Ovine pedomics : the first study of the ovine foot 16S rRNA-based microbiome

We report the first study of the bacterial microbiome of ovine interdigital skin based on 16S rRNA by pyrosequencing and conventional cloning with Sanger-sequencing. Three flocks were selected, one a flock with no signs of footrot or interdigital dermatitis, a second flock with interdigital dermatitis alone and a third flock with both interdigital dermatitis and footrot. The sheep were classified as having either healthy interdigital skin (H), interdigital dermatitis (ID) or virulent footrot (VFR). The ovine interdigital skin bacterial community varied significantly by flock and clinical condition. The diversity and richness of operational taxonomic units was greater in tissue from sheep with ID than H or VFR affected sheep. Actinobacteria, Bacteriodetes, Firmicutes and Proteobacteria were the most abundant phyla comprising 25 genera. Peptostreptococcus, Corynebacterium and Staphylococcus were associated with H, ID and VFR respectively. Sequences of Dichelobacter nodosus, the causal agent of ovine footrot, were not amplified due to mismatches in the 16S rRNA universal forward primer (27F). A specific real time PCR assay was used to demonstrate the presence of D. nodosus which was detected in all samples including the flock with no signs of ID or VFR. Sheep with ID had significantly higher numbers of D. nodosus (104-109 cells/g tissue) than those with H or VFR feet

Management of consecutive cuts in the production and quality of wintergreen paspalum seeds

The objective of this study was to evaluate the practice of consecutive cuts in the production and quality of Paspalum guenoarum seeds of the “Azulão” ecotype. The experimental design used was in completely randomized blocks with four replications. The treatment included three cuts in succession: zero cut, one cut, two cuts, and three cuts. The variables were: number of total tillers/plant; number of vegetative tillers/plant; number of reproductive tillers/plant; percentage of reproductive tillers; number of racemes/inflorescence; weight of thousand seeds; number of seeds/inflorescence; seed production; forage dry matter; water content of seeds; germination; first germination count; and germination speed index. The largest seed production was obtained with zero cut (850.3, first year and 719.4 kg/ha–1, second year) and one cut (794.4, first year and 627.3 kg/ha–1, second year) with no statistical difference between them. The largest germination percentage was seen with the application of zero (71.0%, first year and 79.3%, second year) and one cut (69.3%, first year and 75.0%, second year). There was a decrease in the production and quality of the seeds of the second cut, especially during the first year of evaluation. The total tillers, the percentage of tillers that went through the reproductive stage, and the number of reproductive tillers are the variables that are most highly correlated with seed production