Freshwater jellyfish Craspedacusta sowerbyi Lankester, 1880 (Hydrozoa, Olindiidae): 50 years' observations in Serbia

Detailed and relevant limnological investigations of Serbian waters were initiated in 1958 and have continued to the present. During the period 1971-2008 we monitored biological elements as a part of working studies/projects, including the distribution of the freshwater jellyfish Craspedacusta sowerbyi Lankester, 1880. We observed over 500 sampling sites in running and standing waters. Specimens of this hydro-medusa were found in five of them. Throughout the period of investigation, only the medusae stages were observed. Our purpose in this paper was to provide data of the records and distribution of this limnomedusa during the period 1958-2008 in inland waters of Serbia. These observations should contribute to knowledge on the limnofauna not only of the Balkan Peninsula but Europe as a whole.Opsežna i značajna limnološka istraživanja voda u Srbiji otpočela su 1958. godine i nastavljena do danas. Tokom celog istraživačkog perioda nađena je slatkovodna meduza Craspedacusta sowerbyi Lankester, 1880 na pet lokaliteta (bara na Velikoj Moravi kod Ćuprije, Savsko jezero kod Beograda, jezero Velika Peščara kod Zrenjanina, jezero Miloševo kod Jagodine i jezero Šumarice kod Kragujevca). Predstavljeni rezultati su doprinos dosadašnjih saznanja o nalazima i distribuciji ove vrste u Srbiji, kao i poznavanju limnofaune Balkanskog poluostrva i Evrope.Projekat ministarstva br. 14302

Nest-site preference of griffon vulture (gyps fulvus) in Herzegovina

Although formerly an abundant species, the Eurasian Griffon (Gyps fulvus Hablizl, 1783) has undergone a dramatic decline in Herzegovina. Such an unfavorable trend may be associated with frequent poisoning incidents (consumption of poisoned baits), shortage of food and hunting. This species disappeared from its breeding habitats in Herzegovina during the last decade of the 20th century. The extinction was probably caused by military activities during the civil war. Using data that were collected over a period of long-term (1980-1991) monitoring of the breeding population, we discovered optimal environmental conditions for the nesting of the Eurasian Griffon Vulture in Herzegovina. Information on nest-site preference is valuable for conservation programs and the possible reintroduction of the Eurasian Griffon, not only in Herzegovina, but also to a much wider region. During the study period, we observed 61 nests and 252 nesting cases in four colonies of Eurasian Griffon Vulture. Most nests were located on limestone and dolomite rocks. The average altitude of nests was 378 m a.s.l.; most of nests (85%) were located below 500 m a.s.l. Also, the majority of nests were located on west-exposed sites

Freshwater jellyfish craspedacusta sowerbyi lankester, 1880 (hydrozoa, olindiidae)-50 years' observations in Serbia

Detailed and relevant limnological investigations of Serbian waters were initiated in 1958 and have continued to the present. During the period 1971-2008 we monitored biological elements as a part of working studies/projects, including the distribution of the freshwater jellyfish Craspedacusta sowerbyi Lankester, 1880. We observed over 500 sampling sites in running and standing waters. Specimens of this hydro-medusa were found in five of them. Throughout the period of investigation, only the medusae stages were observed. Our purpose in this paper was to provide data of the records and distribution of this limnomedusa during the period 1958-2008 in inland waters of Serbia. These observations should contribute to knowledge on the limnofauna not only of the Balkan Peninsula but Europe as a whole

Chemosensory Responses to the Repellent Nepeta Essential Oil and Its Major Component Nepetalactone by Aedes aegypti (Diptera: Culicidae), a Vector of Zika Virus

Nepeta essential oil (Neo; catnip) and its major component, nepetalactone, have long been known to repel insects including mosquitoes. However, the neural mechanisms through which these repellents are detected by mosquitoes, including the yellow fever mosquito Aedes aegypti (L.), an important vector of Zika virus, were poorly understood. Here we show that Neo volatiles activate olfactory receptor neurons within the basiconic sensilla on the maxillary palps of female Ae. aegypti. A gustatory receptor neuron sensitive to the feeding deterrent quinine and housed within sensilla on the labella of females was activated by both Neo and nepetalactone. Activity of a second gustatory receptor neuron sensitive to the feeding stimulant sucrose was suppressed by both repellents. Our results provide neural pathways for the reported spatial repellency and feeding deterrence of these repell ents. A better understanding of the neural input through which female mosquitoes make decisions to feed will facilitate design of new repellents and management strategies involving their use.The final publication is now available at: [https://doi.org/10.1093/jme/tjx059

