Single-cell Motility Analysis of Tethered Human Spermatozoa.

Vigorous sperm flagellar motility is essential for fertilization, and so the quantitative measurement of motility is a useful tool to assess the intrinsic fertility potential of sperm cells and explore how various factors can alter sperm's ability to reach the egg and penetrate its protective layers. Human sperm beat their flagella many times each second, and so recording and accurately quantifying this movement requires a high-speed camera. The aim of this protocol is to provide a detailed description of the tools required for quantitative beat frequency measurement of tethered human sperm at the single-cell level and to describe methods for investigating the effects of intracellular or extracellular factors on flagellar motion. This assay complements bulk measurements of sperm parameters using commercially-available systems for computer-assisted sperm analysis (CASA)

The New Zealand Strong Motion Earthquake Recorder Network

The network of strong-motion earthquake recorders, maintained throughout New Zealand by the Engineering Seismology Section of the Department of Scientific and Industrial Research, is described. The instruments are either deployed as ground instruments to measure potential earthquake attack on structures, or in structures, e.g. buildings, dams and industrial installations, to record structural response. Details are given of installation of instruments , maintenance, laboratory work, record retrieval and digitisation, costs and staffing for the network. Future developments mooted include an improved digitising system, the introduction of an improved version of the existing mechanical-optical instrument in 1979, and, in the long term, the introduction of an entirely new digital recorder, having an electrical output from its accelerometers, which will make possible the transmission of data by telephone or radio link

A model of large volumetric capacitance in graphene supercapacitors based on ion clustering

Electric double layer supercapacitors are promising devices for high-power energy storage based on the reversible absorption of ions into porous, conducting electrodes. Graphene is a particularly good candidate for the electrode material in supercapacitors due to its high conductivity and large surface area. In this paper we consider supercapacitor electrodes made from a stack of graphene sheets with randomly-inserted "spacer" molecules. We show that the large volumetric capacitances C > 100 F/cm^3 observed experimentally can be understood as a result of collective intercalation of ions into the graphene stack and the accompanying nonlinear screening by graphene electrons that renormalizes the charge of the ion clusters.Comment: 13 pages, 5 figures; additional discussion and supporting calculations adde

X-rays from T Tau: A test case for accreting T Tauri stars

We test models for the generation of X-rays in accreting T Tauri stars (TTS), using X-ray data from the classical TTS T Tau. High-resolution spectroscopy from the Reflection Grating Spectrometers on XMM-Newton is used to infer electron densities, element abundances and the thermal structure of the X-ray source. We also discuss the ultraviolet light curve obtained by the Optical Monitor, and complementary ground-based photometry. A high-resolution image from Chandra constrains contributions from the two companions of T Tau N. The X-ray grating spectrum is rich in emission lines, but shows an unusual mixture of features from very hot (~30 MK) and very cool (1-3 MK) plasma, both emitted by similar amounts of emission measure. The cool plasma confirms the picture of a soft excess in the form of an enhanced OVII/OVIII Lya flux ratio, similar to that previously reported for other accreting TTS. Diagnostics from lines formed by this plasma indicate low electron densities (<~ 1E10 cm-3). The Ne/Fe abundance ratio is consistent with a trend in pre-main sequence stars in which this ratio depends on spectral type, but not on accretion. On the basis of line density diagnostics, we conclude that the density of the cool ``soft-excess'' plasma is orders of magnitude below that predicted for an accretion shock, assuming previously determined accretion rates of (3-6)E-8 M_sun/y. We argue that loading of magnetic field lines with infalling material suppresses the heating process in a part of the corona. We thus suggest that the X-ray production of T Tau is influenced by the accretion process although the X-rays may not form in the bulk of the accretion footpoints.Comment: 12 pages, 7 figures, A&A style. Accepted by A&A, to appear in a special section/issue dedicated to the XMM-Newton Extended Survey of the Taurus Molecular Cloud (XEST). See also http://www.issibern.ch/teams/Taurus/papers.htm

