador: Prof.Dr. José Vitor Nogara Borges de MenezesOrientador: Prof.Dr. José Miguel Amenábar CéspedesDissertação (mestrado) - Universidade Federal do Paraná, Setor de Ciências da Saúde, Programa de Pós-Graduação em Odontologia. Defesa: Curitiba, 31/03/2014Inclui referênciasÁrea de concentraçãoResumo: A desmineralização dental ocasionada por ácidos pode originar tanto a erosão quanto a cárie. Erosão é um processo patológico, progressivo e localizado, levando à perda de tecido dental duro, o qual é quimicamente removido da superfície dentária por ácidos e/ou quelação sem envolvimento bacteriano. A fonte extrínseca mais importante de exposição ácida é a dieta, tornando-se fundamental o conhecimento de agentes potencialmente erosivos tais como as bebidas energéticas. O objetivo deste estudo foi avaliar o potencial desmineralizante de algumas bebidas energéticas sobre a hidroxiapatita (HAP). Com base nas características de acidez das bebidas energéticas, um estudo in vitro foi desenvolvido. Nove diferentes marcas de energéticos (Burn®, Red Bull®, Fusion®, Wild Dragon®, Fire Bird®, Insano®, Flying Horse®, Monster® e Psycho Drink®) comercializadas no Brasil, foram utilizadas para avaliar seu possível dano à HAP. Para isso, análises de pH e acidez titulável dos energéticos e concentrações de cálcio (Ca) e fósforo (P) da HAP após sua exposição à essas bebidas foram realizadas. Os tempos de exposição foram de 1, 3, 5, 7, 10 e 15 minutos. Os resultados foram analisados estatisticamente por teste de ANOVA, pós-teste de Tukey e teste de correlação de Pearson (p?0,05). Com base nos valores de perda de Ca e P da HAP, o energético que apresentou a maior perda de Ca foi o Fire Bird® no tempo de 15 minutos e a menor foi o Red Bull® no tempo de 10 minutos. A bebida energética que apresentou a maior perda de P foi o Fusion® no tempo de 7 minutos, e a menor foi o Wild Dragon®, também no tempo de 7 minutos. Todas as bebidas energéticas, independente do seu pH e acidez titulável, ocasionaram desmineralização da HAP.Abstract: The tooth demineralization caused by acids may lead to erosion as well as decay. Erosion is a localized pathological progressive process, leading to loss of dental hard tissue, chemically removed from the tooth surface by acid and/or chelation without bacterial involvement. The most important extrinsic source of acid exposure is diet, so awareness of potential erosive agents, such as energy drinks, is important. The study aimed at investigating the demineralizing potential of some energy drinks on hydroxyapatite (HAP). Based on the acidity characteristics of energy drinks, an in vitro experimental study was developed. Nine different brands of energy drinks( Burn®, Red Bull®, Fusion®, Wild Dragon®, Fire Bird®, Insano®, Flying Horse®, Monster® and Psycho Drink®) traded in Brazil were used to evaluate their potential damage in HAP. In order to address that question, analyses of pH, titratable acidity, and concentrations of Ca and P of HAP after exposure to these drinks were conducted. The exposure times were 1, 3, 5, 7, 10, and 15 minutes. The results were statistically analysed by ANOVA test, Tukey post test and Pearson?s correlation test (p?0,05). Based on values of Ca and P losses on HAP, the energy drink that showed the largest loss of Ca was Fire Bird® in 15 minutes observation and the lowest was Red Bull® in 10 minutes observation. The energy drink with the highest P loss was Fusion® in 7 minutes observation and the lowest was Wild Dragon® also in 7 minutes observation. All energy drinks, regardless of their pH and titratable acidity, caused demineralization of HAP

Dental extraction in patients with HIV/AIDS: report of two cases

Introduction and objective: The oral health of patients with HIV infection is often compromised by caries and periodontal disease. Thus, many patients need to undergo oral surgical procedures. Case report: This article describes two cases of patients with HIV infection who had undergone exodontia due to prosthetic indications. Both patients had been hospitalized for treatment of respiratory complications from HIV infection and were referred for dental treatment. In the first case, the adult patient had generally good oral health. However, the treatment plan for the installation of a removable prosthesis required the removal of tooth 38 since it was fairly inclined to the mesial. The second patient had poor oral conditions due to advanced periodontal disease. Thus, all upper arch teeth were removed in a single session followed by the installationof an immediate total prosthesis. No postoperative complications were recorded and the healing process occurred without incident for both patients. Dental treatment of patients with asymptomatic HIV infection does not differ from that performed for any other patient in practice. Nevertheless, patients in advanced stages of disease may require special treatment and an individual treatment plan must be developed for even routine procedures. Conclusion: The dental careof these individuals often requires more rigorous clinical follow-up for maintaining oral health. The performance of dental surgery in patients with HIV infection does not require technical modifications, but does require a complete anamnesis.Introduction and objective: The oral health of patients with HIV infection is often compromised by caries and periodontal disease. Thus, many patients need to undergo oral surgical procedures. Case report: This article describes two cases of patients with HIV infection who had undergone exodontia due to prosthetic indications. Both patients had been hospitalized for treatment of respiratory complications from HIV infection and were referred for dental treatment. In the first case, the adult patient had generally good oral health. However, the treatment plan for the installation of a removable prosthesis required the removal of tooth 38 since it was fairly inclined to the mesial. The second patient had poor oral conditions due to advanced periodontal disease. Thus, all upper arch teeth were removed in a single session followed by the installationof an immediate total prosthesis. No postoperative complications were recorded and the healing process occurred without incident for both patients. Dental treatment of patients with asymptomatic HIV infection does not differ from that performed for any other patient in practice. Nevertheless, patients in advanced stages of disease may require special treatment and an individual treatment plan must be developed for even routine procedures. Conclusion: The dental careof these individuals often requires more rigorous clinical follow-up for maintaining oral health. The performance of dental surgery in patients with HIV infection does not require technical modifications, but does require a complete anamnesis