Different aspect of sustainable use of fish resources in Serbia for the period 2006-2017

Ovaj rad ima za cilj da ustanovi koja je uloga zakona u dobrom upravljanju ribolovnim resursima. Zaštita i održivo korišćenje ribljeg fonda u Srbiji regulisano je istoimenim zakonom, a odvija se na 17 ribarskih područja i 29 ribarskih područja u zaštićenim prirodnim dobrima. Na 17 osnovnih ribarskih područja upravljaju „javna preduzeća“ ili privatne firme u statusu „doo“. Kod većih područja, posebno u Vojvodini, postoji više upravljača za jedno ribarsko područje. U zaštićenim prirodnim dobrima upravljači su ujedno i korisnici ribljeg fonda. U periodu od 2006. do 2017. godine Zakon o zaštiti i održivom korišćenju ribljeg fonda menjan je u dva navrata: 2009. i 2014. godine. Podzakonska akta su takođe podložna vrlo čestim promenama. U radu se prate efekti promene zakonske regulative na broj rekre ativnih i profesionalnih ribolovaca, broj ribočuvara i trendove u ulovima. Osnovni trendovi su da broj rekreativnih ribolovaca u navedenom periodu opada, broj profesionalnih riba ra stagnira, dok broj ribočuvara raste. Ulov ima tendenciju opadanja za sve vrste koje se statististički prate (šaran, som, smuđ i deverika od autohtonih vrsta, i babuška i tolstolobik od alohtonih). Odnos ulova u rekreativnom i komercijalnom ribolovu pokazuje značajne promene u poslednjih desetak godina: osim za tolstolobika, kod koga dominira komer cijalni ulov, kod ostalih vrsta je došlo do zamene u smislu da rekreativni ulov preuzima dominaciju u poslednjih nekoliko godin

Clinical autonomic nervous system laboratories in Europe: a joint survey of the European Academy of Neurology and the European Federation of Autonomic Societies

© 2022 The Authors. European Journal of Neurology published by John Wiley & Sons Ltd on behalf of European Academy of Neurology. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.Background and purpose: Disorders of the autonomic nervous system (ANS) are common conditions, but it is unclear whether access to ANS healthcare provision is homogeneous across European countries. The aim of this study was to identify neurology-driven or interdisciplinary clinical ANS laboratories in Europe, describe their characteristics and explore regional differences. Methods: We contacted the European national ANS and neurological societies, as well as members of our professional network, to identify clinical ANS laboratories in each country and invite them to answer a web-based survey. Results: We identified 84 laboratories in 22 countries and 46 (55%) answered the survey. All laboratories perform cardiovascular autonomic function tests, and 83% also perform sweat tests. Testing for catecholamines and autoantibodies are performed in 63% and 56% of laboratories, and epidermal nerve fiber density analysis in 63%. Each laboratory is staffed by a median of two consultants, one resident, one technician and one nurse. The median (interquartile range [IQR]) number of head-up tilt tests/laboratory/year is 105 (49-251). Reflex syncope and neurogenic orthostatic hypotension are the most frequently diagnosed cardiovascular ANS disorders. Thirty-five centers (76%) have an ANS outpatient clinic, with a median (IQR) of 200 (100-360) outpatient visits/year; 42 centers (91%) also offer inpatient care (median 20 [IQR 4-110] inpatient stays/year). Forty-one laboratories (89%) are involved in research activities. We observed a significant difference in the geographical distribution of ANS services among European regions: 11 out of 12 countries from North/West Europe have at least one ANS laboratory versus 11 out of 21 from South/East/Greater Europe (p = 0.021). Conclusions: This survey highlights disparities in the availability of healthcare services for people with ANS disorders across European countries, stressing the need for improved access to specialized care in South, East and Greater Europe.info:eu-repo/semantics/publishedVersio

EFAS/EAN survey on the influence of the COVID-19 pandemic on European clinical autonomic education and research

