A Partial, Age-Graded Examination of Agnew’s General Theory of Crime and Delinquency

Previous examinations of Agnew’s (2005) general theory of crime and delinquency have garnered mixed results for the theoretical construct. These previous investigations have concentrated on a singular stage of an individual’s life—with analyses focusing on either the adolescent (Muftić, Grubb, Bouffard, & Maljević, 2014; Ngo & Paternoster, 2014; Roh & Marshall, 2018; Zhang, Day, & Cao, 2012) or the adult (Cochran, 2017; Ngo, Paternoster, Cullen, & Mackenzie, 2011) time juncture—failing to empirically assess the variability hypothesis centrally proposed by Agnew. Using data from a nationally representative sample of participants—the National Longitudinal Study of Adolescent to Adult Health [n = 20,745 (Wave I), 14,738 (Wave II), 15,917 (Wave III), and 15,701 (Wave IV)]—Agnew’s general theory was applied to multiple junctures of an individual’s life (adolescence and adulthood), which provided one of the first age-graded assessments of the theoretical construct. Poisson and negative binomial regression models were constructed and analyzed, with each generated model representing a significant improvement in fit over the null/intercept-only model. Moreover, Agnew’s variability hypothesis obtained considerable empirical support, ultimately highlighting the various life domains (self and peer for adolescence; self and family for adulthood) most influential at differing time junctures. These multitude of findings led to the championing of crime prevention/behavior modification programs that specifically target the correlates of crime and delinquency that this analysis found to be most significant in predicting engagement in crime/delinquency. A few model programs argued for within are the Gang Resistance and Education Training program, the Triple P-Positive Parenting Program, and Multisystemic Therapy

Wettability and Other Surface Properties of Modified Polymers

Surface wettability is one of the crucial characteristics for determining of a material’s use in specific application. Determination of wettability is based on the measurement of the material surface contact angle. Contact angle is the main parameter that characterizes the drop shape on the solid surface and is also one of the directly measurable properties of the phase interface. In this chapter, the wettability and its related properties of pristine and modified polymer foils will be described. The wettability depends on surface roughness and chemical composition. Changes of these parameters can adjust the values of contact angle and, therefore, wettability. In the case of pristine polymer materials, their wettability is unsuitable for a wide range of applications (such as tissue engineering, printing, and coating). Polymer surfaces can easily be modified by, e.g., plasma discharge, whereas the bulk properties remain unchanged. This modification leads to oxidation of the treated layer and creation of new chemical groups that mainly contain oxygen. Immediately after plasma treatment, the values of the contact angles of the modified polymer significantly decrease. In the case of a specific polymer, the strongly hydrophilic surface is created and leads to total spreading of the water drop. Wettability is strongly dependent on time from modification

Harnessing Mechanisms of Immune Tolerance to Improve Outcomes in Solid Organ Transplantation: A Review

Survival after solid organ transplantation (SOT) is limited by chronic rejection as well as the need for lifelong immunosuppression and its associated toxicities. Several preclinical and clinical studies have tested methods designed to induce transplantation tolerance without lifelong immune suppression. The limited success of these strategies has led to the development of clinical protocols that combine SOT with other approaches, such as allogeneic hematopoietic stem cell transplantation (HSCT). HSCT prior to SOT facilitates engraftment of donor cells that can drive immune tolerance. Recent innovations in graft manipulation strategies and post-HSCT immune therapy provide further advances in promoting tolerance and improving clinical outcomes. In this review, we discuss conventional and unconventional immunological mechanisms underlying the development of immune tolerance in SOT recipients and how they can inform clinical advances. Specifically, we review the most recent mechanistic studies elucidating which immune regulatory cells dampen cytotoxic immune reactivity while fostering a tolerogenic environment. We further discuss how this understanding of regulatory cells can shape graft engineering and other therapeutic strategies to improve long-term outcomes for patients receiving HSCT and SOT

Plasma activated PDMS microstructured pattern with collagen for improved myoblast cell guidance

