TO THE QUESTION OF PSYCHOLOGICAL CULTURE DEVELOPMENT OF DOCTORS IN THE SYSTEM OF ADDITIONAL EDUCATION

The article describes the importance of psychological culture of the doctors professional activity. It presents the results of the pilot research of doctors psychological culture and the experience of its development in the additional education system by the programs of advanced training and professional retraining of doctors. Also a paper provides the basic work strategy with doctors to develop their psychological culture

N\u3csup\u3e1\u3c/sup\u3e-Acetyl-3\u27-methylandrosta-4,16-dieno[16,17-\u3cem\u3ed\u3c/em\u3e]pyrazole-3-one

In an attempt to find the structural features promoting the thermal isomerization of the N-acylated steroid [16,17-d]pyrazoles into [17,16-c]pyrazole derivatives,the X-ray structure analysis of the title compound, C23H30N2O3, (1), has been carried out. The steroid moiety of (I) has the usual conformation. The dihedral angle between the planar pyrazole ring and the N-acetyl group is very small [5.6 (2)°], but the amide C-N bond seems to be substantially elongated [1.404 (3) Å ]. The d-pyrazole ring junction via a double bond leads to deformations of some bond and torsion angles, which would be decreased in the case of a ring junction via a single bond in the [17, 16-c]pyrazole isomer

Subjective Assessment of Father – Teenage Child Interaction

The current dynamic socio-cultural processes lead to a qualitative revision of family roles, family relationships, and parenthood. The psychological interaction between parents has changed, as has the father’s status. This study featured the interaction between 40 fathers and their teenage children. The authors used the PARI method by E. S. Schaefer and R. K. Bell as adapted by T. V. Neshcheret, the Parent-Child Interaction method developed by I. M. Markovskaya, and J. Pleck’s Fatherhood and Masculinity Questionnaire. The consistency was determined by the Spearman’s correlation coefficient while the differences in the assessments provided by fathers and children were measured using the Mann – Whitney U test. For the teenagers, the main interaction indicator appeared to be the lack of strictness whereas their fathers stressed the emotional closeness. The comparative analysis revealed no significant differences between the assessments given by the fathers and the children: the father was described as not strict, accepting, cooperative, and consistent. Emotional closeness proved to be the most significant parameter in the father – teenage child interaction. Emotional closeness requires special attention from specialists that help parents and children overcome the adolescent crisis

Semantic Structure of a Movie Hero Favored by Teenagers

The authors used the psychosemiotic content analysis, projective method, personal differential, and factor analysis to study the semantics of a movie character preferred by teenagers. An incomplete-sentence test and a blog analysis revealed eight categories and nineteen corresponding analysis units based on the occurrence / non-occurrence of selected units in the text and their frequency. Osgood’s matrices revealed the measure of the random coincidence of classification units (≥ 70 %) with other categories. The integrative semantic structure of the favored movie hero was determined by such categories and content units as a) gender (male); b) appearance (look); c) intellect (mental abilities); d) interaction (relations with other people); e) character traits (moral and volitional qualities, attitude to others, attitude to work); f) actions (struggle for justice, readiness to do something for others); g) attitude to the family (willingness to sacrifice with something for the family)

Imaging cytoplasmic cAMP in mouse brainstem neurons

<p>Abstract</p> <p>Background</p> <p>cAMP is an ubiquitous second messenger mediating various neuronal functions, often as a consequence of increased intracellular Ca²⁺levels. While imaging of calcium is commonly used in neuroscience applications, probing for cAMP levels has not yet been performed in living vertebrate neuronal tissue before.</p> <p>Results</p> <p>Using a strictly neuron-restricted promoter we virally transduced neurons in the organotypic brainstem slices which contained pre-Bötzinger complex, constituting the rhythm-generating part of the respiratory network. Fluorescent cAMP sensor Epac1-camps was expressed both in neuronal cell bodies and neurites, allowing us to measure intracellular distribution of cAMP, its absolute levels and time-dependent changes in response to physiological stimuli. We recorded [cAMP]_ichanges in the micromolar range after modulation of adenylate cyclase, inhibition of phosphodiesterase and activation of G-protein-coupled metabotropic receptors. [cAMP]_ilevels increased after membrane depolarisation and release of Ca²⁺from internal stores. The effects developed slowly and reached their maximum after transient [Ca²⁺]_ielevations subsided. Ca²⁺-dependent [cAMP]_itransients were suppressed after blockade of adenylate cyclase with 0.1 mM adenylate cyclase inhibitor 2'5'-dideoxyadenosine and potentiated after inhibiting phosphodiesterase with isobutylmethylxanthine and rolipram. During paired stimulations, the second depolarisation and Ca²⁺release evoked bigger cAMP responses. These effects were abolished after inhibition of protein kinase A with H-89 pointing to the important role of phosphorylation of calcium channels in the potentiation of [cAMP]_itransients.</p> <p>Conclusion</p> <p>We constructed and characterized a neuron-specific cAMP probe based on Epac1-camps. Using viral gene transfer we showed its efficient expression in organotypic brainstem preparations. Strong fluorescence, resistance to photobleaching and possibility of direct estimation of [cAMP] levels using dual wavelength measurements make the probe useful in studies of neurons and the mechanisms of their plasticity. Epac1-camps was applied to examine the crosstalk between Ca²⁺and cAMP signalling and revealed a synergism of actions of these two second messengers.</p