© The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.Purpose: To understand the influence of the coronavirus disease 2019 (COVID-19) pandemic on clinical autonomic education and research in Europe. Methods: We invited 84 European autonomic centers to complete an online survey, recorded the pre-pandemic-to-pandemic percentage of junior participants in the annual congresses of the European Federation of Autonomic Societies (EFAS) and European Academy of Neurology (EAN) and the pre-pandemic-to-pandemic number of PubMed publications on neurological disorders. Results: Forty-six centers answered the survey (55%). Twenty-nine centers were involved in clinical autonomic education and experienced pandemic-related didactic interruptions for 9 (5; 9) months. Ninety percent (n = 26/29) of autonomic educational centers reported a negative impact of the COVID-19 pandemic on education quality, and 93% (n = 27/29) established e-learning models. Both the 2020 joint EAN-EFAS virtual congress and the 2021 (virtual) and 2022 (hybrid) EFAS and EAN congresses marked higher percentages of junior participants than in 2019. Forty-one respondents (89%) were autonomic researchers, and 29 of them reported pandemic-related trial interruptions for 5 (2; 9) months. Since the pandemic begin, almost half of the respondents had less time for scientific writing. Likewise, the number of PubMed publications on autonomic topics showed the smallest increase compared with other neurological fields in 2020-2021 and the highest drop in 2022. Autonomic research centers that amended their trial protocols for telemedicine (38%, n = 16/41) maintained higher clinical caseloads during the first pandemic year. Conclusions: The COVID-19 pandemic had a substantial negative impact on European clinical autonomic education and research. At the same time, it promoted digitalization, favoring more equitable access to autonomic education and improved trial design.info:eu-repo/semantics/publishedVersio

EFAS/EAN survey on the influence of the COVID-19 pandemic on European clinical autonomic education and research

Purpose: To understand the influence of the coronavirus disease 2019 (COVID-19) pandemic on clinical autonomic education and research in Europe. Methods: We invited 84 European autonomic centers to complete an online survey, recorded the pre-pandemic-to-pandemic percentage of junior participants in the annual congresses of the European Federation of Autonomic Societies (EFAS) and European Academy of Neurology (EAN) and the pre-pandemic-to-pandemic number of PubMed publications on neurological disorders. Results: Forty-six centers answered the survey (55%). Twenty-nine centers were involved in clinical autonomic education and experienced pandemic-related didactic interruptions for 9 (5; 9) months. Ninety percent (n = 26/29) of autonomic educational centers reported a negative impact of the COVID-19 pandemic on education quality, and 93% (n = 27/29) established e-learning models. Both the 2020 joint EAN-EFAS virtual congress and the 2021 (virtual) and 2022 (hybrid) EFAS and EAN congresses marked higher percentages of junior participants than in 2019. Forty-one respondents (89%) were autonomic researchers, and 29 of them reported pandemic-related trial interruptions for 5 (2; 9) months. Since the pandemic begin, almost half of the respondents had less time for scientific writing. Likewise, the number of PubMed publications on autonomic topics showed the smallest increase compared with other neurological fields in 2020-2021 and the highest drop in 2022. Autonomic research centers that amended their trial protocols for telemedicine (38%, n = 16/41) maintained higher clinical caseloads during the first pandemic year. Conclusions: The COVID-19 pandemic had a substantial negative impact on European clinical autonomic education and research. At the same time, it promoted digitalization, favoring more equitable access to autonomic education and improved trial design

Lectura y autocontrol en la ortopedia de las pasiones

Random amplified polymorphic DNA (RAPD) analysis is a simple and reliable method used to detect DNA polymorphism. Several factors can affect the amplification profiles, thereby causing false bands and non-reproducibility of the assay. In this study, we analyzed the effects of different concentrations of primer, magnesium chloride, template DNA and Taq DNA polymerase to develop and standardize a RAPD protocol for Centaurium species. The optimized PCR reaction mixture included: 50 ng of DNA extracted using a CTAB based protocol, 2.5 mM MgCl2, 7.5 pmol primer and 2 U of Taq polymerase in a final volume of 25 μl. Each of the five primers used in experiments (OPB11, OPB15, OPB18, OPF05 and OPH02) generated reproducible and distinguishable fingerprinting patterns of four Centaurium species. The obtained optimized RAPD protocol and the selected primers are useful for our further work in the genetic diversity studies of Centaurium species.Projekat ministarstva br. 17302