We focused on polydimethylsiloxane (PDMS) as a substrate for replication, micropatterning, and construction of biologically active surfaces. The novelty of this study is based on the combina-tion of argon plasma exposure of micropatterned PDMS scaffold, where the plasma served as a strong tool for subsequent grafting of collagen coating and their application as cell growth scaf-folds, where the standard has been significantly exceeded. As part of scaffold design, templates with a patterned microstructure of different dimensions (50 x 50, 50 x 20 and 30 x 30 microns were created by photolithography followed by pattern replication on a PDMS polymer substrate. Subsequently, the prepared microstructured PDMS replicas were coated with a type I collagen layer. The sample preparation was followed by the characterization of material surface properties through various analytical techniques, including scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS). To evaluate the biocompatibility of the produced samples, we conducted studies on the interactions between selected polymer replicas with micro- and nanostructures and mammalian cells. Specif-ically, we utilized mouse myoblasts (C2C12) and our results demonstrate that we achieved excellent cell alignment in conjunction with the development of a cytocompatible surface. Consequently, the outcomes of this research contribute to an enhanced comprehension of surface properties and interactions between structured polymers and mammalian cells. The use of periodic microstructures holds the potential for advancing the creation of novel materials and scaffolds in tissue engineering. These materials exhibit exceptional biocompatibility and possess the capacity to promote cell adhesion and growth

Surface activation of Hastalex by vacuum argon plasma for cytocompatibility enhancement

Here, we present surface analysis and biocompatibility evaluation of novel composite material based on graphene oxide traded as Hastalex. First, the surface morphology and elemental analysis of the pristine material were examined by atomic force and scanning electron microscopies, and by energy-dispersive and X-ray photoelectron spectroscopies, respectively. The Hastalex surface was then modified by plasma, 3 and 8 W with exposure times up to 240 s, the impact of which on the material surface wettability and morphology was further evaluated. In addition, the material aging was studied at room and elevated temperatures. Significant changes in surface roughness, morphology, and area were detected at the nanometre scale after plasma exposure. An increase in oxygen content due to the plasma exposure was observed both for 3 and 8 W. The plasma treatment had an outstanding effect on the cytocompatibility of Hastalex foil treated at both input powers of 3 and 8 W. The cell number of human MRC 5 fibroblasts on Hastalex foils exposed to plasma increased significantly compared to pristine Hastalex and even to tissue culture polystyrene. The plasma exposure also affected the fibroblasts cell growth and shape

LIPSS pattern induced by polymer surface instability for myoblast cell guidance

The presented study highlights the efficiency of employing a KrF excimer laser to create diverse types of periodic nanostructures (LIPSS - laser induced periodic surface structures) on polyether ether ketone (PEEK) and polyethylene naphthalate (PEN) substrates. By exposing the polymer films below their ablation threshold to laser fluence ranging from 4 to 16 mJcm-2 at 6,000 pulses, we studied both single-phase exposure at beam incidence angles of 0deg and 45deg, and two-phase exposure. Atomic force microscopy analysis revealed that the laser-treated samples contained distinctive periodic patterns such as waves, globules, and pod-like structures each exhibiting unique surface roughness. Moreover, using analytical methods like EDS and XPS shed light on the changes in the atomic composition, specifically focusing on the C and O elements, as a result of laser exposure. Notably, in almost all cases, we observed an increase in oxygen percentage on the sample surfaces. This increase not only led to a decrease in the contact angle with water but also lowered the zeta potential value, thus showing that the modified samples have enhanced hydrophilicity of the surface and altered electrostatic properties. Last but not least, the samples were assessed for biocompatibility; we studied the interaction of the prepared replicates with mouse myoblasts (C2C12). The impact of globular/dot structures on the cell growth in comparison to pristine or linear LIPSS-patterned surfaces was determined. The linear pattern (LIPSS) induced the myoblast cell alignment along the pattern direction, while dot/globular pattern even enhanced the cytocompatibility compared to LIPSS samples