Investigation of Graded La2NiO4+ Cathodes to Improve SOFC Electrochemical Performance

Mixed ionic and electronic conducting MIEC oxides are promising materials for use as cathodes in solid oxide fuel cells SOFCs due to their enhanced electrocatalytic activity compared with electronic conducting oxides. In this paper, the MIEC oxide La2NiO4+ was prepared by the sol-gel route. Graded cathodes were deposited onto yttria-stabilized zirconia YSZ pellets by dip-coating, and electrochemical impedance spectroscopy studies were performed to characterize the symmetrical cell performance. By adapting the slurries, cathode layers with different porosities and thicknesses were obtained. A ceria gadolinium oxide CGO barrier layer was introduced, avoiding insulating La2Zr2O7 phase formation and thus reducing resistance polarization of the cathode. A systematic correlation between microstructure, composition, and electrochemical performance of these cathodes has been performed. An improvement of the electrochemical performance has been demonstrated, and a reduction in the area specific resistance ASR by a factor of 4.5 has been achieved with a compact interlayer of La2NiO4+ between the dense electrolyte and the porous La2NiO4+ cathode layer. The lowest observed ASR of 0.11 cm2 at 800°C was obtained from a symmetrical cell composed of a YSZ electrolyte, a CGO interlayer, an intermediate compact La2NiO4+ layer, a porous La2NiO4+ electrode layer, and a current collection layer of platinum paste

Shock tunnel studies of scramjet phenomena, supplement 6

Reports by the staff of the University of Queensland on various research studies related to the advancement of scramjet technology are presented. These reports document the tests conducted in the reflected shock tunnel T4 and supporting research facilities that have been used to study the injection, mixing, and combustion of hydrogen fuel in generic scramjets at flow conditions typical of hypersonic flight. In addition, topics include the development of instrumentation and measurement technology, such as combustor wall shear and stream composition in pulse facilities, and numerical studies and analyses of the scramjet combustor process and the test facility operation

Provenance and geochemistry of exotic clasts in conglomerates of the Oligocene Torehina Formation, Coromandel Peninsula, New Zealand

Non-marine pebble to cobble conglomerates of the lower Torehina Formation (Oligocene) crop out along western Coromandel Peninsula and overlie, with strong angular discordance, continental-margin metasedimentary rocks (Manaia Hill Group) of Mesozoic (Late Jurassic to ?Early Cretaceous) age. The conglomerates contain provenance information that identifies a pre-Oligocene depositional history obscured by the unconformable juxtaposition of these Tertiary and Mesozoic strata. Most clasts in the lower Torehina Formation are visually similar to local bedrock lithologies, including metamorphosed sandstones and argillites, but are kaolinitic and contain more detrital and authigenic chert, quartz, and potash feldspar. Local derivation of these clasts seems unlikely. By comparing geochemical ratios with those defined for continental margin sandstones, and well characterised New Zealand tectonic terranes, we interpret the majority of clasts in the lower Torehina Formation to have been derived from a dissected orogen, with mixtures of felsic and volcanogenic-derived sediment. The most likely sources are the Waipapa and Torlesse Terranes. The remaining 20–30% of the clasts in the lower Torehina Formation were originally friable, are coarse grained, and appear to be lithologically exotic relative to known metamorphosed sandstones in basement terrane sources on North Island. Some clasts contain coal laminae and particles, and all contain detrital kaolinite as lithic fragments and matrix. Such characteristics imply a non-marine to marginal-marine source containing sediment derived from strongly weathered granite or granodiorite. Mechanical fragility implies a likely proximal, easily erodible source. We propose that this group of clasts was derived from an Upper Cretaceous sedimentary cover, either part of a locally developed basin fill or part of a once regionally extensive cover on North Island. Either case defines a more widely distributed Cretaceous source than found today