Estudo do potencial desmineralizante de bebidas energéticas sobre a hidroxiapatita

Orientador: Prof.Dr. José Vitor Nogara Borges de MenezesOrientador: Prof.Dr. José Miguel Amenábar CéspedesDissertação (mestrado) - Universidade Federal do Paraná, Setor de Ciências da Saúde, Programa de Pós-Graduação em Odontologia. Defesa: Curitiba, 31/03/2014Inclui referênciasÁrea de concentraçãoResumo: A desmineralização dental ocasionada por ácidos pode originar tanto a erosão quanto a cárie. Erosão é um processo patológico, progressivo e localizado, levando à perda de tecido dental duro, o qual é quimicamente removido da superfície dentária por ácidos e/ou quelação sem envolvimento bacteriano. A fonte extrínseca mais importante de exposição ácida é a dieta, tornando-se fundamental o conhecimento de agentes potencialmente erosivos tais como as bebidas energéticas. O objetivo deste estudo foi avaliar o potencial desmineralizante de algumas bebidas energéticas sobre a hidroxiapatita (HAP). Com base nas características de acidez das bebidas energéticas, um estudo in vitro foi desenvolvido. Nove diferentes marcas de energéticos (Burn®, Red Bull®, Fusion®, Wild Dragon®, Fire Bird®, Insano®, Flying Horse®, Monster® e Psycho Drink®) comercializadas no Brasil, foram utilizadas para avaliar seu possível dano à HAP. Para isso, análises de pH e acidez titulável dos energéticos e concentrações de cálcio (Ca) e fósforo (P) da HAP após sua exposição à essas bebidas foram realizadas. Os tempos de exposição foram de 1, 3, 5, 7, 10 e 15 minutos. Os resultados foram analisados estatisticamente por teste de ANOVA, pós-teste de Tukey e teste de correlação de Pearson (p?0,05). Com base nos valores de perda de Ca e P da HAP, o energético que apresentou a maior perda de Ca foi o Fire Bird® no tempo de 15 minutos e a menor foi o Red Bull® no tempo de 10 minutos. A bebida energética que apresentou a maior perda de P foi o Fusion® no tempo de 7 minutos, e a menor foi o Wild Dragon®, também no tempo de 7 minutos. Todas as bebidas energéticas, independente do seu pH e acidez titulável, ocasionaram desmineralização da HAP.Abstract: The tooth demineralization caused by acids may lead to erosion as well as decay. Erosion is a localized pathological progressive process, leading to loss of dental hard tissue, chemically removed from the tooth surface by acid and/or chelation without bacterial involvement. The most important extrinsic source of acid exposure is diet, so awareness of potential erosive agents, such as energy drinks, is important. The study aimed at investigating the demineralizing potential of some energy drinks on hydroxyapatite (HAP). Based on the acidity characteristics of energy drinks, an in vitro experimental study was developed. Nine different brands of energy drinks( Burn®, Red Bull®, Fusion®, Wild Dragon®, Fire Bird®, Insano®, Flying Horse®, Monster® and Psycho Drink®) traded in Brazil were used to evaluate their potential damage in HAP. In order to address that question, analyses of pH, titratable acidity, and concentrations of Ca and P of HAP after exposure to these drinks were conducted. The exposure times were 1, 3, 5, 7, 10, and 15 minutes. The results were statistically analysed by ANOVA test, Tukey post test and Pearson?s correlation test (p?0,05). Based on values of Ca and P losses on HAP, the energy drink that showed the largest loss of Ca was Fire Bird® in 15 minutes observation and the lowest was Red Bull® in 10 minutes observation. The energy drink with the highest P loss was Fusion® in 7 minutes observation and the lowest was Wild Dragon® also in 7 minutes observation. All energy drinks, regardless of their pH and titratable acidity, caused demineralization of HAP

Efficacy of stem cells on bone consolidation of distraction osteogenesis in animal models: a systematic review

Abstract Distraction osteogenesis (DO) relies on the recruitment and proliferation of mesenchymal stem cells (MSC) to the target site, where they differentiate into osteoblasts to promote bone formation. Nevertheless, MSC recruitment appears to be slow and limits bone formation in DO defects. Thus, this systematic review aims to evaluate the ability of locally applied MSC to enhance bone formation in DO preclinical models. Databases were searched for quantitative pre-clinical controlled studies that evaluated the effect of local administration of MSC on DO bone formation. Eligible studies were identified and data regarding study characteristics, outcome measures and quality were extracted. Nine studies met the inclusion criteria. Autogenous and xenogenous MSC were used to promote DO bone formation. These included bone marrow-derived MSC, adipose tissue-derived MSC and MSC derived from human exfoliated deciduous teeth. Meta-analysis was not possible due to heterogeneities in study designs. Local MSC implantation was not associated with adverse effects. In 4 out of the 5 studies, locally delivered undifferentiated bone-marrow MSC had a positive effect on DO bone formation. Few studies evaluated the therapeutic effects of MSC from other sources. The adjunct use of biologically active molecules or forced expression of key genes involved in osteogenesis further boosted the ability of bone-marrow MSC to promote DO bone formation. While risk of bias and heterogeneity limited the strength of this systematic review, our results suggest that the use of MSC is safe and may provide beneficial effects on DO bone formation